On the use of the Cu2+-iminodiacetic acid complex for double histidine based distance measurements by pulsed ESR.

Cu2+ based distance measurements using the double-histidine (dHis) motif by pulsed ESR present an attractive strategy to obtain precise, narrow distance distributions that can be easily related to protein backbone structure (Cunningham et al., Angew. Chem., Int. Ed., 2015, 54, 633). The Cu2+-ion is introduced as a complex with the iminodiacetic acid (IDA) chelating agent, which enhances binding selectivity to the two histidine residues that are site-selectively placed on the protein through mutagenesis. However, initial results of this method produced weak dipolar modulations. To enhance applicability of the double histidine motif using IDA, we perform a systematic examination of the possible causes of these weak dipolar modulations. We examine the efficiency of the Cu2+-ion to form the Cu2+-IDA complex in solution. In addition, we analyze the selectivity of Cu2+-IDA binding to dHis sites at both α-helical and ß-strand environments. Our results indicate that the dHis motif on the ß-sheet sites have high affinity towards Cu2+-IDA while the dHis sites on α-helices show poor affinity for the metal-ion complex. We are able to use our new findings to optimize conditions to maximize dHis loading while minimizing both free Cu2+ and unbound Cu2+-IDA complex in solution, allowing us to double the sensitivity of the Double Electron-Electron Resonance (DEER) experiment. Finally, we illustrate how Cu2+-based CW-ESR and DEER can be combined to obtain information on populations of different Cu2+-complexes in solution.

The role of item distinctiveness in short-term recall of order information.

In two experiments, we examined short-term recall of order information using a partial-report distractor task. We manipulated the characteristics of a single letter in one of two four-letter segments. Participants knew in advance the identity of the letters in each segment. We made a single letter distinctive at presentation either by printing it in red or by replacing it with a red dash. Presenting the letter in red did not affect overall recall of the positions of the letters in the segment but did facilitate specific recall of the position of the distinct letter. Replacing the letter with a red dash inhibited overall recall as well as specific recall of the distinct letter. Participants were also less likely to respond in the regular output order when there was a dash replacing a letter in the segment. These effects of distinctiveness are explained in terms of output order processes in recent versions of the perturbation model.

Is there really very rapid forgetting from primary memory? The role of expectancy and item importance in short-term recall.

In two experiments, subjects recalled one of two letter segments following a digit-filled retention interval. In Experiment 1, recall expectancy was manipulated by using precues that correctly informed or misinformed subjects concerning which letter segment would be tested for recall. In Experiment 2, item importance was varied by precuing one segment as important but requiring that the uncued segment be recalled first. Recall performance was very low under conditions of low expectancy and low segment importance, but the slopes of the retention functions did not demonstrate more rapid forgetting than under standard conditions. The previous observations of very rapid forgetting from primary memory may be a function of an elevated initial recall level in the earlier studies. Our retention functions were compared with predictions of the Estes perturbation model. The findings suggested that when secondary memory processes were reduced, forgetting order information from primary memory occurred at the same rate as that estimated on the basis of previous studies using the standard distractor task.

A developmental evaluation of the role of word shape in word recognition.

Fourth- and seventh-grade children and college-age adults proofread passages typed either in lowercase or in all capital letters. Words were misspelled by deleting one of four letters, s, c, k, or p, that have similar features in lowercase and uppercase. Proofreading errors decreased with age and increasing reading ability, but all of the subjects were sensitive to changes in word shape--they missed more words with deletions of s or c than k or p in the lowercase passage but not in the all-capitals passage. These findings indicate that word shape is an important variable in recognizing familiar words, even for young readers.

Investigating the boundaries of reading units across ages and reading levels.

In Experiments 1 and 2 first-, third-, and seventh-grade children and college subjects circled the letter a while reading passages constructed of words familiar to first graders. First graders made more errors on the letter a embedded in a word than on the word a, whereas the converse was true of the other age groups. In Experiments 3 and 4 first-, second-, fourth-, and seventh-grade children and college students read passages and circled the letter t, making more errors on the common word the than on other words and on correctly spelled than on misspelled words. The effect of misspelling the other words increased with age and reading skill. Our combined results suggest that reading unit size increases with age and reading ability and that, whereas younger children, like adults, unitize common words, the unitization of less common words increases as word configurations become more familiar.

Association between familial sinistrality and pathological left-handedness: a comparison of mentally retarded and nonretarded subjects.

The hypothesis that familial sinistrality (FS) might be a useful variable for differentiating between pathological and natural left-handers was explored. A comparison of the incidence of FS in mentally retarded and nonretarded samples indicated that a positive history of familial sinistrality (FS+) was significantly more likely to occur in mildly retarded individuals. In addition, left-handed retarded people, in general, were significantly more likely to be FS+ than were right-handed retarded people.

Effects of cuing on short-term retention of order information.

In two experiments, subjects recalled one of two letter sequences following a digit-filled retention interval. Recall performance was increased by precues informing subjects which letter sequence would be tested, and the cuing advantage remained throughout 60-digit retention intervals. No improvement was found, however, for cues occurring after the letters but before the digits. The cuing effects were attributed to encoding, not rehearsal, processes and were explained by a version of the Estes perturbation model, which included a long-term storage component and a fixed perturbation probability.

Effects of repetition on short-term retention of order information.

Two experiments examined the effects of repetition on the short-term retention of order information. A partial report procedure was coupled with the distractor paradigm to vary which one of two 4-letter sequences was tested after a digit-filled retention interval. Repeatedly presenting the stimulus but not its testing did not increase recall, but recall did increase when both presentation and testing were repeated. Recall was also improved by precues that informed subjects in advance which sequence was to be recalled. The perturbation model of Estes (1972; Lee & Estes, 1981) was able to account for the qualitative and quantitative aspects of the results by making an explicit distinction between two types of rehearsal: the reactivation process, which corresponds to a passive, automatic rehearsal process; and an active, conscious rehearsal process that crystallizes the memory structure and makes perturbations less likely.

Delayed wound infection. An 11-year survey.

A small number of patients manifest wound infections several months to several years after their operations. A study was undertaken to delineate the clinical characteristics of patients whose infections became apparent after a prolonged time interval from surgery. Twenty-six patients were admitted to the New York Hospital-Cornell University Medical Center, with wound infections that occurred more than six months postoperatively. There were three distinct groups. The first group consisted of patients with Staphylococcus aureus infections related to superficial stitch abscesses. The second group comprised patients with pacemaker infections; S epidermidis was the most frequently recovered organism. The third group consisted of patients with more clinically significant infections. These infectious complications followed genitourinary, gastrointestinal, and biliary surgery. The findings of this study suggest that most of these infections are caused by organisms introduced into the wound at the time of surgery; these organisms may become active because of alterations in the host's resistance.