Association between screen time and accelerometer-measured 24-h movement behaviors in a sample of Brazilian adolescents.

OBJECTIVES: Different screen time activities may be related to sleep, physical activity, and sedentary behavior. The objective was to examine the association between self-reported screen time activities and accelerometer-measured 24-h movement behaviors. STUDY DESIGN: This was a cross-sectional study. METHODS: Adolescents' (n = 718, 50.4% girls, 16 years) sleep duration, sedentary behavior, light-intensity physical activity (LPA), and moderate-to-vigorous physical activity (MVPA) were estimated with wrist-worn accelerometry. Time spent on screen time activities related to studying, working, watching videos, playing video games, and using social media was self-reported. Multilevel linear regressions were used to test the association between screen time with sleep, sedentary behavior, and physical activity. RESULTS: Boys and girls slept 6.4 and 6.7 h per night, spent 10.4 and 10.1 h/d in sedentary behavior, spent 4.0 and 4.4 h/d in LPA, and spent 34.7 and 29.2 min/d in MVPA, respectively. Studying was inversely related to LPA and MVPA. Working was inversely related to sleep and positively related to LPA. Watching videos was associated with lower LPA and MVPA. For boys, videogames were associated with increased sedentary behavior and lower LPA and MVPA. For girls, studying and/or using social media were associated with lower LPA and MVPA. CONCLUSIONS: Indicators of screen time were associated with different accelerometer-measured 24-h movement behaviors in this sample of Brazilian adolescents.

Steam Sterilization of Equine Bone Block: Morphological and Collagen Analysis.

INTRODUCTION: The use of equine bone blocks is widely reported for bone augmentation techniques. The block must be shaped according to the form of the defect that should be regenerated. The shaping could be performed by hand before or during the surgery, in a sterile ambient, or using a CNC milling machine that could not be sterile. The aim of our study was to evaluate if a steam sterilization could provide a medical grade sterilization of the blocks and to evaluate if bone microstructure and collagen structures change after different steam sterilization protocols provided by mainstream autoclave. MATERIALS AND METHOD: Two blocks of equine bone were divided into 16 samples. 1 sample was used as control and not submitted to any treatment. 15 samples were infected with a Streptococcus faecalis bacterial culture. The samples were singularly packed, randomly divided into 3 groups, and submitted to autoclave sterilization on the same device. The groups were submitted to a sterilization cycle (Gr. A: 121°C, 1,16 bar for 20'; Gr. B:134°C, 2,16 bar for 4'; Gr. C: 134°C, 2,16 bar for 3.30 min.). 2 samples for each group were evaluated for the sterility. 3 samples for each group were observed at SEM to notice the macro- and microstructure modification and to confocal microscope to observe the collagen. RESULTS: All samples were sterile. The SEM evaluation showed, in all groups, a preserved morphological structure. Confocal microscope evaluation shows that the collagen structure appears to be more uniform and preserved in group C. CONCLUSION: Data show that autoclave steam sterilization could be reliable to obtain sterilization of equine bone blocks.

Mercury (Hg) in fish consumed by the local population of the Jaguaribe River lower basin, Northeast Brazil.

The knowledge of Hg concentrations in fish is of considerable interest since these organisms are a major source of protein to coastal human populations and fishing communities. The main source of human exposure to Hg contamination occurs through the consumption of fish. In this paper, we compare Hg concentration in 13 fish species from Jaguaribe River lower basin and an adjacent coastal region in the northeastern coast of Brazil. We sampled fish from three stretches of the river: fluvial, estuarine, and marine regions. We tested the hypothesis that Hg concentration in muscle tissue vary according to species, location, and trophic level. Significant differences were observed among species and trophic level, but these could not be observed among the regions studied. As expected, the highest concentrations were observed in carnivorous fish (5.6-107.5; 26.9 ± 18.8 ng g(-1)). Hg concentrations observed in this study are similar to those observed in regions of low environmental contamination. We estimated Hg intake to vary between 0.02 and 0.22 ng Hg kg body weight(-1) week(-1), for the average body weight of 56.7 kg, which was considered as low exposure and therefore, a low risk to consumers of fish from the regions studied.

Mercury in indigenous, introduced and farmed fish from the semiarid region of the Jaguaribe river Basin, NE Brazil.

Indigenous, introduced and farmed fish species were collected from the Jaguaribe Basin, NE Brazil to assess total and methyl mercury concentrations in muscle tissue and to determine its relationship with fish origin. The results obtained from introduced species were compared to the Hg content of their original area of occurrence, which is located in the Amazon region. Farmed and wild individuals of Oreochromis niloticus (tilapia) were also compared. Introduced species presented lower Hg contents compared to their Amazonian counterparts. Wild individuals of O. niloticus also presented higher Hg contents than farmed individuals with similar sizes. However, methyl Hg concentrations were not significantly different between the two groups. Total Hg and methyl Hg concentrations were higher in carnivorous species, especially those from the Amazon region, and surpassed the Brazilian guidelines for fish consumption. Based on their Hg content, Jaguaribe basin fish do not pose a significant threat to human consumption.

Mercury distribution in a mangrove tidal creek affected by intensive shrimp farming.

In this study, the Hg distributions in water and sediments from a mangrove creek that receives intensive shrimp farming effluents were determined. The average dissolved and particulate Hg concentrations in the water varied from 3.1 to 9.2 ng L(-1) and from 4.4 to 9.4 ng L(-1), respectively. However, the Hg concentrations in the suspended particulate matter and the bottom sediments varied from 95.4 to 115.7 ng g(-1) and from 1.6 to 10.3 ng g(-1), respectively. A Ward quadratic distance cluster analysis based on the Hg concentrations and hydro- and geochemical parameters (oxygen, salinity, temperature, pH, and organic matter and aluminum content) showed the effects of shrimp farming effluents on the Hg distribution pattern. Furthermore, these results were supported by the Hg distribution in the sediment cores. This study emphasizes the necessity of including Hg as a potential pollutant when monitoring the environmental impacts of intensive shrimp farming.

Mercury emission factors from intensive shrimp aquaculture and their relative importance to the Jaguaribe River Estuary, NE Brazil.

This study estimated Hg emission factors (EFs) and total Hg loading to the Jaguaribe Estuary, NE Brazil, from intensive shrimp farming, and compares this with other local anthropogenic activities. The EF reached 83.5 mg ha(-1) cycle(-1) (about 175 mg ha(-1) year(-1)), resulting in an annual Hg load to the estuary of 0.35 kg. The calculated EF is comparable to Hg EFs from urban wastewaters (200 mg ha(-1)) and solid waste disposal (400 mg ha(-1) year(-1)) from cities located in the estuary's basin. However, due to the smaller area of aquaculture (2,010 ha), total annual loads are much lower than from these other sources (75 and 150 kg year(-1), respectively). Since shrimp farming effluents are released directly into the estuary, the estimated high EF raises environmental concerns with this expanding industry, suggesting the inclusion of this element in ongoing environmental monitoring programs.