RESUMO
High intrathecal levels of anti-myelin basic protein (MBP) IgM were previously found to be significantly associated with early favorable course in a cohort of patients with multiple sclerosis (MS). A mAb to MBP 105-120 recognizing the 222-228 epitope of the extracellular domain of high affinity immunoglobulin gamma Fc-receptor I (CD64) was isolated from EBV(+) B cell clones of long-term stable RRMS patients. This mAb exerted immunosuppressive activity on MS-derived T cell lines through induction and release of high amounts of interleukin-10 and decreased levels of interleukin-12 from activated monocytes providing the biological basis for a potential new treatment for MS and other immune-mediated neurological disorders.
Assuntos
Anticorpos Monoclonais/farmacologia , Imunossupressores/farmacologia , Esclerose Múltipla/patologia , Proteína Básica da Mielina/imunologia , Receptores de IgG/metabolismo , Linfócitos T/efeitos dos fármacos , Adulto , Anticorpos Monoclonais/sangue , Linfócitos B/efeitos dos fármacos , Linfócitos B/imunologia , Linfócitos B/metabolismo , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Estudos de Coortes , Citocinas/genética , Citocinas/metabolismo , Proteínas de Ligação a DNA/imunologia , Ensaio de Imunoadsorção Enzimática , Mapeamento de Epitopos , Feminino , Citometria de Fluxo , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Imunoprecipitação , Imunossupressores/sangue , Masculino , Pessoa de Meia-Idade , Glicoproteína Mielina-Oligodendrócito/química , Glicoproteína Mielina-Oligodendrócito/imunologia , Peptídeos/imunologia , RNA Mensageiro/metabolismo , Receptores de IgG/genética , Estatísticas não Paramétricas , Linfócitos T/imunologia , Linfócitos T/metabolismo , Fatores de Transcrição/imunologia , TransfecçãoRESUMO
A total of 66.6% of Campylobacter pylori strains isolated from patients with peptic ulcers produced a cytotoxin active against mammalian cells in vitro, versus 30.1% of strains isolated from patients with chronic gastritis of various degrees of severity only. This difference was statistically significant and suggests that the toxic substance could be involved in the development of peptic ulcers.
Assuntos
Infecções por Campylobacter/microbiologia , Campylobacter/patogenicidade , Citotoxinas/biossíntese , Gastrite/microbiologia , Úlcera Péptica/microbiologia , Animais , Doença Crônica , Efeito Citopatogênico Viral , Células HeLa , Humanos , Células VeroRESUMO
Two groups of newborns on different diets were studied: group A was given artificial milk, while group B was breast fed. Blood samples from the cord artery and vein were taken at birth and further blood samples after 1, 6, 12, 24, 48, 72, 96 and 168 hours. No baby underwent more than three blood samples. Calcium (Ca), magnesium (Mg), phosphorus (P) and parathormone (PTH) were measured in all samples. There was a rapid increase in PTH levels in all subjects in the first hours after birth. At 48th the babies in group A had significantly higher serum levels of P than the group B subjects, and their serum Ca and Mg levels were significantly lower. At 72th PTH serum levels were significantly higher in group A, while Ca and Mg levels were significantly higher in group B. In conclusion, the subjects demonstrated a normal secretion of PTH at birth, and the type of diet notably influenced the homeostasis of calcium in the perinatal period.
Assuntos
Cálcio/sangue , Recém-Nascido , Magnésio/sangue , Hormônio Paratireóideo/sangue , Fósforo/sangue , Aleitamento Materno , Sangue Fetal/análise , Humanos , Alimentos InfantisRESUMO
Determinations of serum calcium (Ca), phosphorus (P) and calcitonin (CT) were carried out in two groups of full term newborn infants: 80 on bottle feeding (group A) and 80 breast feeding (group B). Samples of blood were drawn at 1, 6, 12, 24, 48, 72, 96 and 168 hours of life. Each newborn was tested no more than twice. No differences between the two groups could be detected before the 72th hour. At that time, the Ca of group A was significantly lower than Ca of group B while the CT and P were significantly higher in the same group A. So that, the P serum level appears to be one of the most important factor regulating the homeostasis of Ca after the first 72 hours of life.
