RESUMO
Gastric cancer is one of the most common malignant tumors worldwide and has the second highest incidence and mortality rate among malignant tumors in China. Prostate-derived Ets factor (PDEF) is a member of the Ets family of transcription factors. Although PDEF plays an important role in tumorigenesis, its biological function in gastric cancer is still unclear. Here, we evaluated PDEF expression in 30 cases of human gastric carcinoma and the corresponding peritumoral tissues, using immunohistochemistry and immunofluorescence. Significantly higher levels of PDEF were detected in tumors compared to peritumoral tissues. We then investigated PDEF expression in the gastric cancer cell lines SGC and AGS and the normal gastric epithelial cell line GES; The CRISPR/Cas9 genome-editing system was used to knockout PDEF in AGS cells as a model for gastric cancer. Cell proliferation, apoptosis, migration, and invasion of PDEF-knockout AGS cells were evaluated using CCK-8, flow cytometry, scratch wound, and transwell assays, respectively. The results illustrated that PDEF-knockout inhibited AGS cell proliferation, migration, and invasion. Taken together, the results imply that PDEF plays important roles in the proliferation, migration, and invasion of AGS cells and may serve as a new treatment target in gastric cancer.
Assuntos
Sistemas CRISPR-Cas/fisiologia , Movimento Celular/fisiologia , Técnicas de Silenciamento de Genes/métodos , Invasividade Neoplásica/genética , Proteínas Proto-Oncogênicas c-ets/genética , Neoplasias Gástricas/genética , Sequência de Bases , Carcinogênese/genética , Carcinogênese/metabolismo , Linhagem Celular Tumoral , Mucosa Gástrica/metabolismo , Mucosa Gástrica/patologia , Humanos , Invasividade Neoplásica/patologia , Proteínas Proto-Oncogênicas c-ets/deficiência , Distribuição Aleatória , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/patologiaRESUMO
Fifteen soil samples were collected from Oncomelania hupensis culture pond in Miluo Schistosomiasis Control and Prevention Base, Hunan Province. Four strains of bacteria were identified to have molluscacidal effects, numbered as B8, B27, B36 and B59. Compared with the fermentation broth groups and bacteria suspension groups, the fermentation supernatant groups of the four strains showed the strongest molluscacidal effect. The fermentation supernatant of B59 strain showed the best molluscacidal effect, with snail mortalit of 73.3% and 96.7% at 48 h and 72 h of treatment, respectively. SDS-PAGE revealed no proteins in fermentation supernatant, fermentation broth and bacteria suspension of B59 strain. Molecular phylogenetic analysis based on ITS sequence showed that the ITS sequence of strain B59 (accession No. KP146144) was 100% homologous to that of the same fragment of Bacillus cereus (accession No. CP001746).
