RESUMO
A boy with X-linked agammaglobulinemia experienced progressive global motor decline, cerebellar syndrome, and epilepsy. All standard polymerase chain reactions for neurotropic viruses were negative on cerebrospinal fluid and brain biopsy. Next-generation sequencing allowed fast identification of a new astrovirus strain (HAstV-VA1/HMO-C-PA), which led to tailor the patient's treatment, with encouraging clinical monitoring over 1 year.
Assuntos
Agamaglobulinemia/complicações , Infecções por Astroviridae/tratamento farmacológico , Infecções por Astroviridae/virologia , Astroviridae/genética , Encefalite Viral/tratamento farmacológico , Encefalite Viral/virologia , Doenças Genéticas Ligadas ao Cromossomo X/complicações , Adolescente , Agamaglobulinemia/tratamento farmacológico , Anti-Inflamatórios/uso terapêutico , Antivirais/administração & dosagem , Antivirais/uso terapêutico , Astroviridae/classificação , Astroviridae/isolamento & purificação , Infecções por Astroviridae/diagnóstico , Doenças Cerebelares/tratamento farmacológico , Doenças Cerebelares/etiologia , Encefalite Viral/diagnóstico , Epilepsia/tratamento farmacológico , Epilepsia/etiologia , Doenças Genéticas Ligadas ao Cromossomo X/tratamento farmacológico , Genoma Viral , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Masculino , Análise de Sequência de RNARESUMO
Aedes aegypti is responsible for the transmission of arboviruses. The Yellow Fever, Dengue and Chikungunya viruses are transmitted to the vertebrate host by injection of infected saliva during the blood meal of its vectors. Saliva contains different components with various biochemical activities; anti-hemostatic, angiogenic, inflammatory, and immunomodulatory. This work compares the sialomes of three Ae. aegypti colonies (Rockefeller, PAEA, and Formosus), where the repertoire of salivary proteins from these colonies was analyzed by a proteomic approach. This study indicated that major proteins were detectable in the three colonies. However, differences in the abundance of some saliva proteins have been observed between the three Ae. aegypti colonies.