Serum antibody response against periodontal bacteria and coronary heart disease: Systematic review and meta-analysis.

AIM: The present systematic review and meta-analysis assessed the strength of a reported association between elevated serum anti-periodontal bacterial antibody responses and coronary heart disease (CHD). MATERIALS AND METHODS: Twenty original studies were identified after systematically searching five databases. The majority (n = 11) compared serum anti-Porphyromonas gingivalis (Pg) and/or anti-Aggregatibacter actinomycetemcomitans (Aa) IgG antibody responses between CHD patients and control participants. The strength of the association between serum anti-Pg antibodies and CHD (n = 10) and serum anti-Aa antibodies and CHD (n = 6) was investigated using a meta-analysis approach separately. RESULTS: Most studies (61%) reported that the serum IgG antibody responses were elevated in CHD patients than in controls. The meta-analyses showed a significant association between elevated serum IgG antibody responses (anti-Pg and anti-Aa) and CHD, with pooled odds ratios of 1.23 [95% confidence interval (CI): 1.09-1.38, p = .001] and 1.25 (95% CI: 1.04-1.47, p = .0004), respectively. CONCLUSIONS: A modest increase of CHD events in individuals with higher serum anti-Pg and anti-Aa IgG antibody responses may support their use as potential biomarkers to detect and monitor at-risk populations. However, the observed inconsistencies with the design and interpretation of immunoassays warrant standardization of the immunoassays assessing antibody responses against periodontal bacteria.

Detection of periodontal microorganisms in coronary atheromatous plaque specimens of myocardial infarction patients: A systematic review and meta-analysis.

BACKGROUND: Microbial translocation from inflamed periodontal pockets into coronary atheroma via systemic circulation is one of the proposed pathways that links periodontitis and myocardial infarction (MI). The purpose of this systematic review is to determine the reported prevalence of periodontal microorganisms in coronary atheroma and/or aspirated clot samples collected from MI patients with periodontal disease. METHODOLOGY: The "Preferred Reporting Items for Systematic Reviews and Meta-Analyses" (PRISMA) guidelines were followed. Six databases were systematically searched using Medical Subject Headings/Index and Entree terms. After a thorough screening, fourteen publications spanning over ten years (2007-2017) were eligible for this systematic review and meta-analysis. RESULTS: Out of 14 included studies, 12 reported presence of periodontal bacterial DNA in coronary atherosclerotic plaque specimens. Overall, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans were the most frequently detected periodontal bacterial species. Meta-analysis revealed that the prevalence of P. gingivalis was significantly higher than A. actinomycetemcomitans in coronary atheromatous plaque samples. Apart from periodontal microbes, DNA from a variety of other microbes e.g. Pseudomonas fluorescens, Streptococcus species, Chlamydia pneumoniae were also recovered from the collected samples. CONCLUSION: Consistent detection of periodontal bacterial DNA in coronary atheroma suggests their systemic dissemination from periodontal sites. It should further be investigated whether they are merely bystanders or induce any structural changes within coronary arterial walls.

The oral health status of patients with peripheral vascular disorders: A systematic review.

OBJECTIVES: Periodontal disease and tooth loss were found to be associated with several peripheral vascular disorders. Nonetheless, an evaluation of the literature on the broader domains of oral health in individuals with peripheral vascular disorders is lacking. This systematic review aims to collate the current evidence on the oral health status of individuals with peripheral vascular disorders. METHODS: Five electronic databases were searched for studies assessing oral health parameters in individuals with peripheral vascular disorders. Outcome measures considered were periodontal health, dentition status, caries indices, oral prostheses, oral pathologies and oral hygiene behaviours. The Newcastle-Ottawa scale was used to appraise the quality of the studies. RESULTS: From 3025 records identified, 24 studies involving 1232 participants with peripheral vascular disorders were included in this review. In nine studies, periodontitis was significantly more prevalent in peripheral vascular disorders compared to non-peripheral vascular disorders participants. A further six studies reported individuals with peripheral vascular disorders also had significantly fewer teeth and increased rates of edentulism. Only one study reported a higher incidence of dental caries in peripheral vascular disorders participants. Other aspects of oral health such as oral prosthesis, oral pathology and oral hygiene behaviours were seldom assessed. CONCLUSIONS: The scarcity of studies reporting on broader domains limited our ability to arrive at a conclusion regarding the oral health status of individuals with peripheral vascular disorders. Future studies ought to assess these domains in individuals with peripheral vascular disorders and controls to gain a more complete understanding of oral health and its potential association with peripheral vascular disorders.

The link between ankylosing spondylitis and oral health conditions: two nested case-control studies using data of the UK Biobank

Abstract Ankylosing Spondylitis (AS) is an inflammatory rheumatic disease that affects the axial skeleton and the sacroiliac joints. Recent studies investigated the link between AS and oral diseases, particularly periodontitis. Others suggested that periodontitis may have a role in the pathogenesis of rheumatic diseases. Objective: The aim of this study is to investigate the association between AS and oral conditions. Material and Methods: This research was conducted using the UK Biobank Resource under Application Number 26307. The UK Biobank recruited around 500000 participants throughout Great Britain. Clinical records were available for 2734 participants. Two case-control studies were conducted based on whether AS was self-reported or clinically diagnosed. Oral conditions were identified using self-reported reports of oral ulcers, painful gums, bleeding gums, loose teeth, toothache, and dentures. The association between AS and oral conditions was assessed using logistic regression adjusted for age, gender, educational level, smoking status, alcohol consumption, and body mass index. Results: A total of 1307 cases and 491503 control participants were eligible for the self-reported AS study. The mean age was 58 years for the cases [7.5 standard deviation (SD)] and 57 years for the control groups (8.1 SD). Also, 37.1% of the cases and 54.2% of the control participants were females. Among the oral conditions, only oral ulcers were strongly associated with AS [1.57 adjusted odds ratio (OR); 95% confidence interval (CI) 1.31 to 1.88]. For the study of clinically diagnosed AS, 153 cases and 490351 control participants were identified. The mean age for both cases and control groups was 57 years; 7.6 SD for the cases and 8.1 for the control group. Females corresponded to 26.1% of the cases, and 54.2% of the control participants. Clinically diagnosed AS was associated with self-reported oral ulcers (2.17 adjusted OR; 95% CI 1.33 to 3.53). Conclusion: Self-reported and clinically diagnosed AS populations have increased risk of reporting oral ulcers. Further investigations are required to assess the link between a specific type of oral condition and AS.

The link between ankylosing spondylitis and oral health conditions: two nested case-control studies using data of the UK Biobank.

Ankylosing Spondylitis (AS) is an inflammatory rheumatic disease that affects the axial skeleton and the sacroiliac joints. Recent studies investigated the link between AS and oral diseases, particularly periodontitis. Others suggested that periodontitis may have a role in the pathogenesis of rheumatic diseases. OBJECTIVE: The aim of this study is to investigate the association between AS and oral conditions. MATERIAL AND METHODS: This research was conducted using the UK Biobank Resource under Application Number 26307. The UK Biobank recruited around 500000 participants throughout Great Britain. Clinical records were available for 2734 participants. Two case-control studies were conducted based on whether AS was self-reported or clinically diagnosed. Oral conditions were identified using self-reported reports of oral ulcers, painful gums, bleeding gums, loose teeth, toothache, and dentures. The association between AS and oral conditions was assessed using logistic regression adjusted for age, gender, educational level, smoking status, alcohol consumption, and body mass index. RESULTS: A total of 1307 cases and 491503 control participants were eligible for the self-reported AS study. The mean age was 58 years for the cases [7.5 standard deviation (SD)] and 57 years for the control groups (8.1 SD). Also, 37.1% of the cases and 54.2% of the control participants were females. Among the oral conditions, only oral ulcers were strongly associated with AS [1.57 adjusted odds ratio (OR); 95% confidence interval (CI) 1.31 to 1.88]. For the study of clinically diagnosed AS, 153 cases and 490351 control participants were identified. The mean age for both cases and control groups was 57 years; 7.6 SD for the cases and 8.1 for the control group. Females corresponded to 26.1% of the cases, and 54.2% of the control participants. Clinically diagnosed AS was associated with self-reported oral ulcers (2.17 adjusted OR; 95% CI 1.33 to 3.53). CONCLUSION: Self-reported and clinically diagnosed AS populations have increased risk of reporting oral ulcers. Further investigations are required to assess the link between a specific type of oral condition and AS.

Oral Health and Risk of Arthritis in the Scottish Population: Results from the Scottish Health Survey.

OBJECTIVES: To investigate the link between self-reported oral health and arthritis in the Scottish population using data from the Scottish Health Survey. MATERIAL AND METHODS: Data were available from 2008 to 2013 on self-reported arthritis, oral health conditions and oral hygiene habits from the Scottish Health Survey. Arthritis was defined in this survey by self-reported long standing illness, those who reported having arthritis, rheumatism and/or fibrositis. Oral conditions were defined by self-reported bleeding gums, toothache, biting difficulties and/or edentulousness. Oral hygiene habits were defined by self-reported brushing teeth and/or using dental floss on daily basis. Logistic regression was used for statistical analysis adjusted for age, gender, qualification, smoking and body mass index. RESULTS: Prevalence of self-reported arthritis was 9.3% (95% confidence interval [CI] = 9.03 to 9.57). Those who reported having bleeding gums (adjusted odds ratio [OR] = 1.63; 95% CI = 1.35 to 1.96), toothache (OR = 1.32; 95% CI = 1.16 to 1.5), biting difficulties (OR = 1.95; 95% CI = 1.62 to 2.34), and being edentulous (OR = 1.22; 95% CI = 1.08 to 1.37) had an increased risk of arthritis. Brushing teeth (OR = 1.25; 95% CI = 0.74 to 2.12), and using dental floss (OR = 1.11; 95% CI = 0.89 to 1.39) were not associated with arthritis. CONCLUSIONS: Self-reported oral conditions were associated with increased risk of self-reported arthritis. Oral hygiene habits were not associated with self-reported arthritis. Further investigation is required to assess the causal association between oral hygiene, oral disease and arthritis.

Validity of Self-Reported Periodontal Disease: A Systematic Review and Meta-Analysis.

BACKGROUND: Periodontal disease (PdD) has been shown to be related to other systemic diseases. However, to assess this relationship, large epidemiologic studies are required. Such studies need validated self-report measures. The aim of this systematic review is to assess the validity of self-reported measures in the diagnosis of PdD. METHODS: The review followed Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Medline, Embase, and Google Scholar were searched up to January 2016. Two periodontal journals were searched manually. Two reviewers independently made selected studies and extracted data. All disagreements were resolved after discussion with a third reviewer. Risk of bias was evaluated. Sensitivity, specificity, diagnostic odds ratio, and 95% confidence interval (CI) were calculated. Of 933 papers found, 11 were selected for the review. All studies, except two, had acceptable quality. Four comparable studies were selected for meta-analysis. RESULTS: Study size ranged from 114 to 1,426 participants. Sensitivity and specificity ranged from 4% to 93% and 58% to 94%, respectively. Diagnostic odds ratio was 1.4 (95% CI: 0.9 to 2.2) for the question on bleeding gums and 11.7 (95% CI: 4.1 to 33.4) for the question on tooth mobility. Heterogeneity was low for most questions except those on painful gums and tooth mobility. CONCLUSIONS: Self-reported PdD has acceptable validity and can be used for surveillance of PdD in large epidemiologic studies. However, there is a need for large, well-designed diagnostic studies.

Quantification of residual dentine thickness following crown preparation.

OBJECTIVES: Pulpal response to tooth preparation is a major concern in fixed prosthodontics. Research has suggested that 2mm or more of remaining dentine is critical in protecting the pulp following tooth preparation. However, clinicians have no means of knowing dentine thickness either before or after preparation and therefore lack feedback about this important aspect of preparation quality. The aim of this project was to develop a method for measuring local dentine thickness following tooth preparation for metal ceramic crowns, in vitro, which could be used as a tool to evaluate preparation technique and instrumentation. METHODS: Microtomography (XMT or micro-CT) scans were taken of extracted teeth before and after crown preparation. Local dentine thickness was defined for every voxel within the 3D tooth image as the sum of distances from that voxel to the pulp and to the anatomical surface. The method also allows the thickness of material removed to be quantified. Three-dimensional colour-coded maps of dentine thickness were generated, and the distributions of dentine thickness throughout the teeth were analysed. This was tested by a single operator on sixteen extracted upper central incisors. RESULTS: This method enabled clear visualisation and analysis of residual dentine thickness. In the trial, it revealed consistent over-prepared regions along the labial proximal line angles which, in a clinical case, could affect subsequent tooth and restoration longevity. All but one of the prepared teeth had regions with a residual dentine thickness of less than 1.5mm, in 6 it was less than 1.0mm and in 3 of these it was less than 0.5mm. CONCLUSION: Although ex vivo, this method can be used as a research tool to look for patterns of over- or under-preparation, leading to possible modification of technique, instrumentation and, or crown design. CLINICAL SIGNIFICANCE: It is not currently possible for clinicians to know the thickness of residual dentine following crown preparation, a key factor in long term outcome. The described method of quantifying and visualising this thickness allows preparation techniques and instrumentation to be evaluated in vitro, leading to prospective improvements in clinical procedures.

Consistency of labial finish line preparation for metal ceramic crowns: an investigation of a new bur.

PURPOSE: Previous studies have reported on the difficulties inherent in preparing the labial aspect of teeth for metal ceramic crowns with consistency and also the implications for the definitive restoration of underprepared and overangled finish lines. In this study, a bur designed to prepare a 1.2-mm deep chamfer was tested and compared with two other bur kits. MATERIALS AND METHODS: Seventy-two teeth were prepared to receive metal ceramic crowns in vitro by students using one of the two bur kits or the new bur. Using a coordinate measuring machine (CMM), replicas of the prepared teeth were scanned in the mid-labial plane, and shoulder width and cavosurface angles were measured. RESULTS: The new bur produced preparations with a mean shoulder width and cavosurface angle of 1.146 +/- 0.241 mm and 108 +/- 11 degrees, respectively, compared with 0.626 +/- 0.234 mm and 128 +/- 14 degrees produced by a commercially available standardized crown cutting bur system and 0.626 +/- 0.218 mm and 124 +/- 15 degrees produced by the bur kit in use at our center at the time of the study. These differences were statistically significant at the 5% level, using Tukey's comparison of means. CONCLUSIONS: Teeth prepared by the new bur had wider shoulders and lower cavosurface angles than teeth prepared either with a standardized crown cutting system or locally selected bur kit. The quality of labial preparations produced by the new bur in terms of closeness to the postulated "ideal" width and angulation warrants further investigation.

Dentin exposure in tooth preparations for porcelain veneers: a pilot study.

STATEMENT OF PROBLEM: The various clinical techniques available for even reduction of a tooth surface to receive a porcelain veneer restoration do not produce a consistently even conservative reduction. In addition, it is not known which technique is most conservative of enamel. PURPOSE: The purpose of this pilot study was to assess the effectiveness of 3 clinical techniques, namely, dimple, depth groove, and freehand, in producing an intraenamel preparation. The relation between overpreparation beyond the commonly accepted depth of preparation of 0.5 mm and dentin exposure was also examined. MATERIAL AND METHODS: A single operator prepared 3 groups of 5 extracted maxillary central incisors to a depth of 0.5 mm using dimple, depth-groove, and freehand methods of tooth preparation. The prepared teeth were scanned using an x-ray microtomography scanner. The reconstructed images were studied using software that provided a volume-rendering routine so that, by choosing suitable x-ray linear attenuation coefficient thresholds, enamel (2.78 cm(-1) at 40 keV) and dentin (1.63 cm(-1) at 40 keV) surfaces could be viewed. The percentage area of enamel conserved was analyzed from these images. Coordinate metrology was used to produce color-coded images depicting the depth of preparation. The Kruskal-Wallis test was used to determine the statistical significance (alpha=.05) in the difference between the mean percentage area of enamel conserved in the 3 technique groups. The coordinate metrology and x-ray microtomography images were visually compared to study the correlation between overpreparation and dentin exposure. RESULTS: The Kruskal-Wallis test did not demonstrate significant difference (P=.07) between the 3 techniques in conserving enamel. However, the dimple technique showed a greater trend to retaining a larger mean percentage area of enamel (77.5% +/- 14.2) compared to depth-groove (50.1% +/- 17.5) and freehand (76.8% +/- 24.4) techniques. Preparation depth in the range of 0.4 to 0.6 mm was largely seen to be intraenamel, except in the cervical region. CONCLUSION: Within the limitations of this pilot study, the 3 different techniques tested did not differ significantly in conserving enamel.

Case report: removal of fractured endodontic posts with a sonic instrument.

Fracture of endodontic posts within the root canal system is one of the causes of failure to restore endodontically treated teeth. Various techniques, with varying degrees of success, have been proposed in the literature for the removal of fractured posts prior to re-restoring the tooth. This case report describes the use of a sonic device to dislodge and remove two fractured prefabricated metal endodontic posts from teeth UR1 and UL2. The reader is also introduced to a variety of post removal techniques available.