Paenibacillus farraposensis sp. nov., isolated from a root nodule of Arachis villosa.

Strain UY79T was isolated from a root nodule of Arachis villosa, collected at the Esteros de Farrapos National Park, Río Negro, Uruguay. Cells were non-motile Gram-variable rods with central to subterminal oval to ellipsoidal endospores that swell the sporangia. Growth was observed in the range of 15-42 °C (optimum, 30 °C), pH 5.0-9.0 (optimum, pH 7.0-8.0) and with up to 3 % (w/v) NaCl (optimum, 1-2 %). Strain UY79T was facultative anaerobic, catalase-positive and oxidase-negative. According to the results of 16S rRNA gene sequence analysis, UY79T belongs to the genus Paenibacillus and is closely related to P. ottowii MS2379T, P. peoriae BD-57T, P. polymyxa ATCC 842T and P. brasilensis PB172T, exhibiting 99.4, 99.0, 99.0 and 98.9% sequence identity, respectively. Average nucleotide identity and digital DNA-DNA hybridization values with the most closely related type strains were 74.3-88.6% and 38.2-48.7 %, respectively. Major fatty acids (>10 %) were anteiso-C15:0, iso-C15:0, and C16 : 0. Menaquinones MK-7 and MK-6 were the only isoprenoid quinones detected. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid. Spermidine was the predominant polyamine. The DNA G+C content based on the draft genome sequence was 46.34 mol%. Based on the current polyphasic study, UY79T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus farraposensis sp. nov. is proposed. The type strain is UY79T (=CCM 9147T=CGMCC 1.19038T).

Paenibacillus sp. Strain UY79, Isolated from a Root Nodule of Arachis villosa, Displays a Broad Spectrum of Antifungal Activity.

A nodule-inhabiting Paenibacillus sp. strain (UY79) isolated from wild peanut (Arachis villosa) was screened for its antagonistic activity against diverse fungi and oomycetes (Botrytis cinerea, Fusarium verticillioides, Fusarium oxysporum, Fusarium graminearum, Fusarium semitectum, Macrophomina phaseolina, Phomopsis longicolla, Pythium ultimum, Phytophthora sojae, Rhizoctonia solani, Sclerotium rolfsii, and Trichoderma atroviride). The results obtained show that Paenibacillus sp. UY79 was able to antagonize these fungi/oomycetes and that agar-diffusible compounds and volatile compounds (different from HCN) participate in the antagonism exerted. Acetoin, 2,3-butanediol, and 2-methyl-1-butanol were identified among the volatile compounds produced by strain UY79 with possible antagonistic activity against fungi/oomycetes. Paenibacillus sp. strain UY79 did not affect symbiotic association or growth promotion of alfalfa plants when coinoculated with rhizobia. By whole-genome sequence analysis, we determined that strain UY79 is a new species of Paenibacillus within the Paenibacillus polymyxa complex. Diverse genes putatively involved in biocontrol activity were identified in the UY79 genome. Furthermore, according to genome mining and antibiosis assays, strain UY79 would have the capability to modulate the growth of bacteria commonly found in soil/plant communities. IMPORTANCE Phytopathogenic fungi and oomycetes are responsible for causing devastating losses in agricultural crops. Therefore, there is enormous interest in the development of effective and complementary strategies that allow the control of the phytopathogens, reducing the input of agrochemicals in croplands. The discovery of new strains with expanded antifungal activities and with a broad spectrum of action is challenging and of great future impact. Diverse strains belonging to the P. polymyxa complex have been reported to be effective biocontrol agents. Results presented here show that the novel discovered strain of Paenibacillus sp. presents diverse traits involved in antagonistic activity against a broad spectrum of pathogens and is a potential and valuable strain to be further assessed for the development of biofungicides.

Diabetic ankle fracture complications: a meta-analysis.

INTRODUCTION: This meta-analysis aimed to review complication rates following the treatment of an ankle fracture in diabetic patients and to early detect the subgroup of patients at potential risk in order to minimise this complication rate. METHODS: A search of 3 databases was performed for studies published till March 2018. Twelve studies met the eligibility criteria for further statistical analysis. An odds ratio (OR) with a 95% confidence interval (95% CI) for each complication was calculated between the diabetic and non-diabetic groups. RESULTS: The overall complication risk after ankle fracture was twice as high in diabetes mellitus (DM) than non-diabetes mellitus (non-DM) patients (OR 1.9, 95%CI: 1.7-2.03). This risk was considerably higher with surgery versus non-surgical treatment (OD 3.7, 95%CI: 2.3-6.2). The risk of infection was 3 times higher in DM than in non-DM patients (OR 3.4, 95%CI: 2.9-9.8). The complication rate was even higher in patients with advanced DM (OR 8.4, 95%CI: 2.9-24.5). CONCLUSION: This meta-analysis provides evidence that diabetic patients are at a greater risk of complication after an ankle fracture.

Labial dystonia after facial and trigeminal neuropathy controlled with a maxillary splint.

A 27-year-old woman with bruxism suffered a spider bite (Loxosceles rufescens) on the left cheek that caused severe local cellulitis, facial palsy, and painful hyperesthesia over the two lower trigeminal nerve divisions. Facial but not trigeminal neuropathy improved, and she developed a labial dystonia that only corrected while pressing the right medial incisor. A specially designed maxillary splint that continuously pressed it suppressed both dystonia and related spontaneous firing of motor unit potentials in electromyography. Overstimulation of the contralateral trigeminal territory possibly compensated for the altered left trigeminal nerve input, balanced proprioceptive influences at the central inhibitory-excitatory circuitry, and controlled dystonia.

Estudio comparativo entre el valor del INR y la determinación del tiempo de protrombina mediante el coagulómetro portátil Coaguchek® en el tratamiento dental del paciente anticoagulado / A comparative study between INR and the determination of prothrombin time with the Coaguchek® portable coagulometer in the dental treatment of anticoagulated patients

Objetivos: Comprobar la utilidad del coagulómetro portátil Coaguchek® como método para determinar el INR en la clínica dental. Diseño del estudio: En 88 pacientes anticoagulados con acenocumarol (Sintrom®) por patología trombótica se hicieron 139 determinaciones sanguíneas del INR en el laboratorio según el procedimiento habitual (Muestra 1), posteriormente y antes de nuestra actuación dental se efectuó otra determinación del INR, pero en esta ocasión mediante el Coaguchek® (Muestra 2).Seguidamente comparamos ambas determinaciones para valorar si existían diferencias significativas aplicando la "t de Student" para datos apareados y medidas de regresión. Resultados: La media de INR tomada en la muestra 1 era de 2,31 ñ 0,81, mientras que la hallada en la muestra 2 con el Coaguchek® fue de 2,28 ñ 0,82. Al compararlas comprobamos que no existían diferencias significativas entre ambas ya que obtuvimos una t= 0,98 y una p= 0,32. Asimismo ambas mostraron una relación estadística significativa manifestada por una R= 0,929 y p< 0,01. Conclusiones: El coagulómetro portátil Coaguchek® es un instrumento válido para determinar el INR del paciente anticoagulado y constituye un método eficaz, en manos del odontólogo, para poder realizar el tratamiento ambulatorio de estos pacientes (AU)

Férulas protectoras para la prevención de osteorradionecrosis en pacientes tratados con braquiterapia / Spacers for preventing osteoradionecrosis in patients treated with interstital brachytherapy

Se realiza en este trabajo una exposición de las principales características que se deben tener en cuenta a la hora de confeccionar y diseñar este tipo de prótesis, así como una serie de recomendaciones para su elaboración, colocación y ajuste Intentamos conseguir unas férulas que nos aseguren una máxima eficacia en la reducción de la dosis de radiación absorbida por periostio y hueso, que resulten de fácil manipulación por personal no experto, que interfieran lo menos posible con las intervenciones realizadas por los radioterapeutas y que aseguren un mínimo de confort al paciente (AU)