RESUMO
The purpose of this study was to review and compare the properties of all the available cone beam CT (CBCT) devices offered on the market, while focusing especially on Europe. In this study, we included all the different commonly used CBCT devices currently available on the European market. Information about the properties of each device was obtained from the manufacturers' official available data, which was later confirmed by their representatives in cases where it was necessary. The main features of a total of 47 CBCT devices that are currently marketed by 20 companies were presented, compared and discussed in this study. All these CBCT devices differ in specific properties according to the companies that produce them. The summarized technical data from a large number of CBCT devices currently on the market offer a wide range of imaging possibilities in the oral and maxillofacial region.
Assuntos
Tomografia Computadorizada de Feixe Cônico/instrumentação , Césio , Tomografia Computadorizada de Feixe Cônico/economia , Custos e Análise de Custo , Desenho de Equipamento , Europa (Continente) , Humanos , Processamento de Imagem Assistida por Computador , Imageamento Tridimensional/economia , Imageamento Tridimensional/instrumentação , Iodetos , Posicionamento do Paciente , Doses de Radiação , Rotação , Fatores de Tempo , Tomógrafos Computadorizados/economiaRESUMO
A problem in many sequencing projects is the final closure of gaps left in the clone libraries, which serve as templates for sequencing, because of uncloned or unclonable genomic areas. By use of the Xylella fastidiosa genome as a test system, we present here an approach to generate, in a directed manner, sequence information from those gaps. We suggest using the complete clone library as a competitor against the genomic DNA of interest in a subtractive hybridization procedure similar to representational difference analysis (RDA). The resulting sequence information can be used to screen selectively other clone resources or serve directly for gap closure.
Assuntos
Análise de Sequência de DNA/métodos , DNA Bacteriano/genética , Genoma Bacteriano , Biblioteca Genômica , Hibridização de Ácido Nucleico/métodos , Xanthomonas/genéticaRESUMO
A cosmid library was made of the 2.7 Mb genome of the Gram-negative plant pathogenic bacterium Xylella fastidiosa and analysed by hybridisation mapping. Clones taken from the library as well as genomic restriction fragments of rarely cutting enzymes were used as probes. The latter served as a backbone for ordering the initial map contigs and thus facilitated gap closure. Also, the co-linearity of the cosmid map, and thus the eventual sequence, could be confirmed by this process. A subset of the eventual clone coverage was distributed to the Brazilian X.FASTIDIOSA: sequencing network. Data from this effort confirmed more quantitatively initial results from the hybridisation mapping that the redundancy of clone coverage ranged between 0 and 45-fold across the genome, while the average was 15-fold by experimental design. Reasons for this not unexpected fluctuation and the actual gaps are being discussed, as is the use of this effect for functional studies.