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Recent advances in AI and intelligent vehicle technology hold the promise of revolutionizing mobility and transportation through advanced driver assistance systems (ADAS). Certain cognitive factors, such as impulsivity and inhibitory control have been shown to relate to risky driving behavior and on-road risk-taking. However, existing systems fail to leverage such factors in assistive driving technologies adequately. Varying the levels of these cognitive factors could influence the effectiveness and acceptance of ADAS interfaces. We demonstrate an approach for personalizing driver interaction via driver safety interfaces that are are triggered based on the inference of the driver's latent cognitive states from their driving behavior. To accomplish this, we adopt a data-driven approach and train a recurrent neural network to infer impulsivity and inhibitory control from recent driving behavior. The network is trained on a population of human drivers to infer impulsivity and inhibitory control from recent driving behavior. Using data collected from a high-fidelity vehicle motion simulator experiment, we demonstrate the ability to deduce these factors from driver behavior. We then use these inferred factors to determine instantly whether or not to engage a driver safety interface. This approach was evaluated using leave-one-out cross validation using actual human data. Our evaluations reveal that our personalized driver safety interface that captures the cognitive profile of the driver is more effective in influencing driver behavior in yellow light zones by reducing their inclination to run through them.
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Condução de Veículo , Cognição , Humanos , Condução de Veículo/psicologia , Cognição/fisiologia , Masculino , Segurança , Feminino , Adulto , Assunção de Riscos , Comportamento Impulsivo , Redes Neurais de Computação , Simulação por Computador , Acidentes de Trânsito/prevenção & controle , Acidentes de Trânsito/psicologiaRESUMO
Advances in autonomous driving provide an opportunity for AI-assisted driving instruction that directly addresses the critical need for human driving improvement. How should an AI instructor convey information to promote learning? In a pre-post experiment (n = 41), we tested the impact of an AI Coach's explanatory communications modeled after performance driving expert instructions. Participants were divided into four (4) groups to assess two (2) dimensions of the AI coach's explanations: information type ('what' and 'why'-type explanations) and presentation modality (auditory and visual). We compare how different explanatory techniques impact driving performance, cognitive load, confidence, expertise, and trust via observational learning. Through interview, we delineate participant learning processes. Results show AI coaching can effectively teach performance driving skills to novices. We find the type and modality of information influences performance outcomes. Differences in how successfully participants learned are attributed to how information directs attention, mitigates uncertainty, and influences overload experienced by participants. Results suggest efficient, modality-appropriate explanations should be opted for when designing effective HMI communications that can instruct without overwhelming. Further, results support the need to align communications with human learning and cognitive processes. We provide eight design implications for future autonomous vehicle HMI and AI coach design.
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Condução de Veículo , Cognição , Confiança , Humanos , Condução de Veículo/psicologia , Masculino , Feminino , Cognição/fisiologia , Adulto , Confiança/psicologia , Inteligência Artificial , Adulto Jovem , Aprendizagem/fisiologiaRESUMO
OBJECTIVE: To evaluate the performance of prenatal screening for common autosomal trisomies in twin pregnancies through the use of rolling-circle replication (RCR)-cfDNA as a first-tier test. METHOD: Prospective multicenter study. Women who underwent prenatal screening for trisomy (T) 21, 18 and 13 between January 2019 and March 2022 in twin pregnancies were included. Patients were included in two centers. The primary endpoint was the rate of no-call results in women who received prenatal screening for common autosomal trisomies by RCR-cfDNA at the first attempt, compared to that in prospectively collected samples from 16,382 singleton pregnancies. The secondary endpoints were the performance indices of the RCR-cfDNA. RESULTS: 862 twin pregnancies underwent screening for T21, T18 and T13 by RCR-cfDNA testing at 10-33 weeks' gestation. The RCR-cfDNA tests provided a no-call result from the first sample obtained from the patients in 107 (0.7%) singleton and 17 (2.0%) twin pregnancies. Multivariable regression analysis demonstrated that significant independent predictors of test failure were twin pregnancy and in vitro fertilization conception. All cases of T21 (n = 20/862; 2.3%), T18 (n = 4/862; 0.5%) and T13 (n = 1/862; 0.1%) were correctly detected by RCR-cfDNA (respectively, 20, 4 and 1 cases). Sensitivity was 100% (95% CI, 83.1%-100%), 100% (95% CI 39.8%-100%) and 100% (95% CI 2.5%-100%) for T21, T18 and T13, respectively, in twin pregnancies. CONCLUSION: The RCR-cfDNA test appears to have good accuracy with a low rate of no-call results in a cohort of twin pregnancies for the detection of the most frequent autosomal trisomies.
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Ácidos Nucleicos Livres , Gravidez de Gêmeos , Humanos , Feminino , Gravidez , Gravidez de Gêmeos/sangue , Gravidez de Gêmeos/genética , Adulto , Estudos Prospectivos , Ácidos Nucleicos Livres/análise , Ácidos Nucleicos Livres/sangue , Trissomia/diagnóstico , Trissomia/genéticaRESUMO
The yeast-encapsulated orange oil (YEOO) is a novel larvicide under development against vector mosquitoes. Despite its efficiency against Aedes aegypti (L.) in small scale experiments, its applicability in vector control can be influenced by other effects on mosquito behaviour or physiology. For this reason, the impact of YEOO particles in mosquito oviposition was evaluated in laboratory and semi-field conditions. Oviposition assays with one gravid Aedes aegypti female were carried under laboratory and semi-field conditions with natural light and temperature fluctuation. For all ovitraps, the number of eggs was manually counted in the wooden paddle and in the solution of each ovitrap. The proportion of eggs between substrates (wooden paddle and solution) varied between conditions, with females in laboratory presenting a lower preference to lay eggs in paddles when compared with studies in semi-field. This behaviour shifts in laboratory can create challenges to extrapolate results from laboratory to the field. Here, studies in both conditions indicate a similar impact of YEOO particles in Aedes aegypti oviposition. The potential treatment concentration of YEOO particles presents a strong repellent/deterrent effect (-0.559 > OAI > -0.760) within the initial 72h of application when compared with water, and weak repellent/deterrent signal (OAI = -0.220) when compared against inactivated yeast. Control ovitraps with water were more positive for egg presence than treated ovitraps, while ovitraps with YEOO particles and inactivated yeast present similar number of positive ovitraps. It is possible that the repellent/deterrent action is partially driven by the delivery system, since most times Citrus sinensis EO oviposition repellent/deterrent signal is weak, and it seem influenced by solvent/delivery used. However, it is unclear how the yeast wall that protect/surrounds the orange oil will negatively affect oviposition since live yeast are normally consider an attractant for mosquito oviposition.
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Aedes , Controle de Mosquitos , Oviposição , Óleos de Plantas , Aedes/fisiologia , Aedes/efeitos dos fármacos , Animais , Oviposição/efeitos dos fármacos , Feminino , Óleos de Plantas/farmacologia , Controle de Mosquitos/métodos , Mosquitos Vetores/fisiologia , Mosquitos Vetores/efeitos dos fármacos , Saccharomyces cerevisiae/fisiologia , Repelentes de Insetos/farmacologiaRESUMO
Coprological and serological diagnostic tests were compared to define the status of a pig farm with regard to Ascaris suum. On each of the 100 farms in France visited for the study, 10 blood samples were taken from pigs at the end of fattening (at least 22 weeks old) and 20 to 30 faecal samples were taken, depending on the category of animals present on the farm (10 sows, 10 piglets aged 10 to 12 weeks and 10 pigs at the end of fattening, aged at least 22 weeks). A SERASCA® ELISA test (Laboratory of Parasitology, Ghent University) was performed on each blood sample (cut-off 0.5) and a coprological analysis on each faecal sample. A Bayesian approach was used to estimate the sensitivity and specificity of the coprological and serological tests. A farm was considered positive if at least one A. suum egg was observed in the faecal samples. With regard to the serological test, various hypotheses were tested in order to define the number of seropositive animals required to consider a farm positive for A. suum. The coprological test has very good specificity in the search for A. suum, whether 20 or 30 samples are taken per farm. However, even with an increase in the number of samples, the sensitivity of this diagnostic approach is very low (less than 30%). On the other hand, the serological diagnostic method, which consists of taking blood samples from 10 animals at the end of fattening, has good sensitivity and seems better suited to defining the status of a farm with regard to A. suum, provided that a farm is considered seropositive only if two out of 10 samples are positive.
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Ascaríase , Ascaris suum , Teorema de Bayes , Fezes , Sensibilidade e Especificidade , Doenças dos Suínos , Animais , Ascaris suum/isolamento & purificação , Ascaris suum/imunologia , Doenças dos Suínos/diagnóstico , Doenças dos Suínos/parasitologia , Fezes/parasitologia , Fezes/química , Suínos , Ascaríase/diagnóstico , Ascaríase/veterinária , Ascaríase/parasitologia , Testes Sorológicos/veterinária , Testes Sorológicos/métodos , Ensaio de Imunoadsorção Enzimática/veterinária , Ensaio de Imunoadsorção Enzimática/métodos , França , Fazendas , Anticorpos Anti-Helmínticos/sangueRESUMO
Beekeeping directly depends on the floral biodiversity available to honey bees. In tropical regions, where nectar and pollen resources are numerous, the botanical origin of some honey is still under discussion. A precise knowledge of plants foraged by honey bees is useful to understand and certify the botanical origin of honey. In this study, attention was paid to honey samples from the French Guiana Atlantic coast where beekeepers generally place their hives in four types of biotopes: seaside vegetation, mangrove, savannah, and secondary forest. Pollen analysis of 87 honey samples enabled the identification of major plants visited by Africanized honey bees during the dry season (approximately from July to January). Through melissopalynologic analysis, 51 pollen types were identified and classified according to their relative presence. Frequently observed pollens (with relative presence > 50%) in French Guiana kinds of honey were those from Mimosa pudica, Cocos sp., Rhyncospora sp., Avicennia germinans, Paspalum sp., Spermacoce verticillata, Tapirira guianensis, Cecropia sp., Myrtaceae sp., Mauritia flexuosa sp., Solanum sp., and Protium sp. In many honeys, only M. pudica was over-represented (relative frequency > 90%). Color and electrical conductivity in French Guiana honeys exhibit significant variations, with color ranging from 27 mm to 110 mm Pfund, and electrical conductivity ranging from 0.35 to 1.22 mS/cm.
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OBJECTIVE: In singleton pregnancies, the use of cell-free DNA (cfDNA) analysis as a screening test for common fetal trisomies has spread worldwide though we still lack sufficient data for its use in triplet pregnancies. The objective of this study is to assess the performance of cfDNA testing in detecting fetal aneuploidies in triplet pregnancies as a first-tier test. METHOD: We performed a retrospective cohort study including data from pregnant women with a triplet pregnancy who underwent cfDNA testing between May 1, 2017, and January 15, 2020. cfDNA was obtained by massive parallel sequencing (VeriSeq NIPT solution; Illumina®). The objectives of the study were to assess the diagnostic performance of cfDNA testing for trisomy 21 (T21) (primary outcome), trisomy 18 (T18) and 13 (secondary outcomes). RESULTS: During the study period, cfDNA testing was performed in 255 women with triplet pregnancy, of which 165 (64.7%) had a neonatal outcome available. Three tests were positive for T21, one of which was confirmed by an antenatal karyotype, and the other was confirmed at birth. The third case did not undergo an invasive procedure and was not confirmed at birth (false positive). In one case, cfDNA testing was positive for T18 and was confirmed by an antenatal karyotype. There were no cases of trisomy 13 in the cohort. The no-call rate was 2.4% at first sampling. Fifty-eight (22.7%) women had embryo reduction, which in 40 (69%) of whom was performed after the cfDNA test result. CONCLUSION: cfDNA testing could be offered as primary screening for main fetal aneuploidies in triplet pregnancies after provision of appropriate patient information.
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Ácidos Nucleicos Livres , Gravidez de Trigêmeos , Humanos , Feminino , Gravidez , Estudos Retrospectivos , Ácidos Nucleicos Livres/sangue , Ácidos Nucleicos Livres/análise , Adulto , Síndrome da Trissomía do Cromossomo 18/diagnóstico , Síndrome da Trissomía do Cromossomo 18/genética , Síndrome da Trissomía do Cromossomo 18/sangue , Trissomia/diagnóstico , Trissomia/genética , Teste Pré-Natal não Invasivo/métodos , Teste Pré-Natal não Invasivo/estatística & dados numéricos , Teste Pré-Natal não Invasivo/normas , Síndrome da Trissomia do Cromossomo 13/diagnóstico , Síndrome da Trissomia do Cromossomo 13/sangue , Síndrome da Trissomia do Cromossomo 13/genética , Estudos de Coortes , Síndrome de Down/diagnóstico , Síndrome de Down/genética , Testes para Triagem do Soro Materno/métodos , Testes para Triagem do Soro Materno/estatística & dados numéricos , Diagnóstico Pré-Natal/métodos , Diagnóstico Pré-Natal/normasRESUMO
The study aimed to investigate the chemical composition and antimicrobial and antioxidant activities of the essential oil from dried seeds of Xylopia aethiopica. The essential oil was obtained by hydrodistillation and analyzed by GC/FID and GC/MS. The essential oil yield was 1.35%. Forty-nine compounds were identified in the essential oil with 1,8-cineole (16.3%), ß-pinene (14.8%), trans-pinocarveol (9.1%), myrtenol (8.3%), α-pinene (5.9%), and terpinen-4-ol (5.6%) as major components. The antimicrobial activity of this essential oil was studied using disk diffusion and broth microdilution methods on four bacteria (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa) and one fungus (Candida albicans). The essential oil exhibited excellent activity against S. aureus, E. faecalis, and C. albicans and moderate activity against E. coli. Among all strains tested, C. albicans showed the best sensitivity with a MIC of 50 mg/mL. The antioxidant activity was examined using a DPPH-free radical scavenging assay. The essential oil of X. aethiopica showed low antioxidant activity (IC50 = 784.604 ± 0.320 mg/mL) compared to that of ascorbic acid and the reference compound (IC50 = 0.163 ± 0.003 mg/mL). The results indicate that consumption of X. aethiopica seeds can reduce the virulence of food-borne pathogens and their resistance to antibiotics.
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(1) Background: Aspergillus flavus is a cosmopolitan mold with medical, veterinary, and agronomic concerns. Its morphological similarity to other cryptic species of the Flavi section requires molecular identification techniques that are not routinely performed. For clinical isolates of Aspergillus section Flavi, we present the molecular identification, susceptibility to six antifungal agents, and clinical context of source patients. (2) Methods: One hundred forty fungal clinical isolates were included in the study. These isolates, recovered over a 15-year period (2001-2015), were identified based on their morphological characteristics as belonging to section Flavi. After the subculture, sequencing of a part of the ß-tubulin and calmodulin genes was performed, and resistance to azole antifungals was screened on agar plates containing itraconazole and voriconazole. Minimum inhibitory concentrations were determined for 120 isolates by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) broth microdilution method. (3) Results: Partial ß-tubulin and calmodulin sequences analysis showed that 138/140 isolates were A. flavus sensu stricto, 1 isolate was A. parasiticus/sojae, and 1 was A. nomiae. Many of the isolates came from samples collected in the context of respiratory tract colonization. Among probable or proven aspergillosis, respiratory infections were the most frequent, followed by ENT infections. Antifungal susceptibility testing was available for isolates (n = 120, all A. flavus ss) from one hospital. The MIC range (geometric mean MIC) in mg/L was 0.5-8 (0.77), 0.5-8 (1.03), 0.125-2 (0.25), 0.03-2 (0.22), 0.25-8 (1.91), and 0.03-0.125 (0.061) for voriconazole, isavuconazole, itraconazole, posaconazole, amphotericin B, and caspofungin, respectively. Two (1.67%) isolates showed resistance to isavuconazole according to current EUCAST breakpoints with MICs at 8 mg/L for isavuconazole and voriconazole. One of these two isolates was also resistant to itraconazole with MIC at 2 mg/L. (4) Conclusions: The present characterization of a large collection of Aspergillus belonging to the Flavi section confirmed that A. flavus ss is the predominant species. It is mainly implicated in respiratory and ENT infections. The emergence of resistance highlights the need to perform susceptibility tests on section Flavi isolates.
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Various honeys from French Guiana were collected and analyzed to investigate their volatile fraction composition and antioxidant activity. Volatile composition was assessed using HS-SPME/GC, GC-MS technique. Oxygenated monoterpenes like hotrienol (0.5-45.3%) were found as major molecules, followed by non terpenic compounds like phenylacetaldehyde (0.8-18.2%) or 3-hydroxy-4-phenyl-2-butanone (0.1-29.3%). Three chemical groups using statistical analysis were classified within investigated honey samples: norisoprenoids/shikimates, mevalonate and their combination. Total phenolics content (TPC) was determined by Folin-Ciocalteu method. Antioxidant activity was assessed by oxygen radical absorbance capacity (ORAC) and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays. TPC and anti-radical activity were compared with multifloral honeys from neighboring regions, indicating the possible presence of compounds from the polyphenol family. These results are promising for further biological studies involving honeys from French Guiana.
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Essential oils from wild Corsican hops have never been described before. Following selective harvesting and extraction of plant material, chemical analysis was performed by GC-FID and GC-MS. Subsequent quantitative analysis demonstrated significant inter-stations variability due to pedoclimatic conditions. These singularities produce organoleptic differences, especially within Italian hops, which are the current benchmark for the Mediterranean hops' population. Corsican wild hops are no exception. Accordingly, three olfactive bouquets were identified by a panel of selected and trained sensory analysts: woody herbaceous ginger notes, herbaceous citrus notes, and common notes. These bouquets appeared to be correlated to pedoclimatic parameters mentioned earlier such as altitude and proximity to the sea. A very rare and appreciated bouquet was associated with high levels of zingiberene in hops growing at moderate altitude and relatively far from the coastline. This study shows the importance of growing sites and pedoclimatic conditions to produce hops with the desired organoleptic notes during the beer making process and provides detailed identification of essential oils from Corsican wild hops.
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Neste ensaio revisitamos uma problemática básica presente na confluência entre as sociologias do lazer e do trabalho, isto é, de que o tempo livre estaria acorrentado ao tempo de trabalho. Lembramos que são conhecidas as distinções conceituais sobre as atividades liberadas dos tempos do trabalho e as atividades exercidas por prazer no tempo livre. Assim, neste escrito partirmos da premissa crítica de que no atual capitalismo global não podemos isolar as esferas do tempo social cotidiano do tempo social do trabalho. Destacamos a transição do tempo livre de caráter fordista para o tempo livre de caráter toyotista, cuja face neoliberal assumiu uma forma extremamente perversa, conjugando uma agenda sistemática de privatização das instâncias de socialização das tensões e contingências da vida coletiva, de flexibilização dos constrangimentos, de exploração do Trabalho pelo Capital e de desregulação generalizada do Mercado. Direitos Trabalhistas perdidos, desemprego estrutural, instabilidade biográfica e consumismo narcísico parelham a trajetória das massas atomizadas de trabalhadores multi-tarefeiros e proativos, eternamente ocupados e envolvidos em modo full-time. Concluímos, pois, que o tempo livre, sempre inscrito em coerção ideológica, agora é destroçado perante uma sociedade da performance e do cansaço, que não dorme e que está eternamente ocupada.
In this essay we revisit and discuss a basic problem at the confluence between the sociologies of leisure and the sociologies of work, that is, that the free time would be chained to the work time. We recall that are known the conceptual distinctions about the activities released from the time of work and the activities exercised for pleasure in the free time. Thus, in this writing we start from the critical premise that in the current global capitalism we cannot isolate the spheres of everyday social time from the social time of work. We highlight the transition from free time of a Fordist character to free time of a Toyotist character, whose neoliberal face has assumed an extremely perverse form, combining a systematic agenda of privatization of the instances of socialization of the tensions and contingencies of collective life, of flexibilization of the constraints to the exploitation of Labor by Capital and of generalized deregulation of the Market. Lost labor rights, structural unemployment, biographical instability, and narcissistic consumerism pair the trajectory of the atomized masses of multi-tasking, proactive workers, eternally occupied and engaged in full-time mode. We conclude, therefore, that free time, always inscribed in ideological coercion, is now shattered before a society of performance and fatigue, which does not sleep and is eternally occupied.
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Equilíbrio Trabalho-Vida , Atividades de LazerRESUMO
The grapevine is vulnerable to diseases, deficiencies, and pests, leading to significant yield losses. Current disease controls involve monitoring and spraying phytosanitary products at the vineyard block scale. However, automatic detection of disease symptoms could reduce the use of these products and treat diseases before they spread. Flavescence dorée (FD), a highly infectious disease that causes significant yield losses, is only diagnosed by identifying symptoms on three grapevine organs: leaf, shoot, and bunch. Its diagnosis is carried out by scouting experts, as many other diseases and stresses, either biotic or abiotic, imply similar symptoms (but not all at the same time). These experts need a decision support tool to improve their scouting efficiency. To address this, a dataset of 1483 RGB images of grapevines affected by various diseases and stresses, including FD, was acquired by proximal sensing. The images were taken in the field at a distance of 1-2 meters to capture entire grapevines and an industrial flash was ensuring a constant luminance on the images regardless of the environmental circumstances. Images of 5 grape varieties (Cabernet sauvignon, Cabernet franc, Merlot, Ugni blanc and Sauvignon blanc) were acquired during 2 years (2020 and 2021). Two types of annotations were made: expert diagnosis at the grapevine scale in the field and symptom annotations at the leaf, shoot, and bunch levels on computer. On 744 images, the leaves were annotated and divided into three classes: 'FD symptomatic leaves', 'Esca symptomatic leaves', and 'Confounding leaves'. Symptomatic bunches and shoots were, in addition of leaves, annotated on 110 images using bounding boxes and broken lines, respectively. Additionally, 128 segmentation masks were created to allow the detection of the symptomatic shoots and bunches by segmentation algorithms and compare the results to those of the detection algorithms.
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BACKGROUND AND OBJECTIVE: GLUT1 deficiency syndrome (Glut1DS) is a treatable neurometabolic disease that causes a wide range of neurologic symptoms in children and adults. However, its diagnosis relies on an invasive test, that is, a lumbar puncture (LP) to measure glycorrhachia, and sometimes complex molecular analyses of the SLC2A1 gene. This procedure limits the number of patients able to receive the standard of care. We wished to validate the diagnostic performance of METAglut1, a simple blood test that quantifies GLUT1 on the erythrocyte surface. METHODS: We performed a multicenter validation study in France, involving 33 centers. We studied 2 patient cohorts: a prospective cohort consisting of patients with a clinical suspicion of Glut1DS explored through the reference strategy, that is, LP and analyses of the SLC2A1 gene, and a retrospective cohort that included patients previously diagnosed with Glut1DS. All patients were blind-tested with METAglut1. RESULTS: We analyzed 428 patients in the prospective cohort, including 15 patients newly diagnosed with Glut1DS, and 67 patients in the retrospective cohort. METAglut1 was 80% sensitive and >99% specific for the diagnosis of Glut1DS. Concordance analyses showed a substantial agreement between METAglut1 and glycorrhachia. In the prospective cohort, the positive predictive value of METAglut1 was slightly higher than that of glycorrhachia. METAglut1 succeeded to identify patients with Glut1DS with SCL2A1 mosaicism and variants of unknown significance. DISCUSSION: METAglut1 is an easily performed, robust, and noninvasive diagnostic test for the diagnosis of Glut1DS, which allows wide screening of children and adults, including those with atypical forms of this treatable condition. CLASSIFICATION OF EVIDENCE: This study provides Class I evidence that a positive METAglut1 test accurately distinguishes patients with suspected GLUT1 deficiency syndrome from other neurologic syndromes as compared with invasive and genetic testing.
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Erros Inatos do Metabolismo dos Carboidratos , Adulto , Criança , Humanos , Estudos Retrospectivos , Estudos Prospectivos , Erros Inatos do Metabolismo dos Carboidratos/diagnóstico , Erros Inatos do Metabolismo dos Carboidratos/genética , Proteínas de Transporte de Monossacarídeos/genéticaRESUMO
Anadenanthera colubrina, popularly known as white angico, is a species extensively cultivated in Brazil, mainly in the cerrado region, including the state of Piauí. This study examines the development of films composed of white angico gum (WAG) and chitosan (CHI) and containing chlorhexidine (CHX), an antimicrobial agent. The solvent casting method was used to prepare films. Different combinations and concentrations of WAG and CHI were used to obtain films with good physicochemical characteristics. Properties such as the in vitro swelling ratio, the disintegration time, folding endurance, and the drug content were determined. The selected formulations were characterised by scanning electron microscopy, Fourier-transform infrared spectroscopy, differential scanning calorimetry, thermogravimetric analysis, and X-ray diffraction, and the CHX release time and antimicrobial activity were evaluated. CHX showed a homogenous distribution in all CHI/WAG film formulations. The optimised films showed good physicochemical properties with 80% CHX release over 26 h, which is considered promising for local treatment of severe lesions in the mouth. Cytotoxicity tests of the films did not show toxicity. The antimicrobial and antifungal effects were very effective against the tested microorganisms.
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Anti-Infecciosos , Quitosana , Clorexidina/farmacologia , Clorexidina/química , Quitosana/química , Anti-Infecciosos/farmacologia , Antifúngicos , Brasil , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios XRESUMO
In this study, the growth of six L. monocytogenes strains isolated from different fish products was quantified and modeled in smoked salmon pâté at a temperature ranging from 2 to 20 °C. The experimental data obtained for each strain was fitted to the primary growth model of Baranyi and Roberts to estimate the following kinetic parameters: lag phase (λ), maximum specific growth rate (µmax), and maximum cell density (Nmax). Then, the effect of storage temperature on the obtained µmax values was modeled by the Ratkowsky secondary model. In general, the six L. monocytogenes strains showed rapid growth in salmon pâté at all storage temperatures, with a relatively short lag phase λ, even at 2 °C. The growth behavior among the tested strains was similar at the same storage temperature, although significant differences were found for the parameters λ and µmax. Besides, the growth variations among the strains did not follow a regular pattern. The estimated secondary model parameter Tmin ranged from -4.25 to -3.19 °C. This study provides accurate predictive models for the growth of L. monocytogenes in fish pâtés that can be used in shelf life and microbial risk assessment studies. In addition, the models generated in this work can be implemented in predictive modeling tools and repositories that can be reliably and easily used by the fish industry and end-users to establish measures aimed at controlling the growth of L. monocytogenes in fish-based pâtés.
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The potential of image proximal sensing for agricultural applications has been a prolific scientific subject in the recent literature. Its main appeal lies in the sensing of precise information about plant status, which is either harder or impossible to extract from lower-resolution downward-looking image sensors such as satellite or drone imagery. Yet, many theoretical and practical problems arise when dealing with proximal sensing, especially on perennial crops such as vineyards. Indeed, vineyards exhibit challenging physical obstacles and many degrees of variability in their layout. In this paper, we present the design of a mobile camera suited to vineyards and harsh experimental conditions, as well as the results and assessments of 8 years' worth of studies using that camera. These projects ranged from in-field yield estimation (berry counting) to disease detection, providing new insights on typical viticulture problems that could also be generalized to orchard crops. Different recommendations are then provided using small case studies, such as the difficulties related to framing plots with different structures or the mounting of the sensor on a moving vehicle. While results stress the obvious importance and strong benefits of a thorough experimental design, they also indicate some inescapable pitfalls, illustrating the need for more robust image analysis algorithms and better databases. We believe sharing that experience with the scientific community can only benefit the future development of these innovative approaches.
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Agricultura , Algoritmos , Fazendas , Retroalimentação , Agricultura/métodos , Processamento de Imagem Assistida por Computador , Produtos AgrícolasRESUMO
Volatiles metabolites from the liverwort Plagiochila porelloides harvested in Corsica were investigated by chromatographic and spectroscopic methods. In addition to already reported constituents, three new compounds were isolated by preparative chromatography and their structures were elucidated by mass spectrometry (MS) and NMR experiments. Hence, an atypic aliphatic compound, named 1,2-dihydro-4,5-dehydronerolidol and two isomers, (E) and (Z), possessing an unusual humbertiane skeleton (called p-menth-1-en-3-[2-methylbut-1-enyl]-8-ol) are newly reported and fully characterized in this work. The in vitro antiprotozoal activity of essential oil and extract of P. porelloides against Trypanosoma brucei brucei and Leishmania mexicana mexicana and cytotoxicity were determined. Essential oil and Et2O extract showed a moderate activity against T. brucei with IC50 values: 2.03 and 5.18 µg/mL, respectively. It is noteworthy that only the essential oil showed a high selectivity (SI = 11.7). Diethyl oxide extract exhibited moderate anticancer (cancerous macrophage-like murine cells) activity and also cytotoxicity (human normal fibroblast) with IC50 values: 1.25 and 2.96 µg/mL, respectively.
Assuntos
Antiprotozoários , Hepatófitas , Óleos Voláteis , Trypanosoma brucei brucei , Animais , Camundongos , Humanos , Óleos Voláteis/farmacologia , Óleos Voláteis/química , Antiprotozoários/farmacologia , Antiprotozoários/química , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Plasmodium falciparumRESUMO
In predictive microbiology, primary and secondary models can be used to predict microbial growth, usually in a two-step modelling approach. The inverse dynamic modelling approach is an alternative method to direct modelling methods, in which the primary and secondary models are fitted simultaneously from non-isothermal data, minimising experimental effort and costs. Thus, the main aim of the present study was to compare the prediction capabilities of the mathematical modelling approaches used for calculating growth kinetics of microorganisms in predictive food microbiology field. For this purpose, the bacterial growth data of Pseudomonas spp. in oyster mushroom (Pleurotus ostreatus) subjected to isothermal and non-isothermal storage temperatures were collected from previously published growth curves. Temperature-dependent kinetic growth parameters (maximum specific growth rate 'µmax' and lag phase duration 'λ') were described as a function of storage temperature using the direct two-step, direct one-step and inverse dynamic modelling approach based on Baranyi and Huang models. The fitting capability of the modelling approaches was separately compared, and the one-step modelling approach for the direct methods provided better goodness of fit results regardless of used primary models, which leads the Huang model with being RMSE = 0.226 and R2adj = 0.949 became best for direct methods. Like seen in direct methods, the Huang model gave better goodness of fit results than Baranyi model for inverse method. Results revealed there was no significant difference (p > 0.05) between the growth kinetic parameters obtained from direct one-step modelling approach and inverse modelling approaches based on the Huang model. Satisfactorily statistical indexes show that the inverse dynamic modelling approach can be reliably used as an alternative way of describing the growth behaviour of Pseudomonas spp. in oyster mushroom in a fast and minimum labour effort.
Assuntos
Pleurotus , Contagem de Colônia Microbiana , Microbiologia de Alimentos , Cinética , Modelos Biológicos , Pseudomonas , TemperaturaRESUMO
About 0.3% of all variants are due to de novo mobile element insertions (MEIs). The massive development of next-generation sequencing has made it possible to identify MEIs on a large scale. We analyzed exome sequencing (ES) data from 3232 individuals (2410 probands) with developmental and/or neurological abnormalities, with MELT, a tool designed to identify MEIs. The results were filtered by frequency, impacted region and gene function. Following phenotype comparison, two candidates were identified in two unrelated probands. The first mobile element (ME) was found in a patient referred for poikilodermia. A homozygous insertion was identified in the FERMT1 gene involved in Kindler syndrome. RNA study confirmed its pathological impact on splicing. The second ME was a de novo Alu insertion in the GRIN2B gene involved in intellectual disability, and detected in a patient with a developmental disorder. The frequency of de novo exonic MEIs in our study is concordant with previous studies on ES data. This project, which aimed to identify pathological MEIs in the coding sequence of genes, confirms that including detection of MEs in the ES pipeline can increase the diagnostic rate. This work provides additional evidence that ES could be used alone as a diagnostic exam.