RESUMO
Tribromsalan can be quantitatively measured in whole blood and urine by a technique involving extraction with ethyl acetate, treatment with silica gel, separation by TLC, and quantitative measurement by fluorescent spectrophotometry. This method has a sensitivity down to 125 ng (25 ppb in 5.0 ml of sample) of free tribromsalan and shows an average 90% recovery of tribromsalan in blood and urine with standard deviations of 9.7 and 7.4%, respectively.
Assuntos
Desinfetantes/análise , Salicilamidas/análise , Animais , Cromatografia em Camada Fina , Desinfetantes/sangue , Desinfetantes/urina , Humanos , Concentração de Íons de Hidrogênio , Métodos , Coelhos , Salicilamidas/sangue , Salicilamidas/urina , Salicilanilidas , Espectrometria de FluorescênciaRESUMO
A method is presented for quantitatively determining triclocarban in blood. Triclocarban is extracted from blood with ether, isolated by TLC, and measured through its UV absorption at 265 nm in methanol. This method is sensitive to 250 ng (50 ppb in 5 ml of blood) of free triclocarban with a relative standard deviation of 5.2%, correlated with a radiotracer analysis of 14C-labeled triclocarban. It has been applied successfully to the analysis of triclocarban in human and rabbit blood.
