Antígeno leucocitario humano-G soluble en el lavado broncoalveolar de pacientes con cáncer pulmonar / Soluble human leukocyte antigen-G in the bronchoalveolar lavage of lung cancer patients

Antecedentes. La molécula antígeno leucocitario humano G (HLA-G), en sus formas unida a membrana y soluble, tiene como función principal inhibir la respuesta inmune actuando sobre los linfocitos T/CD4+, T/citotóxicos, células NK y células dendríticas. El cáncer de pulmón es una de las principales causas de muerte en el mundo, con una alta tasa de incidencia anual tanto en mujeres como en hombres. Algunos estudios han reportado un incremento de HLA-G sérica en el cáncer de pulmón, probablemente como un mecanismo de escape de la célula tumoral a la respuesta inmune antitumoral. En este estudio se midió la concentración de HLA-G soluble, en el lavado broncoalveolar (LBA), en pacientes con cáncer de pulmón primario y metastásico para determinar su relación con el tipo histológico tumoral y estado general del paciente usando la escala de Karnofsky. Métodos. Se incluyeron 31 pacientes con diagnóstico de cáncer de pulmón y mediante fibrobroncoscopia se tomó biopsia de la neoplasia para determinar el tipo de tumor usando la coloración de hematoxilina y eosina, y líquido del lavado broncoalveolar para medir la concentración de la molécula HLA-G soluble mediante un ELISA tipo sándwich. Resultados. El valor medio de la HLA-G soluble fue de 49,04ng/ml. No se observó ninguna correlación entre los niveles de HLA-G soluble y la edad, género o índice de tabaquismo. Se observó una diferencia altamente significativa en los niveles de HLA-G soluble en LBA de pacientes con diversos tipos histológicos de cáncer de pulmón, principalmente en tumores metastásicos. El índice de Karnofsky mostró una correlación significativa e inversa con el nivel de HLA-G soluble en LBA. Conclusiones. La proteína HLA-G soluble puede ser útil como marcador pronóstico del cáncer pulmonar al asociarse significativamente a los tumores metastásicos y a los pacientes con menor índice de Karnofsky

Background. The main function of the HLA-G molecule in its membrane-bound and soluble forms is to inhibit the immune response by acting on CD4+ T cells, cytotoxic T cells, NK cells and dendritic cells. Lung cancer is a leading cause of death worldwide, and annual incidence is high in both women and men. Some studies have reported an increase of HLA-G serum levels in lung cancer, probably generated by tumor cells escaping the antitumor immune response. In this study the concentration of soluble HLA-G in bronchoalveolar lavage (BAL) in patients with primary and metastatic lung cancer was measured to determine its relation with tumor histological type and overall patient status according to the Karnofsky scale. Methods. Thirty-one lung cancer patients were included. A tumor biopsy was obtained by bronchoscopy and the tumor type was determined by hematoxylin and eosin staining. BAL samples were obtained to measure soluble HLA-G concentrations in an ELISA sandwich assay. Results. The average value of soluble HLA-G was 49.04ng/mL. No correlation between soluble HLA-G levels and age, gender or smoking was observed. A highly significant difference was observed in the levels of soluble HLA-G in BAL from patients with different histological types of lung cancer, especially in metastatic tumors. The Karnofsky index showed a significant and inverse correlation with soluble HLA-G levels in BAL. Conclusions. Soluble HLA-G protein is significantly associated with metastatic tumors and patients with lower Karnofsky index and may be useful as a prognostic marker in lung cancer

Soluble Human Leukocyte Antigen-G in the Bronchoalveolar Lavage of Lung Cancer Patients. / Antígeno leucocitario humano-G soluble en el lavado broncoalveolar de pacientes con cáncer pulmonar.

BACKGROUND: The main function of the HLA-G molecule in its membrane-bound and soluble forms is to inhibit the immune response by acting on CD4+ T cells, cytotoxic T cells, NK cells and dendritic cells. Lung cancer is a leading cause of death worldwide, and annual incidence is high in both women and men. Some studies have reported an increase of HLA-G serum levels in lung cancer, probably generated by tumor cells escaping the antitumor immune response. In this study the concentration of soluble HLA-G in bronchoalveolar lavage (BAL) in patients with primary and metastatic lung cancer was measured to determine its relation with tumor histological type and overall patient status according to the Karnofsky scale. METHODS: Thirty-one lung cancer patients were included. A tumor biopsy was obtained by bronchoscopy and the tumor type was determined by hematoxylin and eosin staining. BAL samples were obtained to measure soluble HLA-G concentrations in an ELISA sandwich assay. RESULTS: The average value of soluble HLA-G was 49.04ng/mL. No correlation between soluble HLA-G levels and age, gender or smoking was observed. A highly significant difference was observed in the levels of soluble HLA-G in BAL from patients with different histological types of lung cancer, especially in metastatic tumors. The Karnofsky index showed a significant and inverse correlation with soluble HLA-G levels in BAL. CONCLUSIONS: Soluble HLA-G protein is significantly associated with metastatic tumors and patients with lower Karnofsky index and may be useful as a prognostic marker in lung cancer.

Actividad antiviral y estudio de citotoxicidad de vinos tintos Sauvignon / Antiviral activity and citotoxicity study from Sauvignon red wines

Una variada actividad farmacológica del vino, relacionada con sus polifenoles como antioxidantes y antiviral, respecto a la cantidad. Se evaluó la actividad antiviral, respecto a la cantidad de polifenoles y su activación de tres vinos tintos mostraron un elevado contenido de polifenoles por afinidad con el azul de metileno. La actividad antiviral fue reconocida por la lisis de bacteriófagos de Pseudomonas aeruginosa. La electroforesis de poliacrilamida, mostró que los extractos de los vinos, con sulfato ferroso hidrolizaron las proteínas del fago. La toxicdad se evaluó en células mononucleares. La viabilidad de células mononucleares, fue superior a 76 por cient, demostrando baja toxicidad y un posible uso para estudios en humanos