Neurotoxic effects of carbamazepine on the mosquitofish Gambusia affinis.

In recent years, the presence of pharmaceuticals in the aquatic environment has gained a significant attention. Carbamazepine, a commonly prescribed antiepileptic drug, has been consistently found in aquatic environments at concentrations ranging from nanograms to micrograms, raising concerns about its potential negative impacts on aquatic organisms. The study examined the acute and chronic neurotoxic effects of environmentally relevant and sublethal concentrations of carbamazepine in the mosquitofish Gambusia affinis. After a 96-hour exposure period, the median lethal concentration (LC50) of carbamazepine for G. affinis was determined as 24 mg L - 1. For the current study, sublethal concentrations i.e., one-tenth (2.4 mg L - 1) and one-fifth (4.8 mg L - 1) of the LC50 value were chosen for assessing the neurotoxic effects along with the environmentally relevant concentration (13 ng L - 1). The research findings indicated that carbamazepine had a disruptive impact on the typical growth and behavior of the fish. During the acute exposure phase, physical deformities were observed in the fish, resulting in neonatal and postneonatal fatalities. Furthermore, the neurotoxic effects of carbamazepine were clearly demonstrated through alterations in various neurological parameters, including acetylcholinesterase, dopamine, gamma-aminobutyric acid, serotonin, monoamine oxidase, 5-hydroxyindole acetic acid, adrenaline, and nor-adrenaline. These findings raise concerns about the survival of fish populations in their natural environment.

Exposure to the plasticizer dibutyl phthalate causes oxidative stress and neurotoxicity in brain tissue.

The phthalate ester, dibutyl phthalate (DBP), is one of the endocrine-disrupting chemicals detected in various aquatic environments. Previous research has found multiple toxic effects of DBP in aquatic organisms; however, the neurotoxic effects of the compound are surprisingly scanty. The purpose of this study was aimed to evaluate the role of oxidative stress in the induction of neurotoxicity in the brain tissue of the fish Pseudetroplus maculatus. The fish were exposed to the sublethal concentration of DBP (200 µg L-1) for 1, 4, 7, and 15 days along with control and vehicle control groups. The induction of oxidative stress in the brain subcellular fractions was proved by alterations in the activities of superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase along with the reduction in the total antioxidant capacity. Meanwhile, the levels of hydrogen peroxide and lipid peroxidation were increased. Neurotransmitters such as acetylcholine, dopamine, adrenaline, noradrenaline, and serotonin were altered in all subcellular fractions suggesting the disruption of the neurotransmitter system in the fish brain. These results indicate that DBP induces oxidative stress, which correlates with neurotoxicity in Pseudetroplus maculatus brain tissue.

A comparative study on the sensitivity of selected freshwater fishes against triclosan exposure.

Triclosan, a broad-spectrum antimicrobial agent, which is widely used in several pharmaceutical and personal care products, has been known to pose adverse impacts on various groups of aquatic organisms, including fish. The present study aimed to compare the sensitivity of five selected freshwater fishes toward triclosan exposure. The acute toxicity or median lethal concentration (LC50-96 h) of triclosan determined using probit analysis was 1.76 mg L-1 in Anabas testudineus, 448 µg L-1 in Danio rerio, 700 µg L-1 in Gambusia affinis, 1.35 mg L-1 in Oreochromis mossambicus, and 750 µg L-1 in Pseudetroplus maculatus. Based on the length-weight relationship, the lowest condition factor, or K value, was recorded in Danio rerio (0.931) while the highest value was observed in Anabas testudineus (2.343) indicating negative allometric growth. Similarly, the weight of fishes and gill weight declined significantly (p < 0.05) in their respective LC50-96 h groups with concomitant alterations in the rate of oxygen consumption denoting respiratory distress. Triclosan exposure also resulted in the modification of fish behavior and histomorphological damage in gill tissues. The alterations in all parameters tested were more prominent in Danio rerio and thus considered the most sensitive species, while Anabas testudineus comparatively tolerated the toxicant to some extent. The study provides the baseline data that variation in the length-weight relationship is one of the major factors that influences the toxic effects of triclosan on the selected freshwater fishes.

Acute and sublethal effects of acrylamide on the freshwater fish Anabas testudineus (Bloch, 1792).

Acrylamide, a synthetic compound, has a wide range of industrial applications that find multiple ways to reach aquatic ecosystem. The median lethal concentration of acrylamide determined using probit analysis in the fish Anabas testudineus was 132 µg L-1 concentration together with altered behavioral patterns. Hematological and antioxidant status was evaluated at a sublethal concentration (one-tenth of LC50-96 h), i.e., 13.2 µg L-1 concentration for 24, 48, 72, and 96 h. A reduction in erythrocytes count, hemoglobin content, and packed cell volume with a significant (P < 0.05) increase in leukocyte counts and differential counts were observed. Erythrocyte indices like mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) increased, whereas the mean corpuscular hemoglobin concentration (MCHC) showed a significant (P < 0.05) decrease when compared with control groups. The activities of superoxide dismutase, catalase, and glutathione reductase in gill tissues showed significant (P < 0.05) reduction, whereas the levels of hydrogen peroxide and lipid peroxidation increased significantly (P < 0.05) indicating oxidative stress. The findings suggest that acrylamide at sublethal concentration caused alteration in hematological parameters and induced oxidative stress in gill tissue of the fish A. testudineus. Hence, restrictions on the use of acrylamide in food and industrial products are recommended since humans are the direct consumer of fish products.

Possible role of C60 fullerene in the induction of reproductive toxicity in the freshwater fish, Anabas testudineus (Bloch, 1792).

In recent years, the impact of ultrafine nanomaterials on the aquatic organisms and their ecosystems contributed much concern due to their abundance in environment. Several toxicity studies have reported that nanoparticles induced reproductive stress and resulted in reproductive impairment of fishes. The present study was aimed to investigate the stress-induced toxicity of C60 fullerene nanomaterial on various reproductive parameters of the freshwater fish, Anabas testudineus. Fish were exposed to two sublethal concentrations of fullerene C60, one-tenth (5 mg/L) and one-fifth (10 mg/L) of LC50-96 h, for 4, 7, 15, 30, and 60-day durations. At the end of exposure period, the activities of steroidogenic enzymes, 3ß-, and 17ß-hydroxysteroid dehydrogenase decreased in the testis and ovary thereby indicated that the nanomaterial affected gonadal steroidogenesis. The level of serum testosterone decreased significantly (p < 0.05) in male whereas the level of estradiol showed significant (p < 0.05) reduction in female fish with significant (p < 0.05) increase in the level of serum cortisol in both sexes in concentration- and time-dependent manner. The analysis of the levels of alkali-labile phosphates, plasma calcium, and total protein showed significant (p < 0.05) reduction in female fish without significant changes in male fish, and this could be due to the antiestrogenic action of fullerene C60 nanomaterial. The activity of aromatase enzyme decreased significantly (p < 0.05) in the ovary and brain of female fish, and the decline in the enzyme activity was prominent only in the brain tissue of male fish. The present results suggested that the stress-induced by fullerene C60 exposure provoked reproductive toxicity in the fish, Anabas testudineus.

Cytogenotoxic effects of fullerene C60 in the freshwater teleostean fish, Anabas testudineus (Bloch, 1792).

In recent years, carbon nanomaterials, including fullerene C60 is regarded as the building block in nanotechnology because of its widespread use in medicine, industry, cosmetics and commercial products. Despite the special properties, several reports have raised public health concerns due to the unknown and practically unexplored toxic effects of nanomaterials. However, there have been relatively few studies regarding the genotoxic responses of fullerene C60in vivo. Genotoxic effects of DMSO-solublized C60 nanomaterial suspension at sublethal concentrations (5 and 10 mg/L) were investigated on adult freshwater fish, Anabas testudineus using micronucleus and comet assays. An assessment of micronucleus induction showed severe cytoplasmic and nuclear abnormalities in erythrocytes, gill and liver cells. Abnormalities in cytoplasm were identified as formation of sticky cells, vacuolated cytoplasm, cytoplasmic degeneration, echinocyte, acanthocyte, anisochromatic cells and abnormal erythrocyte membrane. The nuclear abnormalities included micronucleus, binucleated cells, nuclear buds, irregular nucleus, vacuolated, notched and serrated nucleus in the erythrocytes compared to the control groups. Similarly, significant increase (P < 0.05) in micronucleus frequencies were observed in gill and liver cells. The high frequency of micronucleus was observed in the gill cells followed by liver and erythrocytes, respectively, at both sublethal concentrations, and the severity was duration and concentration-dependent. In comet assay, significant increase (P < 0.05) in DNA damage was observed using the comet parameter, percent tail DNA. The highest level of comet damage with grade 3 was observed in blood, gill and liver cells on increase in duration and concentration when compared to the respective control groups. Thus the results revealed that fullerene C60 nanomaterials may pose risk to aquatic organisms, especially fish, by the induction of genotoxicity. Further studies are warranted to provide new insights on the mechanisms and consequences of C60 nanomaterials interactions with biological membranes.