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1.
World J Clin Cases ; 11(25): 5870-5877, 2023 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-37727475

RESUMO

BACKGROUND: Young and middle-aged cancer patients in intensive care unit (ICU) often suffer from stress and pressure, causing huge physical and mental damage. Currently, there is few research on post-traumatic stress disorder (PTSD) among young and middle-aged cancer patients in ICU in China, and the psychological status of patients who have experienced both cancer development and ICU stay is still unclear. AIM: To explore the risk factors for PTSD in young and middle-aged patients with cancer in ICU. METHODS: Using convenient sampling method, we enrolled 150 young and middle-aged patients with cancer who were admitted to the ICU of our center during the period from July to December 2020. The general data of the patients and PTSD-related indicators were collected. The Impact of Event Scale-Revised (IES-R) was used for assessing PTSD one month after the discharge from the ICU. Binary Logistic regression analysis was performed to assess the independent risk factors for PTSD in these patients. RESULTS: Among these 150 patients, 32 (21.33%) were found to be with PTSD. Binary Logistic regression analysis revealed that factors significantly associated with PTSD among young and middle-aged patients with cancer in ICU included monthly income (OR = 0.24, P = 0.02), planned transfers (OR = 0.208, P = 0.019), and Acute Physiology and Chronic Health Evaluation (APACHE II) score (OR = 1.171, P = 0.003). CONCLUSION: The low monthly income, unplanned transfers, and increased APACHE II score are the risk factors for PTSD in young and middle-aged patients with cancer in ICU.

2.
Biomed Environ Sci ; 27(2): 111-7, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24625401

RESUMO

OBJECTIVE: To explore the role of HIV-1 tat gene variations in AIDS dementia complex (ADC) pathogenesis. METHODS: HIV-1 tat genes derived from peripheral spleen and central basal ganglia of an AIDS patient with ADC and an AIDS patient without ADC were cloned for sequence analysis. HIV-1 tat gene sequence alignment was performed by using CLUSTAL W and the phylogentic analysis was conducted by using Neighbor-joining with MEGA4 software. All tat genes were used to construct recombinant retroviral expressing vector MSCV-IRES-GFP/tat. The MSCV-IRES-GFP/tat was cotransfected into 293T cells with pCMV-VSV-G and pUMVC vectors to assemble the recombinant retrovirus. After infection of gliomas U87 cells with equal amount of the recombinant retrovirus, TNF-α, and IL-1ß concentrations in the supernatant of U87 cells were determined with ELISA. RESULTS: HIV-1 tat genes derived from peripheral spleen and central basal ganglia of the AIDS patient with ADC and the other one without ADC exhibited genetic variations. Tat variations and amino acid mutation sites existed mainly at Tat protein core functional area (38-47aa). All Tat proteins could induce U87 cells to produce TNF-α and IL-1ß, but the level of IL-1ß production was different among Tat proteins derived from the ADC patient's spleen, basal ganglia, and the non-ADC patient's spleen. The level of Tat proteins derived from the ADC patient's spleen, basal ganglia, and the non-ADC patient's spleen were obviously higher than that from the non-ADC patient's basal ganglia. CONCLUSION: Tat protein core functional area (38-47aa) may serve as the key area of enhancing the secretion of IL-1ß. This may be related with the neurotoxicity of HIV-1 Tat.


Assuntos
Complexo AIDS Demência/metabolismo , Complexo AIDS Demência/virologia , Genes tat , HIV-1/patogenicidade , Interleucina-1beta/metabolismo , Neuroglia/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , Produtos do Gene tat do Vírus da Imunodeficiência Humana/fisiologia , Complexo AIDS Demência/patologia , Adulto , Sequência de Aminoácidos , Gânglios da Base/virologia , Linhagem Celular Tumoral , Regulação Viral da Expressão Gênica , HIV-1/genética , Humanos , Interleucina-1beta/biossíntese , Interleucina-1beta/genética , Pessoa de Meia-Idade , Dados de Sequência Molecular , Neuroglia/patologia , Baço/virologia , Fator de Necrose Tumoral alfa/biossíntese , Fator de Necrose Tumoral alfa/genética , Produtos do Gene tat do Vírus da Imunodeficiência Humana/genética
3.
Artigo em Chinês | MEDLINE | ID: mdl-23855121

RESUMO

OBJECTIVE: To study the genetic diversity of HIV-1 nef genes from a patient with AIDS dementia complex(ADC) , so as to research the amino acid variability and the pathogenesis of ADC. METHODS: The nef gene was amplified with PCR from genomic DNA which was extracted from spleen and different brain tissues(basal ganglia, frontal gray matter, meninges, temporal lobe)of a patient who died of ADC. PCR products were cloned into the pMD19-T vector, after transformation and selection by ampicillin and blue/white spotting. Five of positive clones were sequenced and confirmed with BLAST. HIV-1 nef sequences were processed with BioEdit and MEGA4 to do Neighbor-Joining tree, p-Distances, and values of ds/dn. RESULTS: The samples were all identified as HIV-1 B and genetic variation exists in HIV-1 nef gene isolated from different tissues compared with HXB2. In addition,part of the changes were different between periphery and brain. CONCLUSION: Variations exist in the HIV-1 nef gene extracted from the ADC patient and the variations from peripheral and central nerve tissues were different,these variations may change the function of Nef,and it needs more research.


Assuntos
Complexo AIDS Demência/virologia , Infecções por HIV/virologia , HIV-1/genética , Produtos do Gene nef do Vírus da Imunodeficiência Humana/genética , Adulto , DNA Viral/genética , Variação Genética , Humanos , Masculino
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