Draft Genome Sequences of Three Escherichia coli Strains Investigated for the Effects of Lysogeny on Niche Diversification.

During the course of investigating the effects of lysogeny on niche diversification of Escherichia coli, we used the temperate phages induced from one E. coli strain to infect another and created an isogenic lysogen of the latter. The draft genome sequences of the three E. coli strains are reported herein.

Draft Genome Sequence of Escherichia coli E1728 Isolated from Marine Sediment in Hong Kong.

Recent findings of Escherichia coli persisting autochthonously in environmental matrices outside animal bodies have revealed largely unknown facets of the lifestyle and ecophysiology of the species that have yet to be explored. Here, we report the draft genome sequence of E. coli E1728 isolated from marine sediment.

Host-specific 16S rRNA gene markers of Bacteroidales for source tracking of fecal pollution in the subtropical coastal seawater of Hong Kong.

This study investigated the diversity of Bacteroidales communities in the feces of eight host species in Hong Kong (subtropical Asia), including human (in the form of sewage), cow, pig, horse, cat, dog, rabbit and rat. The analysis of terminal restriction fragment length polymorphism (TRFLP) in the 16S rRNA genes revealed significant differences in Bacteroidales communities among all host species, with the exception of dog and cat. Manual examination of TRFLP profiles resulted in six terminal restriction fragments (TRFs) that were potentially specific to the sewage (one TRF), cow (three TRFs) or pig (two TRFs) samples. All six TRFs were (1) present in 100% of the samples of the respective target host, (2) absent in other hosts or present only in low frequency and low intensity, and (3) verified for sizes using in silico digestion of DNA sequences in clone libraries. The six TRFs could reliably indicate the source of fecal contaminations in natural seawater amended with sewage, cow or pig fecal samples. In field tests conducted for two polluted and one unpolluted coastal site, the sewage-specific TRF was detected in all seawater samples of the sites known to be impacted by raw and treated sewage. However, only two of three cow-specific TRFs were detected for the two polluted sites, which also received fecal input from feral cows. No pig-specific TRF was detected, although one of the coastal sites was chronically polluted by pig farm run-offs. Nevertheless, the total absence of the six potentially host-specific TRFs in the seawater of an unpolluted site demonstrated the specificity of the TRFs as gene markers in indicating actual pollution.

[Minimal invasive surgery in female urinary incontinence: TVT-O]. / Cirugía mínimamente invasiva en la incontinencia urinaria femenina: TVT-O.

OBJECTIVE: We presented our experience in the application of Tvt-O, a surgical a new technic for SUI. METHOD: Between April and December 2004, 96 patients underwent Tvt-O surgery, in Uroginecology and Vaginal Surgery Unit of the Gynecology and Obstetrics Department, Las Condes Clinic. Median age was 54 years old, weight 65 kgs. Urodinamia test: SUI II 80 cases, SUI III 4, SUI 0 in 3, and mixed urinary incontinence 9. Gynecare TVT Obturator System technique was used, Ethicon Johnson & Johnson. It has three specific instruments: helical passers, plastic tubes fixed to mesh of prolene, and a guide for the introduction of the needle. RESULTS: Media time was 7 minutes (4 to 15). Other gynecological surgery was associated in 77 (80%). Complications did not appear during the surgical act. No injury to bladder and urethra was observed. We observed in immediate postoperative period only one complication (1.04%). It was urinary retention. One complication appeared (1.04%) in remote postoperative period, corresponding polypropylene tape exposition in the anterior vaginal wall. SUI solution was obtained in 100% of patients. The medium time of observation is 6 months, 51 cases have more than 6 months of observation. DISCUSSION: Tvt-O is a surgical SUI correction technique with promissory results. Like the other types of TOT, their effectiveness will have in the long term to be evaluated.

Cirugía mínimamente invasiva en la incontinencia urinaria femenina: TVT-O / Minimal invasive surgery in female urinary incontinence: TVT-O

Objetivo: Presentamos nuestra experiencia en la aplicación de TVT-O, en la corrección quirúrgica de la IOE. Método: Entre abril y diciembre 2004, se realizaron 96 TVT-O a pacientes de la Unidad de Uroginecología y Cirugía Vaginal, del Departamento de Ginecología y Obstetricia de la Clínica Las Condes. Mediana de edad 54 años, peso 65 kg. Test de urodinamia: IOE grado II 80 casos, IOE grado III 4, IOE grado 0 3, e IOM 9. Se utilizó el Sistema Gynecare TVT Obturador de Ethicon, Johnson & Johnson. Resultados: Mediana tiempo operatorio TVT-O: 7 minutos. Se asoció alguna cirugía ginecológica en 77. No se presentaron complicaciones durante el acto quirúrgico. No se observó daño de vejiga ni vías urinarias. Durante el postoperatorio inmediato se presentó sólo una complicación (1,04%) correspondiendo a retención urinaria, por sobrecorrección. Durante el postoperatorio tardío una complicación (1,04%), exposición de la cinta de polipropileno en la mucosa de la pared vaginal anterior. Se obtuvo solución de la incontinencia de orina de esfuerzo en las 96 pacientes. La mediana de seguimiento 6 meses, 51 casos con seguimiento mayor a 6 meses. Conclusiones: TVT-O es una técnica quirúrgica para el tratamiento de la IOE, con resultados muy promisorios. Al igual que los otros tipos de TOT, su eficacia deberá ser evaluada a largo plazo

Objective: We presented our experience in the application of Tvt-O, a surgical a new technic for SUI. Method: Between April and December 2004, 96 patients underwent Tvt-O surgery, in Uroginecology and Vaginal Surgery Unit of the Gynecology and Obstetrics Department, Las Condes Clinic. Median age was 54 years old, weight 65 kgs. Urodinamia test: SUI II 80 cases, SUI III 4, SUI 0 in 3, and mixed urinary incontinence 9. Gynecare TVT Obturator System technique was used, Ethicon Johnson & Johnson. It has three specific instruments: helical passers, plastic tubes fixed to mesh of prolene, and a guide for the introduction of the needle. Results: Media time was 7 minutes (4 to 15). Other gynecological surgery was associated in 77 (80%). Complications did not appear during the surgical act. No injury to bladder and urethra was observed. We observed in immediate postoperative period only one complication (1.04%). It was urinary retention. One complication appeared (1.04%) in remote postoperative period, corresponding polypropylene tape exposition in the anterior vaginal wall. SUI solution was obtained in 100% of patients. The medium time of observation is 6 months, 51 cases have more than 6 months of observation. Discussion: Tvt-O is a surgical SUI correction technique with promissory results. Like the other types of TOT, their effectiveness will have in the long term to be evaluated