Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Antimicrob Agents Chemother ; 49(5): 1915-26, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15855514

RESUMO

Global gene expression patterns of Bacillus subtilis in response to subinhibitory concentrations of protein synthesis inhibitors (chloramphenicol, erythromycin, and gentamicin) were studied by DNA microarray analysis. B. subtilis cultures were treated with subinhibitory concentrations of protein synthesis inhibitors for 5, 15, 30, and 60 min, and transcriptional patterns were measured throughout the time course. Three major classes of genes were affected by the protein synthesis inhibitors: genes encoding transport/binding proteins, genes involved in protein synthesis, and genes involved in the metabolism of carbohydrates and related molecules. Similar expression patterns for a few classes of genes were observed due to treatment with chloramphenicol (0.4x MIC) or erythromycin (0.5x MIC), whereas expression patterns of gentamicin-treated cells were distinct. Expression of genes involved in metabolism of amino acids was altered by treatment with chloramphenicol and erythromycin but not by treatment with gentamicin. Heat shock genes were induced by gentamicin but repressed by chloramphenicol. Other genes induced by the protein synthesis inhibitors included the yheIH operon encoding ABC transporter-like proteins, with similarity to multidrug efflux proteins, and the ysbAB operon encoding homologs of LrgAB that function to inhibit cell wall cleavage (murein hydrolase activity) and convey penicillin tolerance in Staphylococcus aureus.


Assuntos
Antibacterianos/farmacologia , Bacillus subtilis/genética , Inibidores da Síntese de Proteínas/farmacologia , Bacillus subtilis/efeitos dos fármacos , Bacillus subtilis/metabolismo , Metabolismo dos Carboidratos , Proteínas de Transporte/metabolismo , Cloranfenicol/farmacologia , Eritromicina/farmacologia , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Gentamicinas/farmacologia , Hibridização In Situ , Lipoproteínas/metabolismo , Análise de Sequência com Séries de Oligonucleotídeos , Ligação Proteica , RNA Bacteriano/biossíntese , Reação em Cadeia da Polimerase Via Transcriptase Reversa
2.
J Bacteriol ; 186(13): 4159-67, 2004 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15205417

RESUMO

Natural isolates of Bacillus subtilis exhibit a robust multicellular behavior known as swarming. A form of motility, swarming is characterized by a rapid, coordinated progression of a bacterial population across a surface. As a collective bacterial process, swarming is often associated with biofilm formation and has been linked to virulence factor expression in pathogenic bacteria. While the swarming phenotype has been well documented for Bacillus species, an understanding of the molecular mechanisms responsible remains largely isolated to gram-negative bacteria. To better understand how swarming is controlled in members of the genus Bacillus, we investigated the effect of a series of gene deletions on swarm motility. Our analysis revealed that a strain deficient for the production of surfactin and extracellular proteolytic activity did not swarm or form biofilm. While it is known that surfactin, a lipoprotein surfactant, functions in swarming motility by reducing surface tension, this is the first report demonstrating that general extracellular protease activity also has an important function. These results not only help to define the factors involved in eliciting swarm migration but support the idea that swarming and biofilm formation may have overlapping control mechanisms.


Assuntos
Bacillus subtilis/fisiologia , Endopeptidases/fisiologia , Bacillus subtilis/enzimologia , Bacillus subtilis/genética , Sequência de Bases , Biofilmes , Meios de Cultura , Flagelos/fisiologia , Dados de Sequência Molecular , Movimento
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...