Phylogeography, origin and population structure of the self-fertile emerging plant pathogen Phytophthora pseudosyringae.

Phytophthora pseudosyringae is a self-fertile pathogen of woody plants, particularly associated with tree species from the genera Fagus, Notholithocarpus, Nothofagus and Quercus, which is found across Europe and in parts of North America and Chile. It can behave as a soil pathogen infecting roots and the stem collar region, as well as an aerial pathogen infecting leaves, twigs and stem barks, causing particular damage in the United Kingdom and western North America. The population structure, migration and potential outcrossing of a worldwide collection of isolates were investigated using genotyping-by-sequencing. Coalescent-based migration analysis revealed that the North American population originated from Europe. Historical gene flow has occurred between the continents in both directions to some extent, yet contemporary migration is overwhelmingly from Europe to North America. Two broad population clusters dominate the global population of the pathogen, with a subgroup derived from one of the main clusters found only in western North America. Index of association and network analyses indicate an influential level of outcrossing has occurred in this preferentially inbreeding, homothallic oomycete. Outcrossing between the two main population clusters has created distinct subgroups of admixed individuals that are, however, less common than the main population clusters. Differences in life history traits between the two main population clusters should be further investigated together with virulence and host range tests to evaluate the risk each population poses to natural environments worldwide.

A new hybrid of Phytophthora from Southeast Europe.

During an investigation of rivers in Bulgaria, an isolate of Phytophthora (RLKam2016/61c) was recovered and initially identified as Phytophthora sansomeana based on internal transcribed spacer region (ITS) sequence data. However, the sequencing of the mitochondrial cytochrome c oxidase subunit I (cox1) gene revealed high similarity to Phytophthora sp. kelmania, and sequencing of other nuclear regions (ß-tubulin [Btub] and translation elongation factor 1-alpha [tef1]) revealed a significant number of polymorphisms, indicating a possible hybridization event. Additional cloning and sequencing of the nuclear ITS and Btub regions showed the presence of two distinct groups of alleles, one of which was highly similar to P. sansomeana, whereas the other was similar to a species complex that includes Phytophthora sp. kelmania. Therefore, the new hybrid was named Phytophthora × sansomeana. It is characterized by fast growth on V8 juice agar (V8A) and carrot agar (CA), moderate aerial mycelium with radiate pattern of the colonies and relatively slower growth rate on malt extract agar (MEA) and potato dextrose agar (PDA), and petaloid to rosaceous pattern of the colonies with fluffy aerial mycelium. The optimum growth temperature for P. × sansomeana was at 25 C, with an average growth rate of 9 mm per day. Abundant sporangium formation of the isolate in spring water was observed, but the hybrid was sterile in culture. Pathogenicity analyses of the hybrid were conducted in comparison with the most closely related subclade 8a species from our collection, P. pseudocryptogea. The inhibition effect on the root growth of young seedlings of two legumes, common pea and vetch, as well as on cuttings of the ornamental plant coleus induced by both phytopathogens was significant. No effect of either the new hybrid or P. pseudocryptogea on the growth of maize seedlings was observed.

Oxidation of oxymyoglobin by poplar plastocyanins a and b.

Oxidation of oxymyoglobin [MbO2(Fe2+)] by isoplastocyanins a (PCa) and b (PCb) was experimentally investigated and the corresponding redox reaction was modeled using the physicochemical parameters of the isoforms to study the effect of the dimorphism. The kinetic curve of oxidation of MbO2 (Fe2+) by oxidized PCa [PCa(Cu2+)] and PCb [PCb(Cu2+)] and the pH-dependence of the rate constant kI were determined. In the range of pH 4.8-9.0, PCb reacts with higher k1, compared with PCa. For example, at pH 7.0, k1(PCb) = 4 x 10(2) M(-1)s(-1), whereas k1(PCa)= 2 x 10(2) M(-1)S(-1). The observed values of deltaE(0) for the reaction pairs Mb-PCa and Mb-PCb were -304 mV and -319 mV, respectively. The effect of the ionic strength (mu) on the rate of the electron transfer was also studied. It was found that: (i) the net charge Z1 of PCa and PCb fully corresponds to that calculated by their primary structures and Z2 of Mb corresponds to that calculated by its titration curve; (ii) the In k as function of mean square of mu was similar for both PCa and PCb; (iii) the curve of the reaction PCb <----(e(-1)) Mb (pH 7.0) wasshifted towards higher values of k, in agreement with the larger net negative charge of PCb; and (iv) the character of the electrostatic interactions remained unchanged by a replacement of PCa by PCb and by the change of pH from 7.0 to 4.8.

Some physicochemical peculiarities of poplar plastocyanins a and b.

The redox potentials of poplar plastocyanins a and b (PCa, PCb) were determined by spectrophotometric titrations of their reduced forms with [Fe(CN)6]3-. It was found that the two isoforms have the following millimolar extinction coefficients epsilon597 equilibrium constants Keq of one-electron exchange with [Fe(CN)6]4-/[Fe(CN)6]3-, and standard electron potentials E0: PCa: epsilon597 = (4.72 +/- 0.08) mM(-1) cm(-1), Keq = 0.133 +/- 0.009, E0' = (354 +/- 11) mV; PCb: epsilon597 = (5.23 +/- 0.16) mM(-1) cm(-1), Keq = 0.175 +/- 0.010, E0' = (363 +/- 12) mV. The pH dependence of the redox potential of PCb was studied too. It was found, that the value of E0' for PCb is constant in the pH range 6.5-9.5, but decreases in the range 4.8-6.5. On the whole, the dependence resembles that of PC from some well-known plant species, including poplar PCa. The changes of E0' in the pH-dependent region for poplar PCb, however, are smaller and are 13 mV per pH unit, whereas in the other well-known plant species the changes are about 50-60 mV per pH unit. It has been assumed that the weaker pH dependence of EO' of PCb accounts for some structural differences between PCa and PCb.