Effects of electrolyte and glycerol supplementation on recovery from endurance exercise.

Incomplete recovery from endurance exercise after an overnight rest period is reflected by persisting weight loss and an elevated plasma aldosterone concentration, even in successful competitors. To determine whether supplementation with high doses of electrolytes, with or without glycerol, enhances recovery, the following were measured in 6 Arabian horses before and after completion of a 60 km treadmill exercise test simulating an endurance ride and after 12, 24, 48, and 72 h of recovery: bodyweight; plasma osmolality; plasma concentrations of protein, electrolytes, aldosterone and cortisol; and urine and faecal electrolyte concentrations. Before and during the exercise test, horses were supplemented with a total of 2.4 ml/kg bwt of water (W); 0.2 g/kg bwt KCl and 0.4 g/kg bwt NaCl in 2.4 ml/kg bwt of water (E); or 0.2 g/kg bwt KCl and 0.4 g/kg bwt NaCl in 2.4 ml/kg bwt (3 g/kg bwt) of glycerol (GE). Although weight loss after completion of the simulated ride was greater (P < 0.01) for W (3.2%) than for E and GE (1.0 and 0.9%, respectively), horses supplemented with E or GE experienced further weight loss by 24 h after the simulated ride (2.2 and 2.1% for E and GE, respectively) while bodyweight with W remained unchanged (3.0%) from the finish value. After 48 h of recovery, bodyweight was not different from the starting values with E and GE but remained decreased (P < 0.01) with W throughout the recovery period (2.2% persisting weight loss after 72 h of recovery). Plasma osmolality and plasma Na+ and Cl- concentrations increased (P < 0.01) and plasma protein concentration decreased (P < 0.01) after the exercise test with E and GE but were unchanged with W. Plasma osmolality and protein and electrolyte concentrations returned to pre-exercise values within 12 h of recovery with the exception of a persistent increase in plasma Na+ concentration with GE. The greatest plasma aldosterone concentration was measured after 12 h of recovery with W (1357 pmol/l) and was greater (P < 0.02) than that with E and GE (24 and 304 pmol/l, respectively). Urine production during the simulated ride increased (P < 0.01) with GE and resulted in loss of approximately 20% and essentially 100% of supplemented Na+ and K+, respectively. In contrast, electrolyte losses in faeces were unaffected by electrolyte or glycerol supplementation. In conclusion, supplementation with high doses of electrolytes as hypertonic oral pastes attenuated weight loss during a simulated endurance ride (by enhancing voluntary water intake); however, it did not prevent development of significant weight loss during the initial 24 h of recovery. Glycerol administration resulted in no benefits, and actually increased urine electrolyte losses, in comparison to supplementation with electrolytes alone.

Electrolyte and glycerol supplementation improve water intake by horses performing a simulated 60 km endurance ride.

To replace electrolytes lost in sweat during endurance competitions, riders frequently supplement horses with hypertonic oral electrolyte pastes. To determine whether this practice and concurrent administration of the so-called hyperhydrating substance glycerol are of benefit, weight loss, voluntary water intake, plasma osmolality, and plasma protein and electrolyte concentrations were measured in 6 Arabian horses supplemented with a total of 2.4 ml/kg bwt of water (W); 0.2 g/kg bwt KCl and 0.4 g/kg bwt NaCl in 2.4 ml/kg bwt of water (E); or 0.2 g/kg bwt KCl and 0.4 g/kg bwt NaCl in 2.4 ml/kg bwt (3 g/kg bwt) of glycerol (GE) before and during a treadmill exercise test simulating a 60 km endurance ride. Weight loss was greater (P < 0.01) with W (3.2%) than with E and GE (1.0% and 0.9%, respectively) and was associated (r = -0.85, P < 0.0001) with less (P < 0.01) water intake with W (12.2 l) than with E or GE (23.5 l and 25.8 l, respectively). Plasma osmolality increased to a greater extent (P < 0.01) with GE than with E and was unchanged with W. In contrast, plasma protein concentration decreased (P < 0.01) in the later stages of the simulated ride with E and GE, reflecting plasma volume expansion, but remained unchanged with W. Plasma Na+ and Cl- concentrations increased (P < 0.01) with E and GE and were greater (P < 0.01) than values for W during the second half of the 60 km simulated ride. Despite administration are large amounts of potassium with E and GE, plasma K+ concentration was decreased (P < 0.01) at the end of the rest breaks during the simulated ride and after 60 min of recovery with all supplements. Adverse effects of administration of hypertonic oral electrolyte pastes were not observed and decreases in plasma protein concentration within 30 min after drinking with all supplements suggested that intestinal absorption was well maintained during the simulated endurance ride. In conclusion, electrolyte supplementation in the form of hypertonic oral pastes improved water intake during endurance exercise but concurrent glycerol administration provided no additional benefits in comparison to supplementation with electrolytes alone.