[Proposal of a new methodology: tridimensional colposcopy (3D) of the lower genital tract]. / Proposition d'une nouvelle technique : la colposcopie 3D.

The combination of a colposcope to a computer with a 3D video adapter and a dedicated graphics card can get with a specialized 3D visualization deferred delayed colposcopic imaging software. The 3-dimensional images allow the observation of new aspects of epithelial surfaces from different angles which is possible to visualize with this technology alone.

[Investigation of Trichomonas vaginalis through different methodologies during pregnancy]. / Investigación de Trichomonas vaginalis durante el embarazo mediante diferentes metodologías.

The aim of this study was to conduct a survey regarding the prevalence of trichomoniasis in pregnant patients and to evaluate the utility of different diagnostic methods. Two hundred and twenty three vaginal swab specimens from pregnant women were prospectively examined. Trichomonas vaginalis was investigated by various microscopic examinations, solid culture medium and liquid culture medium. The sensitivity and specificity of microscopy were evaluated by considering both culture media as the "gold standards". The prevalence of T. vaginalis obtained by both culture media (liquid plus solid media) was 4.5% (10/223). The prevalence of T. vaginalis obtained by direct smear, May-Grunwald Giemsa staining, sodium acetate-acetic acid-formalin (SAF)/Methylene blue staining-fixing technique, solid medium and liquid medium was 1.3%, 1.8%, 1.8% and 4.5%, respectively. The sensitivity of the direct smear was 30 %, but for the May-Grunwald Giemsa staining and the SAF/Methylene blue staining-fixing technique was 40%. Considering the three microscopic examinations altogether, the sensitivity rose to 50% and the specificity was 100% for all of them. The solid medium detected only 50% of the positive cases; the liquid medium detected 100%. Due to the low sensitivity obtained with microscopy in asymptomatic pregnant patients, we recommend the use of the liquid medium during pregnancy, in order to provide an early treatment.

Investigación de Trichomonas vaginalis durante el embarazo mediante diferentes metodologías / Investigation of Trichomonas vaginalis through different methodologies during pregnancy

Los objetivos del trabajo fueron conocer la prevalencia de tricomonosis en pacientes embarazadas y evaluar la utilidad de diferentes metodologías para su diagnóstico. Se estudiaron prospectivamente 223 mujeres embarazadas. Trichomonas vaginalis se investigó utilizando distintos exámenes microscópicos, cultivo en medio sólido y medio líquido. Se evaluó la sensibilidad y especificidad de la microscopía considerando a los cultivos en ambos medios como método de referencia. La prevalencia del parásito obtenida por cultivo (medio líquido más medio sólido) fue de 4,5% (10/223) siendo la detección por examen en fresco, coloración de May-Grunwald Giemsa, fresco con solución acética formolada (SAF)/azul de metileno y por cultivo en medio sólido y líquido de 1,3%, 1,8%, 1,8% y 4,5% respectivamente. La sensibilidad del examen en fresco fue 30%, para el May-Grunwald Giemsa y el SAF/azul de metileno fue 40%. Utilizando conjuntamente los tres exámenes microscópicos, la sensibilidad se elevó al 50% y la especificidad fue 100% para todos los exámenes microscópicos. El cultivo en medio líquido detectó el 100% de los casos positivos , mientras que el medio sólido sólo el 50%. Por la baja sensibilidad de la microscopía para T. vaginalis, en embarazadas asintomáticas recomendamos la utilización del cultivo en medio líquido durante el embarazo, para instaurar un tratamiento precoz.

The aim of this study was to conduct a survey regarding the prevalence of trichomoniasis in pregnant patients and to evaluate the utility of different diagnostic methods. Two hundred and twenty three vaginal swab specimens from pregnant women were prospectively examined. Trichomonas vaginalis was investigated by various microscopic examinations, solid culture medium and liquid culture medium. The sensitivity and specificity of microscopy were evaluated by considering both culture media as the "gold standards". The prevalence of T. vaginalis obtained by both culture media (liquid plus solid media) was 4.5% (10/223). The prevalence of T. vaginalis obtained by direct smear, May-Grunwald Giemsa staining, sodium acetate-acetic acid-formalin (SAF)/Methylene blue staining-fixing technique, solid medium and liquid medium was 1.3%, 1.8%, 1.8% and 4.5%, respectively. The sensitivity of the direct smear was 30 %, but for the May- Grunwald Giemsa staining and the SAF/Methylene blue staining-fixing technique was 40%. Considering the three microscopic examinations altogether, the sensitivity rose to 50% and the specificity was 100% for all of them. The solid medium detected only 50% of the positive cases; the liquid medium detected 100%. Due to the low sensitivity obtained with microscopy in asymptomatic pregnant patients, we recommend the use of the liquid medium during pregnancy, in order to provide an early treatment.

Diagnostic value of the papanicolaou smear in lower genital tract infections during pregnancy.

OBJECTIVES: To evaluate the diagnostic value of the Pap smear in lower genital tract infections during pregnancy. MATERIALS AND METHODS: A total of 190 pregnant women were examined in the Lower Genital Tract and Colposcopy clinics of the Obstetrics Division at the University Hospital de Clínicas "José de San Martín" of University of Buenos Aires from April to August 1997. Samples were taken from the vaginal fornix for microbiologic study and from the exocervix for Pap smear. Both the sensitivity and specificity test of the Pap smear for diagnosing lower genital tract infections were calculated considering the microbiologic study as the reference standard. RESULTS: The sensitivity of the Pap smear for Candida spp., bacterial vaginosis and Trichomonas vaginalis compared with microbiologic study was 50, 81, and 78, respectively. The specificity for the same study was 96%, 93%, and 100%, respectively. CONCLUSIONS: Pap smear for diagnosing lower genital tract infections is useful when positive results are found for these infections because of its high specificity.

Colposcopia digital o computadorizada / Digital or computarized colposcopy

Un nuevo método colposcópico que utiliza un sistema computarizado para obtener imágenes cervicales digitalizadas, es descripto en este trabajo. Las imágenes son excelentes, pueden ser incorporadas a la memoria del equipo y permite, si es necesario, su reprodución. Creemos que es una herramienta útil para el diagnóstico y la investigación en el campo de la patología cervical (AU)

Colposcopia digital o computadorizada / Digital or computarized colposcopy

Un nuevo método colposcópico que utiliza un sistema computarizado para obtener imágenes cervicales digitalizadas, es descripto en este trabajo. Las imágenes son excelentes, pueden ser incorporadas a la memoria del equipo y permite, si es necesario, su reprodución. Creemos que es una herramienta útil para el diagnóstico y la investigación en el campo de la patología cervical