RESUMO
The Si capping of Ge/Si(001) islands was observed by in situ time-resolved transmission electron microscopy. During the initial stages of the Si deposition, islands were observed not only to flatten but also to shrink in volume. This unexpected shrinkage is explained by taking into account the intermixing of the deposited Si with the wetting layer and a consequently induced diffusion of Ge from the islands into the wetting layer. A model of the capping process which takes into account Ge diffusion is presented which is in good agreement with the experimental data.
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Electron energy loss spectra are simulated for a C70 crystalline structure. It is found that the simulated spectrum is similar to the unoccupied density of states of a C70 molecule, indicating that the crystalline structure has only a small effect on the spectrum. Unlike the case of a single molecule, however, the main contribution to the second peak in the spectrum cannot be ascribed as being due to the equatorial atoms.
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Zn nanowires with an epitaxial thin surface layer of zinc oxide were dispersed onto amorphous carbon films and stored at room temperature. After 1500 h, a self-organized equal-spaced zinc oxide (approximately 2 nm)/carbon (approximately 2.5 nm) multilayer structure was found to form outside the Zn nanowire, taking the place of the original ZnO surface layer. We carried a systematic study to clarify the self-formation mechanism of the periodical multilayers outside the Zn nanowire and found out that such a configuration originated from a chemical reaction between Zn and CO2 and were formed via a gas phase diffusion-interfacial chemical reaction-phase separation process.
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Coherent Ge(Si)/Si(001) quantum dot islands grown by solid source molecular beam epitaxy at a growth temperature of 700 degrees C were investigated using transmission electron microscopy working at 300kV. The [001] zone-axis bright-field diffraction contrast images of the islands show strong periodicity with the change of the TEM sample substrate thickness and the period is equal to the effective extinction distance of the transmitted beam. Simulated images based on finite element models of the displacement field and using multi-beam dynamical diffraction theory show a high degree of agreement. Studies for a range of electron energies show the power of the technique for investigating composition segregation in quantum dot islands.
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Using a conventional transmission electron microscope that incorporates a field emission gun it is possible to focus an electron beam to form a small probe (<1nm full-width at half-maximum). Such a probe can then be used to perform high spatial resolution diffraction experiments. The high spatial resolution allows technologically interesting amorphous volumes, such as those found in glassy intergranular phases or in semiconductor implantations, to be investigated directly. In order to achieve the probe characteristics necessary to investigate nanovolumes of material the probe must be highly convergent which results in it being highly coherent. In this paper we examine the effect of coherent convergent illumination on electron diffraction data taken from nanovolumes of amorphous material. It is shown that, for amorphous volumes as small as 1.2nm in diameter, the additional interference effects induced in the diffraction data by the use of coherent convergent illumination are largely suppressed by the lack of order in amorphous materials. This allows the use of deconvolution techniques, developed for the correction of broadening of the diffraction pattern in the case of incoherent illumination, and the subsequent application of reduced density function (G(r)) analysis, to also be used for coherent illumination.
RESUMO
To perform reduced density function (G(r)) analysis on electron diffraction patterns of amorphous materials formed with convergent beams, the effects of convergence must be removed from the diffraction data. Assuming electrons incident upon the sample in different directions are incoherent, this can be done using deconvolution (Ultramicroscopy 76 (1999) 115). In this letter we show that the combination of an energy filtering transmission electron microscope with an image plate, increases the accuracy with which diffraction data can be measured and, subsequently, the accuracy of the deconvolution.
Assuntos
Microscopia Eletrônica/métodos , Algoritmos , Carbono , Cristalografia/métodos , Elétrons , Microscopia Eletrônica/instrumentação , Análise EspectralRESUMO
The s-state approach is useful for analysing transmission electron microscope images of a thin crystalline foil consisting of well-separated atomic columns. It assumes that the signal collected (e.g., the annular dark-field image, the EELS spectrum) can be attributed to only the lowest-energy bound eigenstate of the two-dimensional projected potential of a single column. When, however, columns are close, the form of the bound states depends on more than one column, which implies that interpretation of the signal may not be so simple. For closely spaced columns we show that the simple s-state approach fails for the case of a sub-Angstrom probe initially centred on one column of a pair, because two bound states are excited. The energy in the probe is almost completely transferred to the neighbouring column after it has propagated some tens of nanometres through the foil and then is transferred back. Signals which relate directly to the local probe intensity (e.g. annular dark-field formed by thermal diffuse scattering, EELS) must be analysed in terms of the two bound states. Accurate calculations of bound states of pairs of columns are more demanding than for a single column but sufficient accuracy can be achieved from knowledge of the 1s-states of isolated columns. We provide formulae for the bound states of a column pair. These can be used to determine if image analysis requires the extension to the s-state approach described in this paper.
Assuntos
Algoritmos , Microscopia Eletrônica de Transmissão e Varredura/métodos , Arsênio , Microanálise por Sonda Eletrônica/métodos , Análise de Fourier , Gálio , Espalhamento de Radiação , Análise EspectralRESUMO
BACKGROUND & AIMS: Cystic fibrosis transmembrane conductance regulator (CFTR) is an adenosine 3',5'-cyclic monophosphate-dependent chloride channel that is defective in cystic fibrosis. The aims of this study were to determine if defective apical chloride secretion in the intestine of patients with cystic fibrosis alters the intracellular electrolyte milieu and to examine the geographical localization of CFTR in the normal intestine. METHODS: The content of intracellular elements was assessed in cryosections using energy-dispersive x-ray microanalysis, and CFTR was identified by immunocytochemistry using commercially available antibodies. RESULTS: Cystic fibrosis jejunum had a significantly lower Na+ content, higher K+ and Cl- content, and higher potassium/phosphorus ratio in both villus and crypt regions. Incubation of normal jejunum with the phosphodiesterase inhibitor 3-isobutyl-1-methyl-xanthine (300 mumol/L) resulted in decreased K+ and Cl- content in both crypt and villus regions, indicative of Cl- secretion. CFTR was identified on the surface of normal villus and crypt enterocytes but not in cystic fibrosis samples. CONCLUSIONS: Defective apical chloride channels in cystic fibrosis result in alterations in the intracellular electrolyte milieu. The microanalysis observations and immunocytochemical studies imply a role for villus enterocytes in human intestinal chloride secretion.
Assuntos
Cloretos/metabolismo , Fibrose Cística/metabolismo , Mucosa Intestinal/metabolismo , Jejuno/metabolismo , 1-Metil-3-Isobutilxantina/farmacologia , Animais , Regulador de Condutância Transmembrana em Fibrose Cística/metabolismo , Microanálise por Sonda Eletrônica , Humanos , Imuno-Histoquímica , Mucosa Intestinal/efeitos dos fármacos , Jejuno/efeitos dos fármacos , Inibidores de Fosfodiesterase/farmacologia , Fósforo/metabolismo , Potássio/metabolismo , Coelhos , Sódio/metabolismoRESUMO
1. Proximal tubular intracellular elements were measured by electron microprobe X-ray analysis (a) in rats volume-expanded with albumin-saline in which peritubular oncotic pressure remained normal and (b) in rats in which the renal artery was snared before volume expansion (the early snare model). Glomerular filtration rate and urine Na+ excretion were measured in addition to intracellular Rb+ following a 30 s infusion of RbCl as a marker for K+ transport. 2. In albumin-saline volume-expanded rats, intracellular levels of Na+ ([Na+]i) at 21.5 +/- 0.6 mmol (kg wet wt)-1, Cl- ([Cl-]i) at 18.0 +/- 0.4 mmol (kg wet wt)-1 and Rb+ ([Rb+]i) at 9.4 +/- 0.4 mmol (kg wet wt)-1 were significantly higher (P < 0.0001) than the levels in non-expanded rats ([Na+]i, [Cl-]i and [Rb+]i at 17.7 +/- 0.4, 14.6 +/- 0.3 and 4.7 +/- 0.4 mmol (kg wet wt)-1, respectively; means +/- S.E.M.). The data are consistent with Na+ pump inhibition in the proximal tubule, although this cannot be directly derived from intracellular element measurements. 3. In an early snare model of volume expansion, [Na+]i, intracellular K+ ([K+]i) and [Rb+]i remained unchanged (16.1 +/- 0.4, 131.0 +/- 2.0 and 5.2 +/- 0.3 mmol (kg wet wt)-1, respectively) compared to non-expanded snared kidneys (15.9 +/- 0.6, 131.3 +/- 1.8 and 4.8 +/- 0.3 mmol (kg wet wt)-1, respectively). [Cl-]i at 18.3 +/- 0.5 mmol (kg wet wt)-1 increased (P < 0.0008) compared to controls at 15.8 +/- 0.5 mmol (kg wet wt)-1. Thus, in these rats, evidence for an inhibition of the Na+ pump was no longer observed. This points to a major intrinsic mechanism within the kidney for mediating natriuresis, since circulating factors were identical to those in the unsnared kidney, where significant natriuresis occurred.
Assuntos
Eletrólitos/metabolismo , Túbulos Renais Proximais/fisiologia , Sódio/metabolismo , Animais , Natriurese/fisiologia , Perfusão , Potássio/metabolismo , Pressão , Ratos , Rubídio/metabolismoRESUMO
1. In order to further define the action of atrial natriuretic peptide (ANP) on proximal tubular (PT) transport, combined clearance and electron microprobe X-ray (EMPX) experiments were performed on five male Wistar rats infused with ANP (0.16 nmol/kg per h) and nine control animals. 2. Electron microprobe X-ray analysis of PT cell electrolytes (mmol/kg wet weight) revealed a similar [Na]i in both the control and ANP treated groups (16.4 +/- 0.4 vs 16.5 +/- 0.4; P = 0.894). [Cl]i was lower in the ANP treated animals (14.8 +/- 0.3 vs 12.0 +/- 0.3; P < 0.0001) as was [K]i (131.4 +/- 1.4 vs 114 +/- 1.7; P < 0.0001). The PT cells in the ANP treated group had a significant reduction in dry weight (20.1 +/- 0.3 g% vs 19.0 +/- 0.3 g%; P < 0.024), indicating significant cell swelling. Thus, despite a normal [Na]i, there was net accumulation of Nai following ANP treatment. 3. These results are consistent with accumulation of Nai due to inhibition of the Na pump followed by cell swelling and subsequent regulatory volume decrease with exit of K and Cl. These results are the first to show the effect of ANP on PT intracellular electrolytes.
Assuntos
Fator Natriurético Atrial/farmacologia , Túbulos Renais Proximais/efeitos dos fármacos , Túbulos Renais Proximais/metabolismo , ATPase Trocadora de Sódio-Potássio/antagonistas & inibidores , Animais , Eletrólitos/metabolismo , Microanálise por Sonda Eletrônica , Taxa de Filtração Glomerular/efeitos dos fármacos , Masculino , Concentração Osmolar , Potássio/metabolismo , Ratos , Ratos Wistar , Trocadores de Sódio-Hidrogênio/antagonistas & inibidoresRESUMO
The interpretation of element concentration data for X-ray microanalyses of biological tissues, which are subjected to some experimental treatment, can be complicated by changes in cell volume and total cell dry matter induced by the treatment. We have examined the manner in which such changes would affect the values measured in frozen-dried cryosections of soft tissues, and how they may be taken into account in the interpretation of the results. The element content (mass per unit dry weight) measured by the peak-to-continuum or Hall method is independent of changes in cell volume, but is sensitive to a change in the local dry mass. Conversely, intracellular concentrations in terms of mass per unit volume, as determined by the peripheral or internal standard technique, are dependent on volume changes but independent of dry mass. The estimated dry weight fraction is affected by changes in both volume and dry mass. The results obtained from both quantification methods can therefore provide information on the combination of changes in cellular element levels, volume and total dry mass that may occur following the experimental treatment. In a study of the late effect of the drug cisplatin on electrolyte concentrations in kidney proximal tubules, both quantification methods have been used to obtain wet weight and dry weight concentrations. By applying the above considerations, the analytical results have been interpreted as a combination of changes in element levels and a shrinkage of the tubule cells. Cell shrinkage was confirmed by morphometric analysis of tubular cross-sections.
Assuntos
Cisplatino/farmacologia , Eletrólitos/análise , Microanálise por Sonda Eletrônica , Túbulos Renais Proximais/efeitos dos fármacos , Animais , Secções Congeladas , Túbulos Renais Proximais/química , Túbulos Renais Proximais/citologia , Masculino , Ratos , Ratos EndogâmicosRESUMO
Electron microprobe X-ray analysis techniques were employed in order to assess the changes that occur in proximal tubular cell sodium concentration during the hyperfiltration phase of early diabetes mellitus induced by streptozotocin in Sprague Dawley rats. Intracellular rubidium accumulation following intravenous infusion of rubidium chloride was used as a marker of basolateral Na/K-ATPase activity. The diabetic animals studied had a significantly higher glomerular filtration rate compared with controls [1.44 +/- 0.07 vs. 1.00 +/- 0.07 ml min-1 (100 g body weight)-1; mean +/- SEM, P less than 0.001]. Intracellular Na concentration was significantly higher in diabetic animals (19.5 +/- 0.6 vs. 17.8 +/- 0.4 mmol/kg wet weight; P less than 0.01). Concurrent measurement of Rb demonstrated significantly higher intracellular accumulation in the proximal tubules of diabetic animals compared with control (7.9 +/- 0.5 vs. 5.5 +/- 0.5 mmol/kg wet weight; P less than 0.001). These results indicate that proximal tubular Na/K-ATPase activity is enhanced in the hyperfiltration phase of diabetes mellitus. Since, however, intracellular Na concentration is increased under these conditions, it may be inferred that apical Na entry into proximal tubular cells is stimulated beyond the rate of basal exit during the initial development of hyperfiltration. The reasons for these alterations in cellular Na transport are unclear but similar changes have been implicated in the pathogenesis of cell growth.
Assuntos
Nefropatias Diabéticas/metabolismo , Túbulos Renais Proximais/metabolismo , Sódio/metabolismo , Animais , Diabetes Mellitus Experimental/metabolismo , Microanálise por Sonda Eletrônica , Masculino , Ratos , Ratos Endogâmicos , ATPase Trocadora de Sódio-Potássio/metabolismoRESUMO
The earliest phase of cisplatin nephrotoxicity involves natriuresis due to impaired sodium reabsorption by the proximal tubule. To define the cell mechanism of this transport lesion, electron microprobe X-ray analysis was used to determine changes in the electrolyte composition of proximal tubule cells in kidneys taken from rats treated acutely with cisplatin (1 mg/100 g body weight). Compared to control animals injected with vehicle, cisplatin treated rats developed significant natriuresis, the fractional excretion of sodium rising over sevenfold. In kidneys removed 90 min following cisplatin, sodium concentration in proximal tubule cells was reduced by 4.2 mmol/kg wet weight, or 19%, compared to control values. When allowance was made for cell shrinkage in cisplatin-treated kidneys by deriving the cell content of sodium (mmol/kg dry weight), the reduction was even greater (28%). These data suggest that cisplatin reduces proximal tubule sodium reabsorption by inhibiting the entry of sodium into the cells across the apical membrane.
Assuntos
Cisplatino/toxicidade , Túbulos Renais Proximais/efeitos dos fármacos , Sódio/metabolismo , Animais , Transporte Biológico/efeitos dos fármacos , Microanálise por Sonda Eletrônica , Túbulos Renais Proximais/metabolismo , Masculino , Ratos , Ratos EndogâmicosRESUMO
We have prepared and studied seventeen thin films ranging in composition from pure silver to pure magnesium fluoride. Using mass thicknesses based on quartz crystal oscillators and physical thicknesses obtained from a stylus profilometer, we have measured the void volume fraction of the films as a function of their composition. We have shown the Ag-MgF(2) system to be capable of incorporating a high volume fraction of voids. We report on the results of electron microscope studies of the films, which show the dependence of film microstructure on composition, thickness, and the nature of its substrate.
RESUMO
In the preparation of microdroplets of biological fluids for X-ray microanalysis, we have found that incorporation of a macromolecular solute, dextran, to a final concentration of 1.5-2.5% retards crystal formation and produces sufficiently uniform deposits on thin films to allow droplets to be analysed without prior freeze-drying. Analyses have been carried out at 20 kV in a scanning electron microscope, using energy-dispersive spectrometry. Absorption of Na X-rays by the added solute can be significant but its effect is minimized by preparing droplets as thin as possible, and by using standards of similar composition. The minimum detectable concentrations are increased because of the extra background contribution, and for a single determination are about 6 mM for Na and 2 mM for Cl and K. These concentrations can be further reduced by measuring replicates. The reproducibility of analysis is significantly improved (to less than 5% for Na and K) over the use of calibration curves by calculating the element concentrations from a known element in the sample, chlorine. Under our analytical conditions loss of Cl did not occur. This method requires that the Cl is measured separately by microcoulometry, but eliminates the need for a range of standard droplets on the grid, and determination of the unknowns is then independent of droplet volume, beam current, counting time and magnification. We have compared, with biological samples, the results from using Cl as an internal standard with those obtained using an added standard element, cobalt. The reproducibility using Cl was approximately two-times better than that obtained with Co, probably because of unavoidable volumetric errors when the Co is pipetted separately.
Assuntos
Cloro/normas , Dextranos , Microanálise por Sonda Eletrônica , Urina/análise , Cálcio/análise , Cobalto/normas , Humanos , Magnésio/análise , Microscopia Eletrônica de Varredura , Fósforo/análise , Potássio/análise , Padrões de Referência , Sódio/análiseRESUMO
The optical properties of MgF(2) films prepared by evaporation and ion-assisted deposition have been determined from transmittance and near-normal incidence reflectance measurements and also from electron-energy loss spectroscopy (EELS). The results show that oxygen-ion assistance leads to higher extinction coefficients for wavelengths <180 nm. Transmission electron microscopy studies show that the crystal grain size of MgF(2) films is not strongly affected by oxygen or argon-ion bombardment. The presence of MgO in the films is inferred from RBS measurements and proposed to be the major factor influencing VUV losses. EELS is also demonstrated to be a valuable technique for determination of optical properties from the near-infrared to x-ray regions of the spectrum.
RESUMO
Centrifuged pellets of turbid parotid saliva from cystic fibrosis (CF) patients and non-CF subjects, obtained from saliva kept at 2 degrees for 10 min, had the electron microscope appearance of amorphous, round particles, and were thought to be colloidal aggregates of organic material. Drops of turbid saliva, from samples incubated for 2 hr at 2 degrees or 37 degrees, additionally contained discrete, electron-dense crystals having well defined angular morphology: usually cubic, retangular, or approximately hexagonal. The inhibitors, urea, guanidine hydrochloride, and EDTA, resulted in no crystals being observed. Selected area electron diffraction from individual crystals showed predominantly hexagonal, rectangular patterns could be indexed as coming from hydroxyapitite. A transition from the hexagonal to the rectangular pattern and back to the hexagonal pattern could be obtained from individual crystals tilted in the electron microscope. The square diffraction pattern may be from octa-calcium or brushite. Polyacrylamide gel disc electrophoresis of the parotid saliva indicated that the sparingly soluble proteins in the 2 degree and 37 degree pellets comprised proline-rich proteins and a calcium-precipitable, trichloroacetic acid (TCA)-precipitable phosphoprotein, which fluoresced with amido schwarz and Coomassie brilliant blue G250.