SARS-CoV-2 and SARS-CoV Spike-RBD Structure and Receptor Binding Comparison and Potential Implications on Neutralizing Antibody and Vaccine Development

SARS-CoV-2 and SARS-CoV share a common human receptor ACE2. Protein-protein interaction structure modeling indicates that spike-RBD of the two viruses also has similar overall binding conformation and binding free energy to ACE2. In vitro assays using recombinant ACE2 proteins and ACE2 expressing cells confirmed the two coronaviruses similar binding affinities to ACE2. The above studies provide experimental supporting evidences and possible explanation for the high transmissibility observed in the SARS-CoV-2 outbreak. Potent ACE2-blocking SARS-CoV neutralizing antibodies showed limited cross-binding and neutralizing activities to SARS-CoV-2. ACE2-non-blocking SARS-CoV RBD antibodies, though with weaker neutralizing activities against SARS-CoV, showed positive cross-neutralizing activities to SARS-CoV-2 with an unknown mechanism. These findings suggest a trade-off between the efficacy and spectrum for therapeutic antibodies to different coronaviruses, and hence highlight the possibilities and challenges in developing broadly protecting antibodies and vaccines against SARS-CoV-2 and its future mutants.

The analysis of depression,anxiety and sleep disorder in earthquake-related injuries / 中华内科杂志

During the psychological intervention for patients injured and patients with internal disease in earthquake in Sichuan,psychological assessment of anxiety,depression and sleep disorder was carried for them by conversation and observation.By analysis,it showed that the patients suffering from depression and anxiety was 41.8% and sleep disorder WaS 73%.Further more,sleep disorder was significantly correlated with depression and anxiety.

The Defense Mechanism and the Changes after the Treatment of Patients with Obsessive-Compulsive Disorder / 中国心理卫生杂志

Objective: To explore the defense mechanism of patients with Obsessive-Compulsive Disorder(OCD)and to measure the changes in defense mechanisms after the treatment. Methods: Before and after 10- week psychotherapy , 26 patients with OCD were assessed with the Defense Style Questionnaire(DSQ)and Yale-Brown Obsessive-Compulsive Scale(Y-BOCS); DSQ were also used to test 26 normal subjects. Results: (1)The scores of immature mechanism factor (4.5?0.8)were significantly higher in OCD patients than in normal(3.0?0.5),the scores of mature mechanism factor(5.0?0.8)were significantly lower in OCD patients than in normal (5.7?0.7); (2)After 10- week psychotherapy the scores of immature mechanism factor(4.0?0.9)were significantly lower than before (4.5?1.0),the scores of mature mechanism factor(5.7?0.7)were significantly higher than before (5.0?0.9).Conclusion: OCD patients often use immature mechanism, but after psychotherapy, they can use more mature mechanism.