RESUMO
Twenty-nine classical swine fever virus (CSFv) strains were grown in the PK15 or SK6 cell lines. Antigenic differentiation studies were performed using monoclonal antibodies (McAbs), produced at Lelystad (CDI-DLO), The Netherlands. The monoclonals which were classified numerically as monoclonals 2-13. Epitope map patterns that resulted from the reactivity with McAbs were found to be unrelated to the pathogenicity of the viruses studied. Antigenic determinants were recognized by McAbs 5 and 8, were not detected in some Mexican strains; however, sites for McAb 6 were absent in all strains. The PAV-250 vaccine strain was recognized by all MAbs, except by MAb 6. Furthermore, the Chinese C-S vaccine strain was found to be very similar to the GPE(-) vaccine. None of the studied Mexican vaccines or field strains was found to be similar to the PAV-250 vaccine strain.
Assuntos
Antígenos Virais/análise , Vírus da Febre Suína Clássica/classificação , Vírus da Febre Suína Clássica/imunologia , Peste Suína Clássica/virologia , Animais , Anticorpos Monoclonais/imunologia , Anticorpos Antivirais/imunologia , Linhagem Celular , Vírus da Febre Suína Clássica/isolamento & purificação , Mapeamento de Epitopos , Epitopos/imunologia , México , SuínosRESUMO
Mycoplasmal pneumonia of swine causes considerable economic losses in porciculture. Diverse agents, such as environmental stress and infectious microorganisms, increase the possibility of infection, an the damage to pulmonary tissue when the infection is established. It is known that Mycoplasma hyopneumoniae has an important role in this desease, in addition to secondary microbial agents, susch as Pasteurella mutocida and Actinobacillus pleuropneumoniae. However, the characteristics of this disease in farms are well known. In this work we review the interactions among the different microorganisms involved and the immunological strategies utilized to control this disease. The interaction between Mycoplasma hyopneumoniae and P. multocida in experimental pneumonia was reported by us in conventional pig. M. hyopneumoniae causes mild pneumonia, whereas P. multocida aggravates the pneumonia initiated by M. hyopneumoniae. The disease has been reproduced to test the efficacy of two immunogens, and can also be used to evaluate some antibiotics. A M. hyopneumoniae bacterine administered intraperiotoneally conferred more protection than when it was used with adjuvant, although protection was not complete and colonization by M. hyopneumoniae was not prevented, as is claimed to have been the case with other vaccines
