Fusobacterium nucleatum and Prevotella in women with periodontitis and preterm birth.

BACKGROUND: Studies try to explain the hypothesis that maternal periodontitis may be associated with preterm birth. MATERIAL AND METHODS: This is a case-control study with 120, 40 cases (gestational age <37 weeks) and 80 controls (gestational age ≥37 weeks), that were submitted to the clinical periodontal examination and subgingival biofilm collection. Bacterial DNA of subgingival biofilm was performed and processed by qPCR. RESULTS: Periodontitis was statistically significant in the Case group (35%) when compared to the Control group (11.2%) and Gingival Bleeding Index (GBI), sites with PS ≥ 4mm and sites with CAL ≥ 5mm were statistically higher in the Case group (p < 0.05). The proportions of Pi (p = 0.026) and Fn (p = 0.041) of subgingival biofilm were higher in the Case group. A greater number of sites with PS ≥ 4mm (r = -0.202; p = 0.026) and CAL ≥ 5mm (r = -0.322; p < 0.001) were correlated to lower gestational age. CONCLUSIONS: Periodontitis, preterm delivery, and/or low birth weight may have a possible relationship based on clinical parameters and the ratio of Pi and Fn at periodontal sites.

First molecular evidence of frogs as a food source for sand flies (Diptera: Phlebotominae) in Brazilian caves.

Genus and species of phlebotomine sand flies have been recorded and described in caves in Brazil, but no study has provided the food source used by sand flies in these environments. Herein, we identified the blood source used by sand fly species in caves located at "Quadrilátero Ferrífero" (QF), Minas Gerais state. Specimens were manually collected near or on anurans inside ferruginous caves in Serra do Gandarela National Park and Serra do Rola Moça State Park. Males and females were placed in vials with 70% alcohol and stored at -10°C. Females engorged, after specific identification, had DNA extracted and followed for PCR amplification using specific primers. Sequencing was analyzed in the GenBank and Barcode of Life. A total of 198 specimens were collected (107 females and 91 males), all of them belonging to species Sciopemyia aff. microps (88.89%), Sciopemyia sordellii (10.61%), or Martinsmyia oliveirai (0.50%). When it comes to the females, 89 were S. aff. microps and 18 S. sordellii. Nineteen engorged females of S. aff. microps were analyzed and most of them (n=18) presented blood from Bokermannohyla martinsi and one contained blood from Scinax fuscovarius. The blood present in engorged females of S. sordellii (n=4) was from B. martinsi. Sciopemyia genus specimens are commonly found in collections carried out inside natural caves, but this was the first study to prove that females of this genus feed on cold-blooded animals in nature. HIGHLIGHTS: • Here we proved that sand flies feed in cold-blooded animals in in Brazilian caves. • Females of the Sciopemyia genus were for the first time found feeding in natural habitats. • Anurans of the family Hylidae were identified as source by molecular analyzes. • Insect bloodmeal identification can help assessing the fauna in several biomes. • This is the first record of S. aff. microps in caves of Brazil.

Pregnancy swimming prevents early brain mitochondrial dysfunction and causes sex-related long-term neuroprotection following neonatal hypoxia-ischemia in rats.

Neonatal hypoxia-ischemia (HI) is a major cause of cognitive impairments in infants. Antenatal strategies improving the intrauterine environment can have high impact decreasing pregnancy-derived intercurrences. Physical exercise alters the mother-fetus unity and has been shown to prevent the energetic challenge imposed by HI. This study aimed to reveal neuroprotective mechanisms afforded by pregnancy swimming on early metabolic failure and late cognitive damage, considering animals' sex as a variable. Pregnant Wistar rats were submitted to daily swimming exercise (20' in a tank filled with 32 °C water) during pregnancy. Neonatal HI was performed in male and female pups at postnatal day 7. Electron chain transport, mitochondrial mass and function and ROS formation were assessed in the right brain hemisphere 24 h after HI. From PND45, reference and working spatial memory were tested in the Morris water maze. MicroPET-FDG images were acquired 24 h after injury (PND8) and at PND60, following behavioral analysis. HI induced early energetic failure, decreased enzymatic activity in electron transport chain, increased production of ROS in cortex and hippocampus as well as caused brain glucose metabolism dysfunction and late cognitive impairments. Maternal swimming was able to prevent mitochondrial dysfunction and to improve spatial memory. The intergenerational effects of swimming were sex-specific, since male rats were benefited most. In conclusion, maternal swimming was able to affect the mitochondrial response to HI in the offspring's brains, preserving its function and preventing cognitive damage in a sex-dependent manner, adding relevant information on maternal exercise neuroprotection and highlighting the importance of mitochondria as a therapeutic target for HI neuropathology.

A new SYBR Green real-time PCR to detect SARS-CoV-2.

Phylogenetic analysis has demonstrated that the etiologic agent of the 2020 pandemic outbreak is a betacoronavirus named SARS-CoV-2. For public health interventions, a diagnostic test with high sensitivity and specificity is required. The gold standard protocol for diagnosis by the Word Health Organization (WHO) is RT-PCR. To detect low viral loads and perform large-scale screening, a low-cost diagnostic test is necessary. Here, we developed a cost-effective test capable of detecting SARS-CoV-2. We validated an auxiliary protocol for molecular diagnosis with the SYBR Green RT-PCR methodology to successfully screen negative cases of SARS-CoV-2. Our results revealed a set of primers with high specificity and no homology with other viruses from the Coronovideae family or human respiratory tract pathogenic viruses, presenting with complementarity only for rhinoviruses/enteroviruses and Legionella spp. Optimization of the annealing temperature and polymerization time led to a high specificity in the PCR products. We have developed a more affordable and swift methodology for negative SARS-CoV-2 screening. This methodology can be applied on a large scale to soften panic and economic burden through guidance for isolation strategies.

Differential glucose and beta-hydroxybutyrate metabolism confers an intrinsic neuroprotection to the immature brain in a rat model of neonatal hypoxia ischemia.

Neonatal hypoxia ischemia (HI) is the main cause of newborn mortality and morbidity. Preclinical studies have shown that the immature rat brain is more resilient to HI injury, suggesting innate mechanisms of neuroprotection. During neonatal period brain metabolism experience changes that might greatly affect the outcome of HI injury. Therefore, the aim of the present study was to investigate how changes in brain metabolism interfere with HI outcome in different stages of CNS development. For this purpose, animals were divided into 6 groups: HIP3, HIP7 and HIP11 (HI performed at postnatal days 3, 7 and 11, respectively), and their respective shams. In vivo [18F]FDG micro positron emission tomography (microPET) imaging was performed 24 and 72 h after HI, as well as ex-vivo assessments of glucose and beta-hydroxybutyrate (BHB) oxidation. At adulthood behavioral tests and histology were performed. Behavioral and histological analysis showed greater impairments in HIP11 animals, while HIP3 rats were not affected. Changes in [18F]FDG metabolism were found only in the lesion area of HIP11, where a substantial hypometabolism was detected. Furthermore, [18F]FDG hypometabolism predicted impaired cognition and worst histological outcomes at adulthood. Finally, substrate oxidation assessments showed that glucose oxidation remained unaltered and higher level of BHB oxidation found in P3 animals, suggesting a more resilient metabolism. Overall, present results show [18F]FDG microPET predicts long-term injury outcome and suggests that higher BHB utilization is one of the mechanisms that confer the intrinsic neuroprotection to the immature brain and should be explored as a therapeutic target for treatment of HI.

Notch signaling in human iPS-derived neuronal progenitor lines from Focal Cortical Dysplasia patients.

Focal Cortical Dysplasia (FCD), a common type of Malformations of cortical development, may result from an early disturbance in the migration and final arrangement of the cortical architecture of immature neurons. FCD type II is now known to be due to a post-zygotic somatic mutation that involves the mTOR and AKT pathways. The aim of the present study was to investigate the possible differences in neurogenesis and neurodifferentiation of iPSCs (induced pluripotent stem cells) from fibroblasts of individuals affected by FCD type II (2) and normal individuals (2). iPSCs were generated from skin fibroblasts of FCD individuals and healthy individuals. The reprogramming was done through the fibroblasts exposure to viral vectors containing the OCT4, KLF4, SOX2, and c-MYC genes and the clones were characterized by immunohistochemistry. iPSCs were neurodifferentiated and analyzed at the 14th, 22nd and 35th days. We also analyzed the cerebral cortex tissue, fibroblasts and iPSCs cells from the individuals. Through qRT-PCR, the expression of 4 genes involved in Notch signaling process were quantified. In general, individuals with dysplasia presented increase and decrease in the relative quantification in the most genes analyzed compared to control individuals in all processes and study groups. We suggest that, during embryonic neurogenesis, the neural precursor cells of FCD type II individuals present increase and decrease in gene expression in the Notch signaling pathway causing cortical formation disorders and can be seen as a candidate for the developmental changes observed in the cerebral cortex of individuals with FCD type II. This altered gene expression may be related to brain formation with dysplasia.

The Bronchiectasis Severity Index and FACED score for assessment of the severity of bronchiectasis.

INTRODUCTION: Bronchiectasis (BC) is a multidimensional and etiologically diverse disease and, therefore, no single parameter can be used to determine its overall severity and prognosis. In this regard, two different validated scores are currently used to assess the severity of non-cystic fibrosis bronchiectasis (NCFB): the FACED score and the Bronchiectasis Severity Index (BSI). OBJECTIVE: To describe the etiology of NCFB and compare the results of the assessment of NCFB severity obtained via FACED and BSI scores. METHODS: Retrospective study of demographic and clinical data of a convenience sample of NCFB patients attending the Functional Breathing Re-adaptation appointment at the Pneumology B Unit, University Hospital Center of Coimbra. All patients underwent evaluation of the variables incorporated in the FACED score (FEV1% predicted, age, chronic colonization by Pseudomonas aeruginosa, radiological extent of the disease, and dyspnea) and in the BSI (age, body mass index, FEV1% predicted, hospitalization and exacerbations before study, dyspnea, chronic colonization by P. aeruginosa and other microrganisms, and radiological extent of the disease). Statistical analysis of the data was performed using Microsoft Excel® and IBM SPSS® v23. RESULTS: The sample included 40 patients, 22 females and 18 males, aged 39-87 years. Regarding the etiology of NCFB, we found: idiopathic (60%), post-infectious (20%), sequelae of pulmonary tuberculosis (12.5%) and primary immunodeficiency related (7.5%). According to the FACED score we found 20 patients (50%) with mild BC, 15 patients (37.5%) with moderate and 5 patients (12.5%) with severe BC. The frequency of patients with low, intermediate and high BSI was 13 (32.5%), 13 (32.5%) and 14 (35%), respectively in relation to derived BSI, Moreover, we observed a weak but statistically significant association between FACED and BSI scores: Fisher's exact test (p=0.004), tau-b de Kendall (0.469; p=0.001). The Kappa test (0.330; p=0.002) also shows us that there is 55% agreement between the two scales. CONCLUSION: There is a small but significant correlation between the two scales: a tendency is observed for patients to be classified with a higher BSI compared to the FACED score. This can be explained by the fact that BSI (and not FACED) evaluates parameters including BMI, hospitalization and exacerbations before study, chronic colonization by other microorganisms and development of cystic bronchiectasis. Further studies should address how these scores may impact clinical practice.

Likelihood analysis of the pMSSM11 in light of LHC 13-TeV data.

We use MasterCode to perform a frequentist analysis of the constraints on a phenomenological MSSM model with 11 parameters, the pMSSM11, including constraints from ∼ 36 /fb of LHC data at 13 TeV and PICO, XENON1T and PandaX-II searches for dark matter scattering, as well as previous accelerator and astrophysical measurements, presenting fits both with and without the ( g - 2 ) µ constraint. The pMSSM11 is specified by the following parameters: 3 gaugino masses M 1 , 2 , 3 , a common mass for the first-and second-generation squarks m q ~ and a distinct third-generation squark mass m q ~ 3 , a common mass for the first-and second-generation sleptons m ℓ ~ and a distinct third-generation slepton mass m τ ~ , a common trilinear mixing parameter A, the Higgs mixing parameter µ , the pseudoscalar Higgs mass M A and tan ß . In the fit including ( g - 2 ) µ , a Bino-like χ ~ 1 0 is preferred, whereas a Higgsino-like χ ~ 1 0 is mildly favoured when the ( g - 2 ) µ constraint is dropped. We identify the mechanisms that operate in different regions of the pMSSM11 parameter space to bring the relic density of the lightest neutralino, χ ~ 1 0 , into the range indicated by cosmological data. In the fit including ( g - 2 ) µ , coannihilations with χ ~ 2 0 and the Wino-like χ ~ 1 ± or with nearly-degenerate first- and second-generation sleptons are active, whereas coannihilations with the χ ~ 2 0 and the Higgsino-like χ ~ 1 ± or with first- and second-generation squarks may be important when the ( g - 2 ) µ constraint is dropped. In the two cases, we present χ 2 functions in two-dimensional mass planes as well as their one-dimensional profile projections and best-fit spectra. Prospects remain for discovering strongly-interacting sparticles at the LHC, in both the scenarios with and without the ( g - 2 ) µ constraint, as well as for discovering electroweakly-interacting sparticles at a future linear e + e - collider such as the ILC or CLIC.

Genetic diversity of Desmanthus sp accessions using ISSR markers and morphological traits.

Desmanthus is a genus of forage legumes with high nutritional value, productive potential, and ability to obtain nitrogen in association with diazotrophic bacteria. The use of accurate techniques for genotype identification and characterization is essential for breeding programs. Morphological markers are widely used to know the genetic diversity and the molecular markers are fundamental in these studies. We investigated the genetic diversity among Desmanthus sp genotypes in Pernambuco (Brazilian Northeast State), using morphological traits and ISSR markers. Morphological and molecular characterizations were performed in 18 and 26 accessions, respectively, in plants belonging to the germplasm bank of forage legumes of Universidade Federal Rural de Pernambuco (UFRPE), Academic Unit of Serra Talhada, PE, Brazil. Eight ISSR primers were selected, and 95 loci were generated, with polymorphism of 95.79%. The allele number observed was 1.958, where the effective number was 1.359, and the Nei diversity genetic index was 0.226. About the morphological markers, seed number was the variable that most contributed to the genetic divergence. A large amount of genetic diversity was observed among Desmanthus species, occurring spontaneously in Pernambuco, Brazil. Thus, the variability found in morphological and ISSR markers is importance for the improvement of Desmanthus spp. Our findings showed that 17L, 27L, 25F, 22F, 19S, 13Au, and 28G accessions could be used in breeding programs to explore the maximum genetic divergence.

Likelihood analysis of the minimal AMSB model.

We perform a likelihood analysis of the minimal anomaly-mediated supersymmetry-breaking (mAMSB) model using constraints from cosmology and accelerator experiments. We find that either a wino-like or a Higgsino-like neutralino LSP, [Formula: see text], may provide the cold dark matter (DM), both with similar likelihoods. The upper limit on the DM density from Planck and other experiments enforces [Formula: see text] after the inclusion of Sommerfeld enhancement in its annihilations. If most of the cold DM density is provided by the [Formula: see text], the measured value of the Higgs mass favours a limited range of [Formula: see text] (and also for [Formula: see text] if [Formula: see text]) but the scalar mass [Formula: see text] is poorly constrained. In the wino-LSP case, [Formula: see text] is constrained to about [Formula: see text] and [Formula: see text] to [Formula: see text], whereas in the Higgsino-LSP case [Formula: see text] has just a lower limit [Formula: see text] ([Formula: see text]) and [Formula: see text] is constrained to [Formula: see text] in the [Formula: see text] ([Formula: see text]) scenario. In neither case can the anomalous magnetic moment of the muon, [Formula: see text], be improved significantly relative to its Standard Model (SM) value, nor do flavour measurements constrain the model significantly, and there are poor prospects for discovering supersymmetric particles at the LHC, though there are some prospects for direct DM detection. On the other hand, if the [Formula: see text] contributes only a fraction of the cold DM density, future LHC [Formula: see text]-based searches for gluinos, squarks and heavier chargino and neutralino states as well as disappearing track searches in the wino-like LSP region will be relevant, and interference effects enable [Formula: see text] to agree with the data better than in the SM in the case of wino-like DM with [Formula: see text].

Likelihood analysis of supersymmetric SU(5) GUTs.

We perform a likelihood analysis of the constraints from accelerator experiments and astrophysical observations on supersymmetric (SUSY) models with SU(5) boundary conditions on soft SUSY-breaking parameters at the GUT scale. The parameter space of the models studied has seven parameters: a universal gaugino mass [Formula: see text], distinct masses for the scalar partners of matter fermions in five- and ten-dimensional representations of SU(5), [Formula: see text] and [Formula: see text], and for the [Formula: see text] and [Formula: see text] Higgs representations [Formula: see text] and [Formula: see text], a universal trilinear soft SUSY-breaking parameter [Formula: see text], and the ratio of Higgs vevs [Formula: see text]. In addition to previous constraints from direct sparticle searches, low-energy and flavour observables, we incorporate constraints based on preliminary results from 13 TeV LHC searches for jets + [Formula: see text] events and long-lived particles, as well as the latest PandaX-II and LUX searches for direct Dark Matter detection. In addition to previously identified mechanisms for bringing the supersymmetric relic density into the range allowed by cosmology, we identify a novel [Formula: see text] coannihilation mechanism that appears in the supersymmetric SU(5) GUT model and discuss the role of [Formula: see text] coannihilation. We find complementarity between the prospects for direct Dark Matter detection and SUSY searches at the LHC.

PRES with asymptomatic spinal cord involvement. Is this scenario more common than we know?

INTRODUCTION: Posterior reversible encephalopathy syndrome (PRES) is an entity characterized by neurologic symptoms such as headaches, altered mental status, seizures and visual changes, and it is associated with white matter vasogenic edema predominantly affecting the posterior occipital and parietal lobes of the brain. CASE REPORT: A 19-year-old patient developed PRES after the use of chemotherapy for a testicular teratocarcinoma and after the development of a blood pressure elevation. DISCUSSION: Few cases described the involvement of the spinal cord in this syndrome. In the majority of these cases, the spinal cord involvement was asymptomatic or with few symptoms of spinal cord disease.

Etnobotânica de plantas medicinais em duas comunidades do município de Picuí, Paraíba, Brasil / Ethnobotanic of medicinal plants in two communities in the municipality of Picuí, Paraíba, Brazil

RESUMO O presente trabalho realizou um levantamento etnobotânico sobre a utilização de plantas medicinais no município de Picuí, Seridó Oriental, Estado da Paraíba, Brasil. Foi analisada uma área urbana (bairro monte santo) e uma rural (sítio massapê). As informações etnobotânicas foram obtidas através de formulários semiestruturados, contendo características socioeconômicas dos entrevistados, características das plantas e seus usos terapêuticos. Para a entrevista foi selecionado o adulto responsável pela residência, “chefe de família”, e utilizou-se a técnica da “bola de neve”. Foram citadas 37 famílias botânicas, 59 gêneros e 64 espécies. As espécies mais citadas foram Vernonia condensata Baker e Chenopodium ambrosioides L. e a família mais indicada foi Fabaceae. O hábito predominante foi o herbáceo, seguido do arbóreo; as partes da planta mais utilizadas foram as folhas e as cascas do caule; os principais modos de preparo dos remédios foram os chás e a água e/ou sumo. A planta com maior CUPc foi Vernonia condensata Baker (41,67%), sendo também uma das plantas com maior FRt junto a Chenopodium ambrosioides L., ambas com 19,6%. Esses resultados demonstraram que as populações estudadas ainda usam plantas medicinais com frequência, entretanto, a maioria das plantas utilizadas não é nativa da caatinga.

ABSTRACT This study conducted an ethnobotanical survey on the use of medicinal plants in the municipality of Picuí, Seridó of Paraíba, Brazil. An urban area (neighborhood monte santo) and a rural area (massapê farm) were analysed. The ethnobotanical information was obtained through semi-structured questionary containing socioeconomic characteristics of the respondents, characteristics of plants and their therapeutic uses. We selected an adult which was the responsible adult of the residence “householder” for the interview and used the technique of “snowball”. 37 plant families, 59 genera and 64 species were cited. The species most frequently cited were Vernonia condensata Baker and Chenopodium ambrosioides L. and the most mentioned family was Fabaceae. The predominant habit was the herbaceous, followed by woody, the parts of the plant which were most used are the leaves and bark of the stem, the main modes of preparation of the medicines were showers and water and / or lushness. The plant with the highest CUPc was Vernonia condensata Baker (41.67%), and it was also one of the plants with greatest FRt along with Chenopodium ambrosioides L., both with 19.6%. These results demonstrated that the studied populations still use medicinal plants often, however, most of the plants used is not native to the caatinga.

Etnobotânica de espécies vegetais medicinais no tratamento de transtornos do sistema gastrointestinal / Ethnobotany of medicinal species in the treatment of gastrointestinal system disorders

RESUMO O estudo objetivou selecionar na comunidade Rio Urubueua de Fátima, Abaetetuba-Pará, as espécies vegetais utilizadas no tratamento de transtornos do sistema gastrointestinal, em determinado contexto de uso, associado a um conhecimento construído localmente. A pesquisa foi realizada com 35 informantes entre 28 e 93 anos, selecionados pelo método bola de neve. Os dados foram obtidos por observação participante e entrevistas semiestruturadas. Para a importância relativa das espécies vegetais, calculou-se a porcentagem de concordância quanto aos usos principais (CUP) e concordância quanto aos usos principais corrigida (CUPc). Os interlocutores indicaram várias receitas terapêuticas, e, destas, foram escolhidas as mais empregadas no tratamento da diarreia, por ser doença recorrente na comunidade. Foi investigado o potencial químico das plantas por meio de literatura científica e bancos de dados. “Boldo” e “Anoerá” apresentaram valor máximo de CUP (100%), enquanto a “Hortelã” obteve maior CUPc (87,5%). Das 79 espécies vegetais empregadas como medicinais, nove estão na lista do SUS. Os remédios são preparados exclusivamente sob a forma de chá, sendo a folha a parte da planta mais utilizada (65%). Para o tratamento de diarreia, 12 espécies utilizadas pelos interlocutores têm compostos químicos comprovados por literatura especializada. A comunidade de Rio Urubueua de Fátima faz uso das plantas medicinais para curar doenças, apropriando-se de conhecimentos obtidos, na maioria dos relatos, de seus antepassados. Estes saberes tradicionais contribuem no conhecimento cultural da região e na pesquisa e desenvolvimento de novos fármacos.

ABSTRACT This study aimed to select the plants used to treat disorders of the gastrointestinal system, in the Rio Urubueua community of Fatima, Abaetetuba, and Pará for their particular context of use, combined with local knowledge. The survey was conducted with 35 informants between the ages of 28 and 93, selected using the snowball method.Data was obtained by participant observation, semi-structured interviews and by non-specific induction. To show the relative importance of plant species, we calculated the percentage of agreement on the main uses (CUP) and agreement with regards to the corrected main uses (cUPC).The interlocutors indicated various therapeutic recipes, and from these, those most used in the treatment of diarrhea were chosen, due to having recurrent cases of this disease in the community. The chemical potential of plants was investigated using scientific documents and databases. “Boldo” and “Anoerá” showed a maximum CUP value (100%), while the “Hortelã” obtained a higher cUPC (87.5%). Of the 79 plant species used in traditional medicine in this community, nine are used by the national healthcare service(SUS). The drugs are prepared only in the form of tea, with the leaves being the most widely used plant parts (65%).For the treatment of diarrhea, the 12 species used by the interlocutors have chemical compositions that are supported by specialized literature. The Rio Urubueua community of Fatima makes use of medicinal plants to cure diseases, from knowledge obtained, in most cases, from their ancestors. This traditional knowledge contributes to the cultural knowledge of the region and to the research and development of new drugs.

Differential impact of chronic stress along the hippocampal dorsal-ventral axis.

Stress impacts differently in distinct brain regions. However, so far few studies have focused on the differential responses triggered by stressful stimuli on the intrinsic functional heterogeneity of the hippocampal axis. In this study, we assessed the functional and structural alterations caused by exposure to a chronic unpredictable stress (CUS) paradigm on the dorsal-ventral axis of the hippocampus. The morphological analysis demonstrated that CUS had opposite outcomes in the structure of the dorsal (DH) and ventral hippocampus (VH): whereas in the DH, stress triggered a volumetric reduction as a result of atrophy of CA3 and CA1 apical dendrites, in the VH there was an increase in hippocampal volume concurrent with the increase of CA3 apical dendrites. In parallel, electrophysiological data revealed that stress led to a decrease in VH LTD. In summary, the present work showed that stress impacts differently on the structure and function of the DH and VH which contributes to better understand the overall spectrum of the central effects of stress.

Optimization of biogas production from Sargassum sp. using a design of experiments to assess the co-digestion with glycerol and waste frying oil.

A design of experiments was adopted to assess the optimal conditions for methane production from the macroalgae Sargassum sp. co-digested with glycerol (Gly) and waste frying oil (WFO). Three variables were tested: % total solids of algae (%TSSargassumsp.), co-substrate concentration (gGly/WFOL(-1)), and co-substrate type (Gly or WFO). The biochemical methane potential (BMP) of Sargassum sp. was 181±1L CH4kg(-1) COD. The co-digestion with Gly and WFO increased the BMP by 56% and 46%, respectively. The methane production rate (k), showed similar behaviour as the BMP, increasing 38% and 19% with Gly and WFO, respectively. The higher BMP (283±18L CH4kg(-1) COD) and k (65.9±2.1L CH4kg(-1) CODd(-1)) was obtained in the assay with 0.5% TS and 3.0gGlyL(-1). Co-digestion with glycerol or WFO is a promising process to enhance the BMP from the macroalgae Sargassum sp.

Design of experiments to assess pre-treatment and co-digestion strategies that optimize biogas production from macroalgae Gracilaria vermiculophylla.

A design of experiments was applied to evaluate different strategies to enhance the methane yield of macroalgae Gracilaria vermiculophylla. Biochemical Methane Potential (BMP) of G. vermiculophylla after physical pre-treatment (washing and maceration) reached 481±9 L CH4 kg(-1) VS, corresponding to a methane yield of 79±2%. No significant effects were achieved in the BMP after thermochemical pre-treatment, although the seaweeds solubilisation increased up to 44%. Co-digestion with glycerol or sewage sludge has proved to be effective for increasing the methane production. Addition of 2% glycerol (w:w) increased the BMP by 18%, achieving almost complete methanation of the substrate (96±3%). Co-digestion of seaweed and secondary sludge (15:85%, TS/TS) increased the BMP by 25% (605±4 L CH4 kg(-1) VS) compared to the seaweed individual digestion.

Representative elementary length to measure soil mass attenuation coefficient.

With increasing demand for better yield in agricultural areas, soil physical property representative measurements are more and more essential. Nuclear techniques such as computerized tomography (CT) and gamma-ray attenuation (GAT) have been widely employed with this purpose. The soil mass attenuation coefficient (µ(s)) is an important parameter for CT and GAT analysis. When experimentally determined (µ(es)), the use of suitable sized samples enable to evaluate it precisely, as well as to reduce measurement time and costs. This study investigated the representative elementary length (REL) of sandy and clayey soils for µ(es) measurements. Two radioactive sources were employed ((241)Am and (137)Cs), three collimators (2-4 mm diameters), and 14 thickness (x) samples (2-15 cm). Results indicated ideal thickness intervals of 12-15 and 2-4 cm for the sources (137)Cs and (241)Am, respectively. The application of such results in representative elementary area (REA) evaluations in clayey soil clods via CT indicated that µ(es) average values obtained for x > 4 cm and source (241)Am might induce to the use of samples which are not large enough for soil bulk density evaluations (ρ(s)). As a consequence, ρ(s) might be under- or overestimated, generating inaccurate conclusions about the physical quality of the soil under study.

Perfil de sensibilidade de células sésseis e planctônicas de Escherichia coli a antimicrobianos usados no tratamento da mastite bovina / Sensitivity profile of planktonic and sessile cells of Escherichia coli to antimicrobial used in the treatment of bovine mastitis

Escherichia coli é um micro-organismo altamente adaptativo e sua habilidade em formar biofilmes pode ser fundamental na resistência a tratamentos com antimicrobianos. A avaliação da concentração mínima inibitória (CMI) vem sendo utilizada para verificar a sensibilidade dos micro-organismos aos antimicrobianos. Entretanto, quando se avaliam células sésseis, a concentração do antimicrobiano requerido para erradicação do biofilme é maior do que a determinada pela CMI. Objetivou-se comparar as CMI com as concentrações mínimas de erradicação de biofilmes (CMEB) de antimicrobianos usados no tratamento da mastite em 27 isolados de E. coli produtores de biofilmes provenientes de mastite. Os isolados foram submetidos a testes de sensibilidade a antimicrobianos usados no tratamento da mastite, tanto para células planctônicas, por meio da CMI, quanto para células sésseis, pela avaliação da CMEB. Os resultados revelaram uma alta sensibilidade: apenas quatro (14,8%) isolados obtiveram valores da CMI elevados, variando de 4 a 10µg/mL, sendo classificados como resistentes. Para os demais isolados (85,2%), os valores foram menores, variando de 0,125 a 2µg/mL, classificados como sensíveis. A avaliação de CMEB indicou que a concentração dos antimicrobianos necessária para eliminar as células sésseis variou de 100µg/mL a 500µg/mL. Os valores de CMEB foram significativamente maiores nos isolados grandes e moderados produtores de biofilmes em relação aos isolados fracos produtores de biofilmes (p<0,001). Não houve correlação entre os valores de CMEB e CMI (p>0,05). A escolha da terapêutica antimicrobiana correta para o tratamento de infecções intramamárias em bovinos relacionadas com a produção de biofilmes parece exigir a aplicação de testes mais específicos. Testes de susceptibilidade antimicrobiana baseados apenas em valores de CMI mostraram-se ineficazes em determinar com precisão a susceptibilidade das células bacterianas sésseis.

Escherichia coli is a highly adaptive microorganism. Its ability to form biofilms may be critical for resistance to antimicrobial treatments. Evaluation of minimum inhibitory concentration (MIC) has been used to check the sensitivity of microorganisms to antibiotics, however, when evaluating sessile cells, the required antibiotic concentration to eradicate biofilm is greater than determined by MIC. This study aimed to compare MIC with minimum biofilm eradication concentration (MBEC) of antimicrobials used in mastitis treatment in 27 E. coli biofilm producers isolates from mastitis. Isolates were tested for sensitivity to antimicrobials used in mastitis treatment, for both planktonic cells (by CMI) and sessile cells (by MBEC). The results revealed high sensitivity: only four (14.8%) isolates showed high MIC values, ranging from 4 to 10g/mL and they were classified as resistant. All other isolates (85.2%) showed lower values, ranging from 0.125 to 2mg/mL, and they were classified as sensitive. Evaluation of MBEC indicated that concentration of antimicrobial needed to remove sessile cells ranged from 100mg/mL to 500mg/mL. MBEC values were significantly higher in large and moderate biofilm producers isolates regarding weak biofilm producers isolates (p<0.001). There was no correlation between MBEC and CMI values (p>0.05). The correct choice of antimicrobial therapy for treatment of mammary infections in cattle related to biofilm production seems to require application of more specific tests. Antimicrobial susceptibility testing based only on MIC values proved ineffectiveness to accurately determination the susceptibility of sessile bacterial cells.