Taxonomic revision and cladistic analysis of the tarantula genera Tapinauchenius Ausserer, 1871, Psalmopoeus Pocock, 1985, and Amazonius n. gen. (Theraphosidae, Psalmopoeinae).

The genera Psalmopoeus Pocock, 1895, Tapinauchenius Ausserer, 1871 and Pseudoclamoris Hsser, 2018 are revised and cladistics analyses carried out including most species of these genera. In order to test the monophyly of Aviculariinae and Psalmopoeinae, species of all genera in these two subfamilies were included, as well as of Harpactirinae, Selenocosmiinae, Theraphosinae, Stromatopelmatinae, Ischnocolinae, Schismatothelinae, Poecilotherinae, and a barychelid was used to root the cladogram. The matrix with 50 terminal taxa, 1 continuous and 85 discrete characters was analysed with TNT 1.5. The results show a monophyletic Psalmopoeinae as sister group of Aviculariinae. Psalmopoeus comprises 9 species: Psalmopoeus cambridgei Pocock, 1895 (type species), Psalmopoeus ecclesiasticus Pocock, 1903, Psalmopoeus emeraldus Pocock, 1903, Psalmopoeus irminia Saager, 1994, Psalmopoeus langenbucheri Schmidt, Bullmer Thierer-Lutz, 2006, Psalmopoeus plantaris Pocock, 1903, Psalmopoeus pulcher Petrunkevitch, 1925, Psalmopoeus reduncus (Karsch, 1880), and Psalmopoeus victori Mendoza, 2014. Psalmopoeus intermedius Chamberlin, 1940 is considered a junior synonym of P. reduncus. Psalmopoeus copanensis Gabriel Sherwood, 2020, P. sandersoni Gabriel Sherwood, 2020 and P. petenensis Gabriel Sherwood, 2020 are considered junior synonyms of P. victori. Psalmopoeus maya Witt, 1996 is considered nomen nudum. Tapinauchenius comprises 8 species: Tapinauchenius plumipes (C. L. Koch, 1842) (type species), Tapinauchenius sanctivincenti (Walckenaer, 1837), Tapinauchenius latipes L. Koch, 1875, Tapinauchenius brunneus Schmidt, 1995, Tapinauchenius cupreus Schmidt Bauer, 1996, Tapinauchenius polybotes Hsser, 2018, Tapinauchenius rasti Hsser, 2018, and T. gretae n. sp. The female of T. brunneus is described for first time. Tapinauchenius violaceus (Mello-Leito, 1930), T. purpureus Schmidt, 1995, T. concolor (Caporiaccco, 1947), and T. gigas Caporiacco, 1954 are considered junior synonyms of T. plumipes. With the synonymy of T. gigas (type species of Pseudoclamoris Hsser, 2018), it was necessary to describe a new genus for the two species formerly included in it. Thus, the new genus Amazonius n. gen. is erected to include A. elenae (Schmidt, 1994) n. comb., A. burgessi (Hsser, 2018) n. comb. as well as two new species A. giovaninii n. gen. n. sp. and A. germani n. gen. n. sp. A discussion on the relationship of Psalmopoeinae and Aviculariinae and maps with the distributions of all species are provided.

Taxonomic revision and cladistic analysis of the tarantula genera Tapinauchenius Ausserer, 1871, Psalmopoeus Pocock, 1985, and Amazonius n. gen. (Theraphosidae, Psalmopoeinae)

The genera Psalmopoeus Pocock, 1895, Tapinauchenius Ausserer, 1871 and Pseudoclamoris Hüsser, 2018 are revised and cladistics analyses carried out including most species of these genera. In order to test the monophyly of Aviculariinae and Psalmopoeinae, species of all genera in these two subfamilies were included, as well as of Harpactirinae, Selenocosmiinae, Theraphosinae, Stromatopelmatinae, Ischnocolinae, Schismatothelinae, Poecilotherinae, and a barychelid was used to root the cladogram. The matrix with 50 terminal taxa, 1 continuous and 85 discrete characters was analysed with TNT 1.5. The results show a monophyletic Psalmopoeinae as sister group of Aviculariinae. Psalmopoeus comprises 9 species: Psalmopoeus cambridgei Pocock, 1895 (type species), Psalmopoeus ecclesiasticus Pocock, 1903, Psalmopoeus emeraldus Pocock, 1903, Psalmopoeus irminia Saager, 1994, Psalmopoeus langenbucheri Schmidt, Bullmer & Thierer-Lutz, 2006, Psalmopoeus plantaris Pocock, 1903, Psalmopoeus pulcher Petrunkevitch, 1925, Psalmopoeus reduncus (Karsch, 1880), and Psalmopoeus victori Mendoza, 2014. Psalmopoeus intermedius Chamberlin, 1940 is considered a junior synonym of P. reduncus. Psalmopoeus copanensis Gabriel & Sherwood, 2020, P. sandersoni Gabriel & Sherwood, 2020 and P. petenensis Gabriel & Sherwood, 2020 are considered junior synonyms of P. victori. Psalmopoeus maya Witt, 1996 is considered nomen nudum. Tapinauchenius comprises 8 species: Tapinauchenius plumipes (C. L. Koch, 1842) (type species), Tapinauchenius sanctivincenti (Walckenaer, 1837), Tapinauchenius latipes L. Koch, 1875, Tapinauchenius brunneus Schmidt, 1995, Tapinauchenius cupreus Schmidt & Bauer, 1996, Tapinauchenius polybotes Hüsser, 2018, Tapinauchenius rasti Hüsser, 2018, and T. gretae n. sp. The female of T. brunneus is described for first time. Tapinauchenius violaceus (Mello-Leitão, 1930), T. purpureus Schmidt, 1995, T. concolor (Caporiaccco, 1947), and T. gigas Caporiacco, 1954 are considered junior synonyms of T. plumipes. With the synonymy of T. gigas (type species of Pseudoclamoris Hüsser, 2018), it was necessary to describe a new genus for the two species formerly included in it. Thus, the new genus Amazonius n. gen. is erected to include A. elenae (Schmidt, 1994) n. comb., A. burgessi (Hüsser, 2018) n. comb. as well as two new species A. giovaninii n. gen. n. sp. and A. germani n. gen. n. sp. A discussion on the relationship of Psalmopoeinae and Aviculariinae and maps with the distributions of all species are provided.

Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga.

Theraphosidae spider venoms are well known for possess a complex mixture of protein and non-protein compounds in their venom. The objective of this study was to report and identify different proteins translated from the venom gland DNA information of the recently described Theraphosidae spider Pamphobeteus verdolaga. Using a venom gland transcriptomic analysis, we reported a set of the first complete sequences of seven different proteins of the recenlty described Theraphosidae spider P. verdolaga. Protein analysis indicates the presence of different proteins on the venom composition of this new spider, some of them uncommon in the Theraphosidae family. MS/MS analysis of P. verdolaga showed different fragments matching sphingomyelinases (sicaritoxin), barytoxins, hexatoxins, latroinsectotoxins, and linear (zadotoxins) peptides. Only four of the MS/MS fragments showed 100% sequence similarity with one of the transcribed proteins. Transcriptomic analysis showed the presence of different groups of proteins like phospholipases, hyaluronidases, inhibitory cysteine knots (ICK) peptides among others. The three database of protein domains used in this study (Pfam, SMART and CDD) showed congruency in the search of unique conserved protein domain for only four of the translated proteins. Those proteins matched with EF-hand proteins, cysteine rich secretory proteins, jingzhaotoxins, theraphotoxins and hexatoxins, from different Mygalomorphae spiders belonging to the families Theraphosidae, Barychelidae and Hexathelidae. None of the analyzed sequences showed a complete 100% similarity.

Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga

Theraphosidae spider venoms are well known for possess a complex mixture of protein and non -protein compounds in their venom. The objective of this study was to report and identify different proteins translated from the venom gland DNA information of the recently described Theraphosidae spider Pamphobeteus verdolaga. Using a venom gland transcriptomic analysis, we reported a set of the first complete sequences of seven different proteins of the recenity described Theraphosidae spider P. verdolaga. Protein analysis indicates the presence of different proteins on the venom composition of this new spider, some of them uncommon in the Theraphosidae family. MS/MS analysis of P. verdolaga showed different fragments matching sphingomyelinases (sicaritoxin), barytoxins, hexatoxins, latroinsectotoxins, and linear (zadotoxins) peptides. Only four of the MS/MS fragments showed 100% sequence similarity with one of the transcribed proteins. Transcriptomic analysis showed the presence of different groups of proteins like phospholipases, hyaluronidases, inhibitory cysteine knots (ICK) peptides among others. The three database of protein domains used in this study (Pfam, SMART and CDD) showed congruency in the search of unique conserved protein domain for only four of the translated proteins. Those proteins matched with EF-hand proteins, cysteine rich secretory proteins, jingzhaotoxins, theraphotoxins and hexatoxins, from different Mygalomorphae spiders belonging to the families Theraphosidae, Barychelidae and Hexathelidae. None of the analyzed sequences showed a complete 100% similarity

Aguapanela, a new tarantula genus from the Colombian Andes (Araneae, Theraphosidae).

A new monotypic genus of Theraphosidae is described from Colombia: Aguapanela Perafán & Cifuentes gen. nov. with only the type species Aguapanela arvi Perafán, Cifuentes & Estrada sp. nov., from Caldas and Medellin, Antioquia, Colombian Andes. The new genus differs from other theraphosid spiders mainly in the presence of stridulatory setae on the palps and legs I and II, together with the presence of type III and IV urticating setae. Males lack a tibial apophysis on leg I and have a simple palpal bulb with the subtegulum less extended than usual in Theraphosinae, elongated curved embolus, ventrally concave, and with two prolateral keels very flat and developed on the dorsal edge. The female spermathecae have two digitiform elongated and granulated seminal receptacles attached to a semicircular wide membranous base. We describe, diagnose and illustrate the new genus and give some biological remarks. Morphological, systematic and biogeographic aspects are discussed. Chromatographic and electrophoretic profiles of its venom are analyzed.