RESUMO
Numerous isolates of both subspecies of Staphylococcus cohnii were found in the environment of the intensive-care unit of a pediatric hospital. These isolates carried in their cells many plasmids, up to fourteen, of a wide range of sizes (< 2 to > 56 kb). Striking was the occurrence of large plasmids not very common in staphylococci. These were present in > 80% of S. cohnii isolates. Fifty-two different plasmid profiles were found in 79 investigated isolates belonging to S. cohnii ssp. cohnii and S. cohnii ssp. urealyticus. Isolates similar in plasmid profiles were grouped in antibiotic-resistance clusters established for 9 antibiotics (gentamicin, ciprofloxacin, clindamycin, erythromycin, tetracycline, chloramphenicol, mupirocin, trimethoprim-sulfamethoxazole, vancomycin) using the method of unweighted pair group mathematical averages (UPGMA). Many isolates were multiresistant to antibiotics and produced bacteriocins.
Assuntos
Hospitais Pediátricos , Unidades de Terapia Intensiva , Plasmídeos , Staphylococcus/genética , Staphylococcus/isolamento & purificação , Antibacterianos/farmacologia , Técnicas de Tipagem Bacteriana , Bacteriocinas/biossíntese , Cloranfenicol/farmacologia , Ciprofloxacina/farmacologia , Clindamicina/farmacologia , Impressões Digitais de DNA , DNA Bacteriano/análise , DNA Bacteriano/isolamento & purificação , Farmacorresistência Bacteriana/genética , Farmacorresistência Bacteriana Múltipla/genética , Eritromicina/farmacologia , Gentamicinas/farmacologia , Testes de Sensibilidade Microbiana , Epidemiologia Molecular , Mupirocina/farmacologia , Polônia , Staphylococcus/classificação , Staphylococcus/efeitos dos fármacos , Tetraciclina/farmacologia , Combinação Trimetoprima e Sulfametoxazol/farmacologia , Vancomicina/farmacologiaRESUMO
In this study, we found Lewis X (Le(x)) determinants on 68% of Helicobacter pylori isolates from patients with chronic gastroduodenal diseases. Anti-Le(x) IgG were detected more frequently in the sera from dyspeptic children and adults (45 and 46%), with or without proved (culture) H. pylori infection, than in the sera from healthy individuals (14% and 25%). In contrast, the prevalence of anti-Le(x) IgM was higher in the groups of healthy individuals than in the groups of dyspeptic patients. Moreover, anti-Le(x) monoclonal antibody of IgM class enhanced the uptake of Le(x)(+) but not Le(x)(-) H. pylori isolates by phagocytes. In the sera from some dyspeptic patients, we detected Le(x)-anti-Le(x) IgG immune complexes (Le(x) ICs). There was a great difference between children and adults as regards the presence of Le(x) ICs. The immune complexes were found in the sera from nine out of 29 (27%) H. pylori-infected and three out of eight (37%) uninfected adult dyspeptic patients. In comparison, Le(x)-anti-Le(x) IgG ICs were detected only for two out of 18 (11%) H. pylori-infected children. Le(x) ICs were not found in the sera from healthy individuals. Our results suggest that anti-Le(x) IgM may play a protective role in H. pylori infections. In contrast, anti-Le(x) IgG and particularly Le(x)-anti-Le(x) IgG ICs might contribute to the pathogenesis of chronic H. pylori infections.
Assuntos
Antígenos de Bactérias , Infecções por Helicobacter/imunologia , Helicobacter pylori/imunologia , Antígenos CD15/imunologia , Úlcera Péptica/imunologia , Adolescente , Adulto , Idoso , Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/imunologia , Criança , Dispepsia/imunologia , Infecções por Helicobacter/complicações , Helicobacter pylori/metabolismo , Humanos , Imunoglobulina G/sangue , Antígenos CD15/biossíntese , Pessoa de Meia-Idade , Úlcera Péptica/complicações , Fagocitose/imunologiaRESUMO
Three Proteus species P. vulgaris, P. mirabilis and P. penneri have been characterized by repetitive sequence-based PCR. Four families of repetitive sequence based primers REP, ERIC, BOXA1R and BOXA2R, give specific patterns for each Proteus species. Species differentiation was best afforded using BOXA2R for detection of P. mirabilis, either REP-Dt or BOXA1R primers for detection of P. penneri and ERIC primer pair for P. vulgaris.
