[The indications of bronchial resection and anastomosis in lung cancer]. / Indicatii ale rezectiei bronhoanastomoza în neoplasmul bronhopulmonar.

Bronchial resection and anastomosis represents an alternative to the pneumonectomy in patients with severe impairment of pulmonary function and/or other diseases which make such radical surgery too risky. The authors present two clinical cases of right upper lobe lung cancer (a squamous-cell carcinoma and an adenocarcinoma) admitted in the Thoracic Surgery Department of the National Institute of Pulmonology "Marius Nasta" from Jan-March 2001, in which they could not perform pneumonectomy because of unacceptable high risks. In both patients a right upper lobectomy with "sleeve" resection was done, with the anastomosis of right main bronchus to the intermediary one. The clinical, bronchoscopic and functional results were excellent. The literature review also shows very good results of this technique in the surgical treatment of lung cancer. For these reasons, the authors recommend this procedure in all the cases in which it can be technically applied; more than that, the survival rate is similar with other more radical techniques, but without any complications.

[Secondary active-evolutive cavitary pulmonary tuberculosis of the apicodorsal segment of the left upper lobe associated with bronchial tuberculosis and bronchial geotrichosis]. / Tuberculoza pulmonara secundara activ-evolutiva cavitara segment apicodorsal lob superior stâng asociata cu tuberculoza bronsica si geotricoza bronsica.

A case of active pulmonary secondary tuberculosis associated with bronchial tuberculosis and bronchial geotrichosis is presented. This association is infrequent. Favoring factors: local and general immunodepression (recurrent infection with pyogenic germs, rubella, neutropenia, aggressive antibiotherapy, iron deficiency). The diagnosis was based mainly on the bacteriological sputum examination for bK (smear and culture) and the mycologic examination (smear and culture) of the bronchial aspiration, identifying Geotrichum candidum. The treatment consisted of antituberculous and antimycotic drugs, bronchodilators, bronchial aspirations and local endoscopic administration of antimycotic drugs.

[Evolution of the first hospital pharmacy in Saint-Spiridon hospital of Iasi]. / L'évolution de la pharmacie hospitalière "Saint-Spiridon" de Iasi, Roumanie.

The first Saint-Spiridon hospital was built in 1757. A pharmacy, managed by Andonie Feierman, was opened in 1765. After his death, foreign pharmacists succeeded one another until 1827, when was mentioned the first rumanian pharmacist, Ioan Padure. Teofil Stenner (1855-1890) held also the laboratory of chemistry and toxicology of Iasi. At the end of the XIXth century a new building was built, but it was destroyed during world war II.

Assay for lipolytic and proteolytic activity using marine substrates.

Nondestructive assay procedures for determining microbial lipolytic and proteolytic activity on marine substrates were developed and tested with 287 isolates of bacteria, filamentous fungi, and yeasts. A definite substrate specificity was noted when the enzymatic activities on marine and nonmarine substrates was compared. Of 170 lipolytic isolates, 14 were only active on menhaden oil, 11 could hydrolyze menhaden oil and Tween 80 and/or tributyrin, and 145 isolates could only hydrolyze one or both of the nonmarine lipids. Of the 198 proteolytic isolates, 10 were specific for codfish extract, 152 were active against the marine substrate plus casein and/or gelatin, and 36 were specific for nonmarine substrates.

Microflora of four fermented fish sauces.

Thirty-nine microorganisms representing 11 species of bacteria, one yeast, and three filamentous fungi were isolated and identified from four fermented fish sauces: nampla, patis, koami, and ounago.

Effects of aflatoxin on seedling growth and ultrastructure in plants.

Nineteen plants belonging to 11 species of the cruciferae were studied to determine the effects of aflatoxin B(1) on seed germination and seedling development. Germination was not inhibited in any test organism at a concentration of 100 mug of aflatoxin per ml of agar substrate. Inhibition of elongation of the hypocotyls and roots in the species studied varied from 29 to 93% and from 22 to 91% in the respective tissues. Lepidium sativum was the most susceptible plant studied and exhibited the maximal inhibitory response noted above at concentrations of 8 mug of aflatoxin per ml. The ultrastructure of Lepidium root cells treated with crystalline aflatoxin B(1) exhibited morphological changes characteristic of those found in aflatoxin-treated animal cells. In addition to changes in the cytoplasmic organelles, numerous ring-shaped nucleoli with prominent nucleolar caps were produced. The effect of aflatoxin on plant cells is compared with similar effects induced by actinomycin D. Seed germination and seedling development is discussed in relation to the effects of both compounds on deoxyribonucleic acid-dependent ribonucleic acid biosynthesis.

Effect of aflatoxin on germination and growth of lettuce.

The relative susceptibility of 30 cultivars of lettuce to inhibition by aflatoxin was studied. Seed germination was not inhibited by concentrations as high as 1,000 mug/ml in cultivar Imperial 44 or by 100 mug/ml in the remaining cultivars. Hypocotyl elongation was inhibited by 46 to 68% at a concentration of 100 mug of aflatoxin per ml. Seedlings exposed to aflatoxin did not become chlorotic. The similarity between the morphological reaction of plants to coumarin and aflatoxin suggests a common mode of action, but further studies of the physiological basis for the inhibitory reactions induced by these compounds will be necessary before such conclusions will be valid.

Fixation of mature spores of Clostridium botulinum.

A triple fixation method using a sequential application of 15 or 30% formaldehyde, 6% glutaraldehyde, and 1% osmium tetroxide resulted in excellent fixation of mature spores of Clostridium botulinum.