Cyanuric chloride has no genotoxic and mutagenic properties in bacteria and bone marrow cells.

Three short-term tests were used; Salmonella/microsome assay, in vivo sister chromatid exchange assay (SCE) and micronucleus assay to evaluate mutagenic and genotoxic properties of 2,4,6-trichlorotriazine; cyanuric chloride. Mutagenicity assays were carried out using the standard top agar overplay plate assay described by Maron and Ames (9). Tester strains TA97a, TA98, TA100 and TA102 were used. Compound was dissolved in 0.1 ml of DMSO and doses of 1, 10, 100 and 500 micrograms/plate were tested in the absence and in the presence of the S9-mix. From the results obtained it appeared that incubation of the test substance with the bacteria did not increase the number of His+ revertants with any of the strains of S. typhimurium, either in the absence or in the presence of the S9-mix. At the high dose level used i.e. 100 and 500 micrograms/plate, the test substance appeared to be slightly toxic for strain TA 97a (in the absence of the S9-mix), as was seen from a diminished number of revertant colonies. The SCE test was performed according to the GENE-TOX programme. No significant increase was noted in the incidence of SCE in the groups treated with all tested doses of cyanuric chloride. Thus, in this test cyanuric chloride did not induce chromosomal damage resulting in SCE formation in bone marrow cells of mice. The micronucleus assay in vivo was performed on mice bone marrow cells. The incidence of micronucleated polychromatic erythrocytes after administration of all doses of cyanuric chloride used were not statistically different (p > 0.05) as compared to negative controls.(ABSTRACT TRUNCATED AT 250 WORDS)

Genotoxicity assessment of Rokanol B2 and Rokamid R1.

Two surface-active compounds, Rokanol B2 and Rokamid R1, were examined with three types of screening tests: 1. standard Ames test in vitro using S. typhimurium TA97a, TA98, TA100 and TA102 strains; 2. micronucleus test in vivo; 3. sister chromatid exchange (SCE) on bone marrow cells of Balb C mice. Negative results of the testing, in terms of the effects of activity of both the technical preparations on S. typhimurium cells and bone marrow of Balb C mice, were found for both tested chemicals.

[Methods for the evaluation of the mutagenic and genotoxic properties of chemical compounds. III. The sister chromatid exchange test in vivo (an abbreviated method)]. / Metodyki stosowane do oceny wlasciwosci mutagennych i genotoksycznych zwiazków chemicznych. Czesc III. Test wymian chromatyd siostrzanych (SCE) in vivo.

The principle and experimental procedure of the in vivo sister chromatid exchange (SCE) test applied in the screening of chemical compounds for their mutagenic properties are presented. The objective of this assay is to evaluate the ability of a test chemical to induce SCE in bone marrow cells of laboratory rodents. Animals are exposed to test substances by appropriate means and are sacrificed at sequential intervals. Chromosome preparations from bone marrow cells of the exposed animals are stained and examined for the presence sister chromatid exchange. The criterion of mutagenic effect of the test chemical is the dose-related increase in SCE frequency and/or statistically significant and reproducible positive response (beyond the level of the control group) for at least one dose of the test chemical.