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1.
Int J Syst Evol Microbiol ; 51(Pt 2): 489-493, 2001 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11321095

RESUMO

Strain CT, a non-motile, mesophilic, hydrogenotrophic, methanogenic bacterium, was isolated from an anaerobic digester used for the treatment of raw cassava-peel waste in Congo. The cells were rods, 0.4-0.5 x 2-10 microm in size, and stained Gram-positive. Hydrogen and carbon dioxide were the only substrates that supported growth and methane production. Methane production, but not growth, occurred with CO2 in the presence of either 2-propanol, 2-butanol or cyclopentanol as hydrogen donors. The temperature range for growth was 25-50 degrees C, the optimum being between 37 and 42 degrees C. The optimum pH for growth was 7.2; consistent growth and methane production were not observed below pH 5.9 or above pH 8.2. The doubling time under optimal growth conditions was 7.5 h. The DNA base composition was 39.5 mol% G+C. On the basis of 16S rRNA gene sequence analysis and phenotypic characteristics, the isolate is proposed as a new species of the genus Methanobacterium, namely Methanobacterium congolense sp. nov. The type strain is strain CT (= DSM 7095T = OCM 779T).


Assuntos
Fermentação , Manihot/microbiologia , Methanobacterium/classificação , Reatores Biológicos , DNA Ribossômico , Manipulação de Alimentos , Methanobacterium/isolamento & purificação , Dados de Sequência Molecular , RNA Ribossômico 16S , Terminologia como Assunto
2.
Trop Med Int Health ; 4(8): 557-64, 1999 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-10499079

RESUMO

A large, randomized controlled trial to investigate the impact of insecticide-treated curtains (ITC) on child mortality was conducted in an area of seasonal, holoendemic malaria in Burkina Faso. 158 communities totalling some 90,000 people were censused and grouped into 16 geographical clusters, 8 of which were randomly selected to receive ITC in June-July 1994, just prior to the rainy season. In September-October 1995, at the peak period of malaria transmission, a cross-sectional survey was conducted in 84 of the villages. A random sample of 905 children aged 6-59 months was identified and visited. 763 children (84%) were present at the time of the visit and recruited into the study. Mothers were asked about fever in the past 24 h, the child's temperature was taken, and a sample of blood collected to identify and quantify malaria infections and to measure haemoglobin (Hb) levels. Children protected by ITC were less likely to be infected with Plasmodium falciparum (risk ratio = 0.92; 95% CI 0.86, 0.98) or P. malariae (risk ratio = 0.42, 95% CI 0.19, 0.95). The mean intensity of P. falciparum infections was lower among children protected by ITC (899 vs. 1583 trophozoites/microliter; P < 0.001), while the mean Hb level was 0.4 g/dl higher (P < 0.001). While we found no evidence that ITC had an impact on the prevalence of malaria-associated fever episodes, the confidence intervals around our estimates of the impact of ITC on malaria morbidity were wide. We conclude that widespread implementation of ITC in this area of high malaria transmission led to a modest reduction in the prevalence of malaria infection and to a more substantial reduction in the intensity of these infections which caused increased Hb levels. We were unable to demonstrate any impact of ITC on malaria morbidity, but the wide confidence intervals around our point estimates do not preclude the possibility of a substantial impact.


Assuntos
Roupas de Cama, Mesa e Banho , Inseticidas , Malária Falciparum/prevenção & controle , Malária/prevenção & controle , Plasmodium malariae/isolamento & purificação , Piretrinas , Distribuição por Idade , Animais , Burkina Faso/epidemiologia , Pré-Escolar , Estudos Transversais , Feminino , Febre/epidemiologia , Humanos , Lactente , Malária/epidemiologia , Malária Falciparum/epidemiologia , Masculino , Morbidade , Parasitemia/epidemiologia , Parasitemia/parasitologia , Parasitemia/prevenção & controle , Permetrina , Prevalência , Fatores de Risco , Estações do Ano
3.
Int J Syst Bacteriol ; 49 Pt 2: 639-43, 1999 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-10319487

RESUMO

A sulfate-reducing bacterium, strain HDvT (T = type strain), was isolated from an anoxic ricefield soil. Cells were Gram-negative, non-sporulating curved rods motile by means of a single polar flagellum. Cytochrome c3 and desulfoviridin were present. In the presence of sulfate, glycerol, 1,2- and 1,3-propanediol, dihydroxyacetone, pyruvate, lactate, fumarate, maleate, malate and succinate were incompletely oxidized mainly to acetate. Sulfite, thiosulfate, elemental sulfur, fumarate, maleate and malate were utilized as alternative electron acceptors. In the absence of added electron acceptors, pyruvate, fumarate, maleate, malate and dihydroxyacetone were fermented. The DNA base composition was 67 mol% G + C. The phylogenetic, phenotypic and physiological characteristics of strain HDvT indicate that it is a new species of the genus Desulfovibrio, for which the name Desulfovibrio burkinensis sp. nov. is proposed; the type strain is HDvT (= DSM 6830T). Phylogenetic analysis confirmed that Desulfovibrio alcoholivorans was a distinct species supporting the previously published phenotypic data.


Assuntos
Desulfovibrio/classificação , Desulfovibrio/isolamento & purificação , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , Burkina Faso , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Desulfovibrio/genética , Desulfovibrio/fisiologia , Genes de RNAr , Dados de Sequência Molecular , Oryza , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
4.
Arch Microbiol ; 158(3): 218-25, 1992.
Artigo em Inglês | MEDLINE | ID: mdl-1332638

RESUMO

A sulfate-reducing bacterium, strain HDv, was isolated from the anoxic soil of a ricefield using lactate as electron donor. Cells were gram-negative, motile, nonsporulating curved rods, with single polar flagella. Substrates were incompletely oxidized to acetate and included glycerol, 1,2- and 1,3-propanediol. Sulfate, sulfite, thiosulfate, elemental sulfur, fumarate, maleate, and malate were utilized as electron acceptors. Pyruvate, fumarate, maleate, malate and dihydroxyacetone were fermented. Desulfoviridin and c-type cytochromes were present. The DNA base composition was 66.6 +/- 0.3 mol% G+C. The isolate was identified as a Desulfovibrio sp.; its metabolic properties were somewhat different from those of previously described Desulfovibrio species. Comparative biochemical study of 1,2-propanediol dissimilation by the new isolate and Desulfovibrio alcoholovorans showed that NAD-dependent dehydrogenases play a key role in the catabolism of this substrate. The hypothetical pathways of 1,2-propanediol degradation by Desulfovibrio spp. are presented.


Assuntos
Desulfovibrio/enzimologia , Propilenoglicóis/metabolismo , Anaerobiose , Desulfovibrio/citologia , Desulfovibrio/isolamento & purificação , Desulfovibrio/fisiologia , Oxirredutases/metabolismo , Fosfotransferases/metabolismo , Propilenoglicol
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