RESUMO
As a contribution to the European Scientists Sequencing Arabidopsis (BIOTECH ESSA) project, a contig of almost 40kb has been sequenced at the extreme top of chromosome 1, around the Arabidopsis thaliana gene coding for a member of the 1-aminocyclopropane-1-carboxylate synthesis gene family. The region contains, besides the ACS1 gene itself, 10 putative genes, all new for Arabidopsis. Among these are three genes encoding kinases, a late embryogenesis-abundant protein, a MADS box-containing protein, a dehydrogenase, and a Myb-related transcription factor. In addition, six cDNAs have been sequenced that correspond to this region.
Assuntos
Arabidopsis/genética , Cromossomos/genética , DNA de Plantas/genética , Proteínas Proto-Oncogênicas c-myb , Arabidopsis/química , Proteínas de Arabidopsis , Mapeamento Cromossômico , Clonagem Molecular , DNA de Plantas/química , Proteínas de Ligação a DNA/genética , Expressão Gênica/genética , Genes de Plantas/genética , Genoma de Planta , Proteínas de Domínio MADS , Dados de Sequência Molecular , Oxirredutases/genética , Fosfotransferases/genética , Proteínas de Plantas/genética , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Fatores de Transcrição/genéticaRESUMO
Three genomic clones were isolated from a size-selected pig DNA library by hybridization with a DNA-fingerprint probe. Analysis at the sequence level revealed that all three clones contain interrupted stretches of triplet repeats mainly composed of CAC and CAT triplets. Evaluation of the corresponding loci for polymorphism by Southern blot hybridization showed considerable length variation. For two loci the polymorphism was also demonstrated by polymerase chain reaction (PCR) amplification. The PiGMaP reference pedigree was typed for all three loci.
Assuntos
Genoma , Polimorfismo Genético , Sequências Repetitivas de Ácido Nucleico , Suínos/genética , Animais , Sequência de Bases , Clonagem Molecular , Impressões Digitais de DNA , Sondas de DNA , Elementos de DNA Transponíveis , Biblioteca Gênica , Dados de Sequência Molecular , Mutagênese Insercional , Reação em Cadeia da Polimerase/métodosRESUMO
Genomic and cDNA clones for three inflorescence-specific genes from Arabidopsis thaliana were isolated and characterized. The genes are tandemly organized in the genome on a 10 kb fragment. The expression of these genes is coordinately regulated in a developmental and organ-specific pattern. They are expressed predominantly in anthers at the later stage of flower development. The primary structure of the encoded gene products exhibits comparable features consisting of a hydrophobic domain at the N-terminal region followed by repeated glycine-rich motifs. Little homology is observed either between the glycine-rich domain of the three genes or with previously described glycine-rich proteins from other plant species.
