Restraining Staphylococcus aureus Virulence Factors and Quorum Sensing through Lactic Acid Bacteria Supernatant Extracts.

The escalating prevalence of antibiotic-resistant bacteria poses a grave threat to human health, necessitating the exploration of novel alternatives to conventional antibiotics. This study investigated the impact of extracts derived from the supernatant of four lactic acid bacteria strains on factors contributing to the pathogenicity of three Staphylococcus aureus strains. The study evaluated the influence of lactic acid bacteria supernatant extracts on the growth, biofilm biomass formation, biofilm metabolic activity, and biofilm integrity of the S. aureus strains. Additionally, the impact on virulence factors (hemolysin and coagulase) was examined. Gas chromatography coupled with mass spectrometry was used to identify the bioactive compounds in the extracts, while molecular docking analyses explored potential interactions. Predominantly, the extracts contain eight 2,5-diketopiperazines, which are cyclic forms of peptides. The extracts demonstrated inhibitory effects on biofilm formation, the ability to disrupt mature biofilms, and reduce the biofilm cell metabolic activity of the S. aureus strains. Furthermore, they exhibited the ability to inhibit α-hemolysin production and reduce coagulase activity. An in silico docking analysis reveals promising interactions between 2,5-diketopiperazines and key proteins (SarA and AgrA) in S. aureus, confirming their antivirulence and antibiofilm activities. These findings suggest that 2,5-diketopiperazines could serve as a promising lead compound in the fight against antibiotic-resistant S. aureus.

A cross-sectional study on the quality of life and psychosocial risk of migrant workers. / Estudio transversal sobre la calidad de vida y el riesgo psicosocial de trabajadores migrantes.

Introduction: Migration figures place Chile as one of the South American countries with the highest rate of migrants. The present study estimated the relationship between sociodemographic characteristics, quality of life, and psychosocial occupational risks in migrant workers from the Maule region. Methods: Cross-sectional study with migrant workers between 18 and 60 years of age residing in the Maule region (n = 145). The applied questionnaires were: a psychosocial risk questionnaire, a health and quality of life questionnaire, and a sociodemographic questionnaire. A bivariate statistical analysis was performed using nonparametric Mann-Whitney U tests, Kruskal Wallis, Spearman correlation, and multiple linear regression models. Results: In Chile, 21% of the migrants maintained the same work activity as in their country of origin. Although the quality of life in physical and mental health is adequate, 52% have low psychological demands at work, 48.9% have low levels of active work and development skills, 57.7% have a high-risk level of compensation and self-esteem, and 65.5% have a high-risk level of double presence at work. Migrants with a higher quality of life in the physical health dimension have a lower risk of maintaining a balance between effort and reward; they worked 44 hours a week and did not work directly at Maule. Migrants with a higher quality of life in the mental health dimension have a lower risk of emotional demands, perceive greater social support in the company, and are less concerned about domestic chores. Conclusions: Migrants with lower quality of life in the physical health dimension presented less compensation at work and recognition, came to work directly in the region, and had jobs with fewer contract hours. Workers with lower quality of life in their mental health exhibited a greater risk of psychological demands at work and perceived low social support in the company; they were concerned about having to respond to domestic and salaried work.

Introducción: Las cifras migratorias sitúan a Chile como uno de los países sudamericanos con mayor número de migrantes. El presente estudio estimó la relación entre características sociodemográficas, calidad de vida y riesgos psicosociales laborales en migrantes trabajadores de la región del Maule. Métodos: Estudio transversal con trabajadores migrantes entre 18 y 60 años, residentes en la Región del Maule (n = 145). Las encuestas aplicadas fueron: Cuestionario de riesgo psicosocial, Cuestionario de salud y calidad de vida y Cuestionario sociodemográfico. Se realizó un análisis estadístico bivariado con pruebas no paramétricas de U de Mann Whitney, Kruskal Wallis, correlación de Spearman y modelos de regresión lineal múltiple. Resultados: Un 21% de los migrantes mantuvo en Chile la misma actividad laboral a la que se dedicaban en su país de origen. Si bien la calidad de vida de la salud física y mental es adecuada, 52% presenta bajas demandas psicológicas en el trabajo, 48,9% bajos niveles de trabajo activo y habilidades de desarrollo, 57,7% tiene un nivel de alto riesgo en la compensación y la autoestima, y 65,5% un nivel de alto riesgo de doble presencia en el trabajo. Los migrantes con mayor calidad de vida en la dimensión salud física presentan menor riesgo de mantener el equilibrio entre el esfuerzo y recompensa, poseen un trabajo de 44 horas a la semana y no llegaron a trabajar directamente al Maule. Los migrantes con mayor calidad de vida en la dimensión salud mental poseen menor riesgo en las demandas emocionales, perciben mayor apoyo social en la empresa y menor preocupación por las tareas domésticas. Conclusiones: Los migrantes con menor calidad de vida en la dimensión salud física, presentan menores compensaciones en el trabajo, bajo reconocimiento, llegaron directamente a trabajar a la región y poseían trabajos con menos horas de contrato. Los trabajadores con menor calidad de vida en su salud mental exhiben mayor riesgo en las exigencias psicológicas en el trabajo, perciben bajo apoyo social en la empresa y preocupación por responder al trabajo doméstico y al asalariado.

Estudio transversal sobre la calidad de vida y el riesgo psicosocial de trabajadores migrantes / A cross-sectional study on the quality of life and psychosocial risk of migrant workers

INTRODUCIÓN: Las cifras migratorias sitúan a Chile como uno de los países sudamericanos con mayor número de migrantes. El presente estudio estimó la relación entre características sociodemográficas, calidad de vida y riesgos psicosociales laborales en migrantes trabajadores de la región del Maule. MÉTODOS: Estudio transversal con trabajadores migrantes entre 18 y 60 años, residentes en la Región del Maule (n = 145). Las encuestas aplicadas fueron: Cuestionario de riesgo psicosocial, Cuestionario de salud y calidad de vida y Cuestionario sociodemográfico. Se realizó un análisis estadístico bivariado con pruebas no paramétricas de U de Mann Whitney, Kruskal Wallis, correlación de Spearman y modelos de regresión lineal múltiple. RESULTADOS: Un 21% de los migrantes mantuvo en Chile la misma actividad laboral a la que se dedicaban en su país de origen. Si bien la calidad de vida de la salud física y mental es adecuada, 52% presenta bajas demandas psicológicas en el trabajo, 48,9% bajos niveles de trabajo activo y habilidades de desarrollo, 57,7% tiene un nivel de alto riesgo en la compensación y la autoestima, y 65,5% un nivel de alto riesgo de doble presencia en el trabajo. Los migrantes con mayor calidad de vida en la dimensión salud física presentan menor riesgo de mantener el equilibrio entre el esfuerzo y recompensa, poseen un trabajo de 44 horas a la semana y no llegaron a trabajar directamente al Maule. Los migrantes con mayor calidad de vida en la dimensión salud mental poseen menor riesgo en las demandas emocionales, perciben mayor apoyo social en la empresa y menor preocupación por las tareas domésticas. CONCLUSIONES: Los migrantes con menor calidad de vida en la dimensión salud física, presentan menores compensaciones en el trabajo, bajo reconocimiento, llegaron directamente a trabajar a la región y poseían trabajos con menos horas de contrato. Los trabajadores con menor calidad de vida en su salud mental exhiben mayor riesgo en las exigencias psicológicas en el trabajo, perciben bajo apoyo social en la empresa y preocupación por responder al trabajo doméstico y al asalariado.

INTRODUCTION: Migration figures place Chile as one of the South American countries with the highest rate of migrants. The present study estimated the relationship between sociodemographic characteristics, quality of life, and psychosocial occupational risks in migrant workers from the Maule region. METHODS: Cross-sectional study with migrant workers between 18 and 60 years of age residing in the Maule region (n = 145). The applied questionnaires were: a psychosocial risk questionnaire, a health and quality of life questionnaire, and a sociodemographic questionnaire. A bivariate statistical analysis was performed using nonparametric Mann-Whitney U tests, Kruskal Wallis, Spearman correlation, and multiple linear regression models. RESULTS: In Chile, 21% of the migrants maintained the same work activity as in their country of origin. Although the quality of life in physical and mental health is adequate, 52% have low psychological demands at work, 48.9% have low levels of active work and development skills, 57.7% have a high-risk level of compensation and self-esteem, and 65.5% have a high-risk level of double presence at work. Migrants with a higher quality of life in the physical health dimension have a lower risk of maintaining a balance between effort and reward; they worked 44 hours a week and did not work directly at Maule. Migrants with a higher quality of life in the mental health dimension have a lower risk of emotional demands, perceive greater social support in the company, and are less concerned about domestic chores. CONCLUSIONS: Migrants with lower quality of life in the physical health dimension presented less compensation at work and recognition, came to work directly in the region, and had jobs with fewer contract hours. Workers with lower quality of life in their mental health exhibited a greater risk of psychological demands at work and perceived low social support in the company; they were concerned about having to respond to domestic and salaried work.

Human probiotic bacteria attenuate Pseudomonas aeruginosa biofilm and virulence by quorum-sensing inhibition.

This work investigated chloroform extracts from culture supernatants of two human probiotic bacteria, Lactobacillus casei CRL 431 and Lactobacillus acidophilus CRL 730 for the production of virulence factors and quorum sensing (QS) interference against three Pseudomonas aeruginosa strains. Both extracts inhibited biofilm biomass (up to 50%), biofilm metabolic activity (up to 39%), the production of the enzyme elastase (up to 63%) and pyocyanin (up to 77%), and decreased QS, without presenting any antibacterial acgivity. In addition, the chloroform extracts of both strains disrupted preformed biofilms of the three strains of P. aeruginosa analyzed (up to 40%). GC-MS analysis revealed that the major compounds detected in the bioactive extracts were four diketopiperazines. This study suggests that the metabolites of L. casei and L. acidophilus could be a promising alternative to combat the pathogenicity of P. aeruginosa.

Bioactive Heterometallic CuII-ZnII Complexes with Potential Biomedical Applications.

A series of multinuclear heterometallic Cu-Zn complexes of molecular formula [(CuL)2Zn(dca)2] (1), [(CuL)2Zn(NO3)2] (2), [(CuL)2Zn2(Cl)4] (3), and [(CuL)2Zn2(NO2)4] (4) have been synthesized by reacting [CuL] as a "metalloligand (ML)" (where HL = N,N'-bis(5-chloro-2-hydroxybenzylidene)-2,2-dimethylpropane-1,3-diamine) and by varying the anions or coligands using the same molar ratios of the reactants. All of the four products including the ML have been characterized by infrared and UV-vis spectroscopies and elemental and single-crystal X-ray diffraction analyses. By varying the anions, different structures and topologies are obtained which we have tried to rationalize by means of thorough density functional theory calculations. All of the complexes (1-4) have now been applied for several biological investigations to verify their therapeutic worth. First, their cytotoxicity properties were assessed against HeLa human cervical carcinoma along with the determination of IC50 values. The study was extended with extensive DNA and protein binding experiments followed by detailed fluorescence quenching study with suitable reagents to comprehend the mechanistic pathway. From all of these biological studies, it has been found that all of these heterometallic complexes show more than a few fold improvement of their therapeutic values as compared to the similar homometallic ones probably because of the simultaneous synergic effect of copper and zinc. Among all of the four heterometallic complexes, complex 3 exhibits highest binding constants and IC50 values suggest for their better interaction toward the biological targets and hence have better clinical importance.

Association among polymorphisms in the steroid 5alpha-reductase type II (SRD5A2) gene, prostate cancer risk, and pathologic characteristics of prostate tumors in an Ecuadorian population.

Androgens are essential to normal prostate growth and development. It is therefore possible that polymorphisms in the androgen synthesis gene 5alpha-reductase type II (SRD5A2) may be involved in the progression of prostate tumors. We evaluated the relationship of two single-nucleotide polymorphisms, A49T and V89L, with prostate cancer risk in a case-control study. A total of 114 prostate cancer patients and 144 healthy control males were genotyped. We found highly significant differences between the two polymorphisms, the risk of developing prostate cancer, and some of the clinical-pathologic characteristics. Individuals who carry at least one V allele may have a higher risk of developing prostate cancer [odds ratio (OR) = 7.5, 95% confidence interval (CI) = 2.57-22.08, P<0.001]. In addition, individuals with LL genotype showed reduction in the progression to a higher tumor stage (OR = 0.10, 95%CI = 0.040-0.27, P<0.001). The A49T substitution was associated with a higher pTNM stage (OR = 2.87, 95%CI 1.14-7.21, P = 0.003) and elevated Gleason grade (OR = 3.14, 95%CI = 1.12-8.78; P = 0.004). Furthermore, the allelic frequencies of the A49T variant (33% controls and 45% cases) are the highest reported worldwide. These findings suggest that among the Ecuadorian population, these polymorphisms influence the risk of developing prostate cancer.

Factores asociados con lesiones neoplásicas del tracto genital inferior femenino en adolescentes del departamento de Caldas / Fatores associados com lesões neoplásticas do conduto genital inferior feminino em adolescentes do departamento de Caldas / Factors associated with neoplasm of the lower female genital tract in teens in the department of Caldas

En este artículo se presentan los resultados de un estudio epidemiológico analítico-descriptivo, cuyos objetivos fueron: conocer y determinar cómo se asocian factores biológicos y psicosociales con la presencia de lesiones neoplásicas del tracto genital inferior femenino en una población adolescente del departamento de Caldas, lo cual se relacionó, por un lado, con el análisis de la distribución y frecuencia de la población estudiada en cuanto a sexualidad, reproducción, factores culturales y factores psicosociales, y por otro, con la interacción de factores de riesgo en la aparición de dichas lesiones. Se tomó una muestra de 269 mujeres, seleccionadas después de su participación en jornadas educativas respecto a cáncer de cuello uterino y de mama. A este grupo se le aplicó una encuesta estructurada, diseñada para tal fin, la cual se diligenció directamente por los investigadores, previo consentimiento informado. A cada informante se le realizó citología y colposcopia, con un manejo estrictamente confidencial de la información. Los datos se analizaron retomando su distribución y frecuencia por categorías de variable, con lo cual se buscó determinar la asociación de los factores con la presencia o no de lesiones neoplásicas al contrastarlos con los resultados de citología y colposcopia.

This article presents the results of an epidemiologic analytical-descriptive study whose objectives were to examine and determine how biological and psychosocial factors are associated to the presence of neoplastic lesions of the lower female genital tract in a teenage population in the department of Caldas. The study analyzed the distribution and frequency of the studied population in terms of sexuality, reproduction, cultural and psychosocial factors, and secondly, the interaction of risk factors in the occurrence of such injury. A sample of 269 women, selected after their involvement in educational sessions related to cervical cancer and breast cancer, was interviewed by means of a structured survey, which was filled-out directly by the researchers, with prior consent from the women. A cervical smear and a colposcopy were performed on each patient, confidentially handling the information. The data was analyzed taking into consideration the distribution and frequency of the variable categories, looking to determine the association of factors with the presence or absence of neoplastic lesions, when contrasted with the cervical smear and colposcopy results.

Em este artigo apresentam se os resultados duma pesquisa epidemiológica analítico-descritivo, cujos objetivos foram: conhecer e determinar como associam se fatores biológicos y psicossociais com a presença de lesões neoplásicas do conduto genital inferior feminino numa povoação adolescente do Departamento de Caldas, o qual se relacionou, por um lado, com a análise da distribuição e freqüência da povoação estudada em quanto a sexualidade, reprodução, fatores culturais e fatores psicossociais, e por outro lado, com a interação de fatores de risco na aparição de ditas lesões. Tomou se uma amostra de 269 mulheres, selecionadas depois de sua participação em jornadas educativas respeito ao câncer de colo uterino e mama. A este grupo aplicou se uma enquete estruturada, desenhada tal fim, a qual diligenciou se direitamente por os pesquisadores, prévio consentimento informado. A cada informante realizou se citologia e colposcopia, com um manejo estritamente confidencial da informação. Os dados realizaram se retomando sua distribuição e freqüência por categorias de variável, com o qual se procurou determinar a associação dos fatores com a presença ou não de lesões neoplásicas ao contrastarlos com os resultados de citologias e colposcopias.

Low concentrations of zinc in gastric mucosa are associated with increased severity of Helicobacter pylori-induced inflammation.

BACKGROUND: Chronic Helicobacter pylori infection is the most common cause of gastric cancer. H. pylori induces oxidative stress while zinc deficiency results in increased sensitivity to it. In Ecuador, the prevalence of gastric cancer and zinc deficiency are high. We hypothesized that zinc deficiency in Ecuadorian people would cause increased H. pylori-induced inflammation in the gastric mucosa associated with lower tissue zinc concentrations. METHODS: Three hundred and fifty-two patients with dyspepsia underwent endoscopy to obtain gastric mucosa biopsies. Diagnosis of H. pylori infection and its severity, histopathology, mucosal zinc concentration, and inflammation intensity were determined. RESULTS: H. pylori-infected patients with non-atrophic chronic gastritis had lower concentrations of zinc in gastric mucosa than uninfected patients with the same type of gastritis (251.3 +/- 225.3 vs. 426.2 +/- 279.9 ng/mg of protein; p = .016). Considering all patients, the more severe the H. pylori infection, the higher the percentage of subjects with infiltration by polymorphonuclear (PMN) cells (p = .0001). Patients with high PMN infiltration had lower mucosal zinc concentrations than patients with low PMN infiltration (35.2 +/- 20.7 vs. 242.9 +/- 191.8 ng/mg of protein; p = .021). CONCLUSIONS: The degree of inflammation in H. pylori-induced gastritis appears to be modulated by gastric tissue zinc concentrations.