Growth inhibition, oxidative stress and characterisation of mortality in green algae under the influence of beta-blockers and non-steroidal anti-inflammatory drugs.

Bisoprolol and ketoprofen are widely used pharmaceuticals in medical treatment hence these substances are occurring in wastewaters and in water environment. This research investigated the toxic effects of bisoprolol and ketoprofen on two microalgae taxa, Chlorella vulgaris and Desmodesmus armatus. The results showed that both drugs inhibited the growth of the species tested and induced a decrease in chlorophyll a content compared to controls. Ketoprofen turned out to be harmful to algae as the half maximal effective concentration (EC50) values (14 days) were 37.69 mg L-1 for C. vulgaris and 40.93 mg L-1 for D. armatus. On the other hand, for bisoprolol, the EC50 values were greater than the established NOEC, 100 mg L-1. Bisoprolol and ketoprofen induced oxidative stress in the tested microorganisms, as indicated by changes in the activities of antioxidant enzymes. Exposure to 100 mg L-1 of drugs significantly increased the activity of catalase, peroxidase and superoxide dismutase. Fluorescence microscopy showed that both medicaments changed the cells' morphology. There was atrophy of chlorophyll in the cells, moreover, dying multinuclear cells and cells without nuclei were observed. In addition, there were atrophic cells, namely cells that lacked nuclei and chlorophyll. Profile area analyses showed that bisoprolol and ketoprofen treated C. vulgaris cells were approximately 4 and 2 times greater compared to control ones. Our experimental findings highlight the ecotoxicological threats for aquatic primary producers from bisoprolol and ketoprofen and provide insight into the characteristics of their death.

Bisphenol A and its substitutes in the aquatic environment: Occurrence and toxicity assessment.

Bisphenol A is classified as a high production volume chemical commonly used in the manufacture of polycarbonate plastics, epoxy resins and thermal paper. The endocrine disrupting properties of this xenobiotic have led to the restriction and prohibition of its use in many consumer products. To date, many chemical compounds with a chemical structure similar to bisphenol A have been used in consumer products as its replacement. The ubiquitous occurrence of bisphenol A and its substitutes in the environment and their endocrine activity as well as adverse effects on aquatic organisms is a global concern, especially because many available literature reports show that many substitutes (e.g. bisphenol AF, bisphenol AP, bisphenol B, bisphenol C, bisphenol F, bisphenol G, bisphenol FL, tetrabromobisphenol A) exert adverse effects on aquatic organisms, similar to, or even stronger than bisphenol A. Therefore, the objective of this paper is to provide a comprehensive overview of the production, sources, occurrence and associated toxicity, as well as the endocrine activity of bisphenol A and its substitutes on aquatic species. The environmental levels and ecotoxicological data presented in this review allowed for a preliminary assessment and prediction of the risk of bisphenol A and its substitutes for aquatic organisms. Furthermore, the data collected in this paper highlight that several compounds applied in bisphenol A-free products are not safe alternatives and regulations regarding their use should be introduced.