Toxicity thresholds for juvenile freshwater mussels Echyridella menziesii and crayfish Paranephrops planifrons, after acute or chronic exposure to Microcystis sp.

Survival of juvenile freshwater mussels (Echyridella menziesii (Gray, 1843) formerly known as Hyridella menziesi) and crayfish (Paranephrops planifrons, White, 1842) decreased after four days exposure to microcystin-containing cell-free extracts (MCFE) of Microcystis sp. at concentrations typical of severe cyanobacterial blooms. Crayfish survival was 100, 80, and 50% in microcystin concentrations of 1339, 2426, and 11146 µg L(-1) respectively, and shade- and shelter-seeking behavior was negatively affected when concentrations were ≥2426 µg L(-1) . Mussel survival decreased to 92% and reburial rates decreased to 16% after exposure for 96 h to MCFE containing microcystins at concentrations of 5300 µg L(-1) . Crayfish survival was 100% when fed freeze-dried Microcystis sp. incorporated into an artificial diet (6-100 µg microcystin kg(-1) ww) at dietary doses from 0.03 to 0.55 µg g(-1) body weight d(-1) for 27 days. Specific growth rate was significantly lower in crayfish fed ≥0.15 µg g(-1) body weight day(-1) compared with controls, but not compared with a diet incorporating nontoxic cyanobacteria. Microcystins accumulated preferentially in crayfish hepatopancreas and mussel digesta as MCFE or dietary concentrations increased. These laboratory data indicate that, assuming dissolved oxygen concentrations remain adequate, and no simultaneous exposure to live Microcystis sp. cells, cell-free microcystins will only be a significant stressor to juvenile crayfish and mussels in severe Microcystis sp. blooms. In contrast, crayfish were negatively affected by relatively low concentrations of microcystins in artificial diets compared with those measured locally in benthic cyanobacterial mats.

Controlling the invasive diatom Didymosphenia geminata: an ecotoxicity assessment of four potential biocides.

In 2004, an invasive mat-forming freshwater diatom, Didymosphenia geminata (didymo), was found in New Zealand causing concern with regard to potential consequences for local freshwater ecosystems. A four-stage research program was initiated to identify methods to control D. geminata. This article reports the results of Stage 2, in which four potential control compounds [Gemex™ (a chelated copper formulation), EDTA, Hydrothol®191, and Organic Interceptor™ (a pine oil formulation)] selected in Stage 1 were evaluated for their biocidal efficacy on D. geminata and effects on non-target organisms using both artificial stream and laboratory trials. Artificial stream trials evaluated the mortality rates of D. geminata and fishes to three concentrations of the four biocides, whereas laboratory toxicity trials tested the response of green alga and cladocera to a range of biocide concentrations and exposure times. In artificial stream trials, Gemex and Organic Interceptor were the most effective biocides against D. geminata for a number of measured indices; however, exposure of fishes to Organic Interceptor resulted in high mortality rates. Laboratory toxicity testing indicated that Gemex might negatively affect sensitive stream invertebrates, based on the cladoceran sensitivity at the proposed river control dose. A decision support matrix evaluated the four biocides based on nine criteria stipulated by river stakeholders (effectiveness, non-target species impacts, stalk removal, degradation profile, risks to health and safety, ease of application, neutralization potential, cost, and local regulatory requirements) and Gemex was identified as the product warranting further refinement prior to an in-river trial.

Control of chironomid midge larvae in wastewater stabilisation ponds: comparison of five compounds.

Chironomid midge larvae are a valuable component of wastewater stabilisation pond (WSP) ecology. However, in high numbers, adult midge swarms can be a nuisance to near-by urban areas. Improving WSP treatment by incorporating aerobic or maturation ponds or by the addition of pre-treatment to reduce organic loading also increases the availability of aerobic sediment (midge larva habitat) in the pond system and the potential for midge nuisance problems. The efficacy of Maldison, an organophosphate traditionally used to control midge larvae in New Zealand WSPs, was compared to Bacillus thuringiensis var. israelensis (Bti), Methoprene, Pyriproxyfen and Diflubenzuron which are all more specific to insects and have fewer adverse environmental effects. Initial laboratory trials established the concentration of each compound required to achieve 95% control of the midge population. During 21-day small-scale trials within the WSP, Bti, Diflubenzuron and Maldison reduced live larvae numbers substantially (80-89%) compared to controls and adult midge emergence was markedly reduced by all compounds (72-96%). Large-scale trials with Bti (Vectobac WG) powder (1000 microg/L) only caused a slight reduction in midge larvae numbers compared to controls and had little effect on adult emergence, however, Methoprene (Prolink XRG granules) (50 microgAI/L) reduced midge adult emergence by approximately 80% over 25 days and has been used successfully to control several midge nuisance outbreaks.

Gastrointestinal uptake and distribution of copper in rainbow trout.

A single dose of radioactive copper ((64)Cu or new Cu) was infused into the stomach of rainbow trout (Oncorhynchus mykiss) to model dietary copper (Cu) uptake under conditions of a normal nutritional dose and optimum environmental temperature (16 degrees C, 0.117 microg Cu g(-)(1 )body mass). The distribution of new Cu to the gut and internal organs occurred in two phases: rapid uptake by the gut tissues (almost complete by 24 h post-infusion) followed by slower uptake by the internal organs. By 72 h, 60 % of the dose had been excreted, 19 % was still retained in the gut tissue, 10 % remained in the lumen and 12 % had been absorbed across the gut and partitioned amongst the internal organs. A reduction in water temperature of 10 degrees C (to 6 degrees C) significantly retarded components of new Cu distribution (movement of the bolus along the gut and excretion); nonetheless, by 72 h, the fraction absorbed by all the internal organs was similar to that at 16 degrees C. An increase in water temperature of 3 degrees C (to 19 degrees C) caused a pronounced increase in internal organ uptake by 24 h to approximately double the uptake occurring at 16 degrees C. The uptake of new Cu by the gut tissue had a low temperature coefficient (Q(10)<1) consistent with simple diffusion, while the temperature coefficient for transfer of new Cu from gut tissue to the internal organs was high (Q(10)>2), consistent with facilitated transport. Internally, the liver and gall bladder (including bile) were the target organs for dietary Cu partitioning since they were the only organs that concentrated new Cu from the plasma. Individual tissues differed in terms of the exchange of their background Cu pools with new Cu. The background Cu in the walls of the gastrointestinal tract (excluding stomach) exchanged 45-94 % with new Cu from the gut lumen, while tissues such as the stomach, gills, kidney, carcass and fat had 5-7 % exchangeable background Cu. The liver, heart, spleen, ovary, bile and plasma had only 0.2-0.8 % exchangeable background Cu. The gastrointestinal tissues appear to act as a homeostatic organ, regulating the absorption of nutritional (non-toxic) doses of Cu (0. 117 microg g(-)(1 )body mass day(-)(1)) by the internal organs. Within the dose range we used and at optimal temperature (16 degrees C), the new Cu content of the gut tissues fluctuated, but absorption of new Cu by the internal organs remained relatively constant. For example, predosing the fish with non-radioactive Cu caused new Cu absorption by the gut tissues to double and decreased new Cu excretion from 38 to 1.5 %, but had no effect on new Cu uptake by the internal organs. Feeding fish after application of the normal liquid dose of new Cu also had no effect on new Cu uptake by the internal organs, even though the presence of food in the digestive tract reduced the binding of new Cu to the gut tissues and assisted with the excretion of new Cu. The gut was therefore able to regulate new Cu internalization at this dosage. Higher new Cu doses (10, 100 and 1000 times the normal dose), however, evoked regurgitation and increased new Cu excretion within 4 h of application but did not elevate new Cu levels in gut tissue beyond a threshold of approximately 40 microg of new Cu. Only at the highest dose (1000 times the normal dose, 192 microg g(-)(1 )body mass), equivalent to toxic concentrations in the daily diet (7000 microg Cu g(-)(1 )dry mass food), was the buffering capacity of the gut overwhelmed, resulting in an increase in internal new Cu uptake.

A model of morphogenesis for protein secondary structures.

This paper proposes a model for the expected probability distribution for a certain class of biological structures. In particular, a model is derived for the distribution of lengths of helices, sheets, turns, and coils as a function of the length of the structure divided by the length of the protein it is contained in. A fit between the derived lognormal function and the structures for some proteins whose three-dimensional structure is known was significant. The fit produces fundamental parameters particular to each structure type that are related to the underlying structure and its morphogenesis. The importance of the result is that a universal mathematical distribution can be used to explain certain protein morphogeneses. Also, these fundamental parameters can be used as an aid in predicting whether a given sequence is a particular secondary structure or not, without a knowledge of its three-dimensional structure.

Cooperative solution of constraint satisfaction problems.

It is widely believed that a group of cooperating agents engaged in problem solving can solve a task faster than either a single agent or the same group of agents working in isolation from each other. Nevertheless, little is known about the quantitative improvements that result from cooperation. A number of experimental results are presented on constraint satisfaction that both test the predictions of a theory of cooperative problem solving and assess the value of cooperation for this class of problems. These experiments suggest an alternative methodology to existing techniques for solving constraint satisfaction problems in computer science and distributed artificial intelligence.