Electrochemical characteristics of ultramicro-dimensioned SIROF electrodes for neural stimulation and recording.

OBJECTIVE: With ever increasing applications of neural recording and stimulation, the necessity for developing neural interfaces with higher selectivity and lower invasiveness is inevitable. Reducing the electrode size is one approach to achieving such goals. In this study, we investigated the effect of electrode geometric surface area (GSA), from 20 µm2 to 1960 µm2, on the electrochemical impedance and charge-injection properties of sputtered iridium oxide (SIROF) coated electrodes in response to current-pulsing typical of neural stimulation. These data were used to assess the electrochemical properties of ultra-small SIROF electrodes (GSA < 200 µm2) for stimulation and recording applications. APPROACH: SIROF charge storage capacities (CSC), impedance, and charge-injection characteristics during current-pulsing of planar, circular electrodes were evaluated in an inorganic model of interstitial fluid (model-ISF). MAIN RESULTS: SIROF electrodes as small as 20 µm2 could provide 1.3 nC/phase (200 µs pulse width, 0.6 V versus Ag|AgCl interpulse bias) of charge during current pulsing. The 1 kHz impedance of all electrodes used in this study were below 1 MΩ, which is suitable for neural recording. SIGNIFICANCE: Ultra-small SIROF electrodes are capable of charge injection in buffered saline at levels above some reported thresholds for neural stimulation with microelectrodes.

Photodiode circuits for retinal prostheses.

Photodiode circuits show promise for the development of high-resolution retinal prostheses. While several of these systems have been constructed and some even implanted in humans, existing descriptions of the complex optoelectronic interaction between light, photodiode, and the electrode/electrolyte load are limited. This study examines this interaction in depth with theoretical calculations and experimental measurements. Actively biased photoconductive and passive photovoltaic circuits are investigated, with the photovoltaic circuits consisting of one or more diodes connected in series, and the photoconductive circuits consisting of a single diode in series with a pulsed bias voltage. Circuit behavior and charge injection levels were markedly different for platinum and sputtered iridium-oxide film (SIROF) electrodes. Photovoltaic circuits were able to deliver 0.038 mC/cm(2) (0.75 nC/phase) per photodiode with 50- µm platinum electrodes, and 0.54-mC/cm(2) (11 nC/phase) per photodiode with 50-µ m SIROF electrodes driven with 0.5-ms pulses of light at 25 Hz. The same pulses applied to photoconductive circuits with the same electrodes were able to deliver charge injections as high as 0.38 and 7.6 mC/cm(2) (7.5 and 150 nC/phase), respectively. We demonstrate photovoltaic stimulation of rabbit retina in-vitro, with 0.5-ms pulses of 905-nm light using peak irradiance of 1 mW/mm(2). Based on the experimental data, we derive electrochemical and optical safety limits for pixel density and charge injection in various circuits. While photoconductive circuits offer smaller pixels, photovoltaic systems do not require an external bias voltage. Both classes of circuits show promise for the development of high-resolution optoelectronic retinal prostheses.

Assessing polarization of AIROF microelectrodes.

Activated Iridium Oxide Film (AIROF) microelectrodes are being proposed for use in multiple neural prosthesis designs because they are characterized by a high charge-delivery capacity. Implicit in their use, is the restriction of limiting the electrode polarization within limits that do not initiate water electrolysis at the electrode/electrolyte interface. These limits, the so-called "water window," are used to ensure that the AIROF electrodes can deliver charge reversibly in various electrolyte environments. Here, we present data from a set of experiments designed to refine the polarization limit criteria used for AIROF electrodes, in vivo. We observe the presence of a secondary ohmic voltage drop that is not seen in vitro. We hypothesize that this secondary ohmic drop may be caused by ion depletion within the AIROF pore structure. The magnitude of this ohmic drop appears to be a function of film thickness, increasing for thicker films. Although increasing the thickness of the AIROF can significantly increase its charge delivery capacity in vitro, the consequence of the thicker film, with respect to deliverable charge, is minimal and can be even detrimental for the in vivo environment. We believe that this phenomenon is mainly due to the ionic inaccessibility of the porous layer structure of the iridium oxide. This study may have widespread consequences for numerous neural prosthesis designs presently being developed, worldwide.

In vitro and in vivo charge capacity of AIROF microelectrodes.

Activated Iridium Oxide Film (AIROF) microelectrodes are thought to be well-suited for neural stimulation of the cortex because they can sustain high charge capacity (about ten times higher than Pt microelectrodes) when characterized in phosphate-buffered saline (PBS) or other high ionic strength electrolytes. However, it is known that their capacity diminishes after they are implanted in vivo. It has been suggested that tissue encapsulation is an underlying cause. In this paper, we report electrochemical measurements of AIROF microelectrodes that were performed acutely in the brain of the zebra finch. The experiment showed that the interstitial fluid environment in the bird's brain did not maintain the high charge delivery capacity of the AIROF microelectrodes. A simple compensation for access resistance may create hazards to sustained electrode integrity.

"Safe" charge-injection waveforms for iridium oxide (AIROF) microelectrodes.

Use of anodic bias improves the charge-injection limits of activated iridium oxide (AIROF) microelectrodes. Asymmetric waveforms, in which the charge balancing anodic phase is delivered at a lower current density and longer pulse width, has been found to allow for higher values of anodic bias voltages, thus maximizing the AIROF charge-injection capacity. Limiting the voltage excursion of the AIROF below the value at which electrolysis of water occurs is essential to maintaining the long-term viability of implanted electrodes. However, maintaining the electrodes at an anodic bias state while keeping the electrode voltage within these electrochemically "safe" limits complicates the topology of the electronic driver circuitry. We present two possible driver topologies that use compliance-voltage limitation in combination with cathodic current modification.

Sputtered iridium oxide films (SIROFs) for low-impedance neural stimulation and recording electrodes.

Iridium oxide films formed by electrochemical activation of iridium metal (AIROF) or by electrochemical deposition (EIROF) are being evaluated as low-impedance charge-injection coatings for neural stimulation and recording. Iridium oxide may also be deposited by reactive sputtering from iridium metal in an oxidizing plasma. The characterization of sputtered iridium oxide films (SIROFs) as coatings for nerve electrodes is reported. SIROFs were characterized by cyclic voltammetry, electrochemical impedance spectroscopy, and potential transient measurements during charge-injection. The surface morphology of the SIROF transitions from smooth to highly nodular with increasing film thickness from 80 nm to 4600 nm. Charge-injection capacities exceed 0.75 mC/cm(2) with 0.75 ms current pulses in thicker films. The SIROF was deposited on both planar and non-planar substrates and photolithographically patterned by lift-off.

A laboratory testing and driving system for AIROF microelectrodes.

The charge-injection currents of AIROF (activated iridium oxide film) microelectrodes, which are subjected to charge-balanced biphasic pulsing or monophasic current pulsing, have to be limited such that the anodic and cathodic voltage excursions are kept within safe limits of operation. In earlier studies it has been shown that when using anodic bias asymmetry in the magnitude of the balanced biphasic waveform can be used to increase the charge injection capacity of AIROF electrodes. We present the design of a single-channel testing and driving system for laboratory testing and driving of AIROF microelectrodes within safe charge-injection limits.

Electrodeposited iridium oxide for neural stimulation and recording electrodes.

Iridium oxide films formed by electrodeposition onto noniridium metal substrates are compared with activated iridium oxide films (AIROFs) as a low impedance, high charge capacity coating for neural stimulation and recording electrodes. The electrodeposited iridium oxide films (EIROFs) were deposited on Au, Pt, PtIr, and 316 LVM stainless steel substrates from a solution of IrCl4, oxalic acid, and K2CO3. A deposition protocol involving 50 potential sweeps at 50 mV/s between limits of 0.0 V and 0.55 V (versus Ag AgCl) followed by potential pulsing between the same limits produced adherent films with a charge storage capacity of >25 mC/cm2. Characterization by cyclic voltammetry and impedance spectroscopy revealed no differences in the electrochemical behavior of EIROF on non-Ir substrates and AIROF. The mechanical stability of the oxides was evaluated by ultrasonication in distilled water followed by dehydration and rehydration. Stability under charge injection was evaluated using 200 micros, 5.9 A/cm2 (1.2 mC/cm2) cathodal pulses. Loss of iridium oxide charge capacity was comparable for AIROFs and the EIROFs, ranging from 1% to 8% of the capacity immediately after activation or deposition. The EIROFs were deposited and evaluated on silicon microprobe electrodes and on metallized polyimide electrodes being developed for neural recording and stimulation applications.

Comparison of 316LVM and MP35N alloys as charge injection electrodes.

An In vitro comparison of the corrosion response of 316LVM stainless steel and MP35N (a CoNiCrMo alloy) electrodes under conditions appropriate to applications in functional electrical stimulation (FES) was made. Electrodes of both alloys were subjected to a cathodic 40 microC/cm2 charge injection protocol and the potential transient response was recorded over a 96 h period. The transient responses were compared with potentiodynamic polarization data used to establish the quasiequilibrium response of the alloys in the carbonate and phosphate-buffered saline electrolyte used in the study. The MP35N electrodes exhibited extensive pitting corrosion during charge injection, whereas little corrosion was observed on 316LVM electrodes. An explanation for the susceptibility of MP35N to corrosion during charge injection is found in the potentiodynamic polarization data, which reveal a breakdown potential (critical pitting potential) of 0.45 V (SCE) for MP35N compared with 1.05 V (SCE) for 316LVM. Factors that may influence corrosion response during charge injection from alloys exhibiting active-passive behavior are discussed.

Evaluation of direct bladder stimulation with stainless steel woven eye electrodes.

Encouraged by recent clinical reports of micturition induced in patients by direct bladder stimulation, we conducted a study of optimum methods of direct bladder stimulation. During surgery six male cats received eight large surface-area woven eye electrodes sutured to the bladder wall serosa, four on the bladder dome and four adjacent to the trigone area. Two additional small surface-area single knot electrodes were sutured in the trigone area. Suprapubic and intraperitoneal tubes were placed for pressure recording and bladder filling. Leg and pelvic floor EMG electrodes were also used for tethered recordings. One to eight weeks after surgery, optimum stimulation methods were evaluated as the animal freely moved about a urodynamic recording cage. Electrodes in the trigone region were more effective than electrodes on the dome and induced bladder contractions and voiding similar to spontaneously induced voiding with bladder filing. Large surface area, woven eye electrodes, composed of multistranded 316LVM stainless steel wire, were more effective than smaller surface area single knot electrodes. High stimulating frequencies (40 Hz) were better than lower frequencies (10 to 20 Hz), and a 1 millisecond pulse duration was optimal. Pulsing with stimulating currents from 10 to 25 mA induced effective bladder contractions with voiding when applied for 3 seconds. However, lower currents using longer stimulation periods were also effective. Bipolar electrodes with both electrodes on the bladder wall were superior to monopolar arrangements with the positive ground electrode along the animal's back. We concluded that in the able-bodied cat model, bladder contractile activity for micturition can be induced with direct bladder stimulation and with little discomfort. An effective stimulation protocol consists of capacitor-coupled monophasic pulses with large surface area bipolar electrodes in the trigone region. Stimulating parameters of 40 Hz, 1 msec., 10 to 25 mA applied for 3 seconds were optimal. In addition, based on corrosion resistance observations, the electrodes are quite suitable for long-term studies.

Adolescent males with urogenital anomalies: Their body image and psychosexual development.

Three midadolescent males with major congenital urogenital anomalies and multiple surgical repairs are described. Each patient had suffered repeated profound insults to body image concepts and gender identity from infancy onward, now clearly reflected in his psychosocial behavior and in figure drawings. Of major importance is the total lack at any time of counseling and emotional support as a part of comprehensive management. A plea is made for awareness of the psychological effects of such disorders and the need for long-range therapeutic planning from early childhood through adolescence to develop sound compensatory modes of coping with this stress.