Recent treatment progress of triple negative breast cancer.

Breast cancer (BC) is a serious worldwide disease that threatens women's health. Particularly, the morbidity of triple-negative breast cancer (TNBC) is higher than that of other BC types due to its high molecular heterogeneity, metastatic potential and poor prognosis. TNBC lacks of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2), so there are still no effective treatment methods for TNBC. Here, we reviewed the classification of TNBC, its molecular mechanisms of pathogenesis, treatment methods and prognosis. Finding effective targets is critical for the treatment of TNBC. Also, refining the classification of TNBC is benefited to choose the treatment of TNBC, because the sensitivity of chemotherapy is different in different TNBC. Some new treatment methods have been proposed in recent years, such as nutritional therapy and noncoding RNA treatment methods. There are some disadvantages, such as the side effect on normal cells after nutrient deprivation, low specificity and instability of noncoding RNA. More studies are necessary to improve the treatment of TNBC.

Enhanced remediation of bispyribac sodium by wheat (Triticum aestivum) and a bispyribac sodium degrading bacterial consortium (BDAM).

The use of plant-bacterial association is a promising approach for the enhanced remediation of pesticides. Generally, both rhizo- and endosphere bacteria assist their host plants to survive in the contaminated environment. In this work, we have studied the individual and combined effects of wheat (Triticum aestivum) and a previously optimized bispyribac sodium (BS) degrading bacterial consortium (BDAM) on the degradation of BS and plant biomass production. Results showed that the bacterial strains of the BDAM have successfully survived in the plant rhizo-as well as endosphere and enhanced degradation of BS and plant biomass. In soil spiked with 2 mg/kg and 5 mg/kg of BS and was planted and inoculated with BDAM (P_I) showed 100% degradation of BS both in rhizosphere soil and endosphere of the plant. However, during the same period (45 days) the degradation of BS was 96 and 90%, and 93 and 84% in inoculated but un-planted (I_UP) and planted but un-inoculated (P_UI) soils spiked with 2 and 5 mg/kg, respectively. Liquid chromatography-mass spectrometry (LC-MS) analysis of the treated samples showed novel degradation products of BS. Based on the results, we concluded that plant-bacterial association is an efficient tool for enhanced remediation of BS contaminated soil and herbicide free crop production.

Application of a novel bacterial consortium BDAM for bioremediation of bispyribac sodium in wheat vegetated soil.

Plant-bacterial mutualism has tremendous potential for remediation of herbicide contaminated soils. Generally, bacterial inoculation helps plants to grow well in the contaminated environment. Here, we investigated the impact of bispyribac sodium (BS) degrading bacterial consortium (BDAM) on BS remediation, plant growth promotion and BS accumulation in plant parts. Wheat (Triticum aestivum) was planted in BS spiked soil and inoculated with BDAM. Inoculation showed a beneficial effect on plant biomass production and degradation of BS in the rhizosphere and the rhizosheath. After 40 and 60 days of inoculation, the degradation of BS was more than 96% and approximately 100% respectively in the planted and inoculated soil spiked with 2 and 5 mg kg-1 BS. However, in planted and un-inoculated soil, the degradation of BS was 72% after 60 days of sowing. Furthermore, inoculated bacterial strains colonized both in rhizo- and endosphere of the inoculated plants. In comparison with the un-inoculated soil, significantly less accumulation of BS was found in the roots and shoots of the plants growing in inoculated soil. We report the efficiency of plant-bacterial partnership for enhanced biodegradation of BS and to eliminate the BS residual toxicity to non-target plants.

Biodegradation of bispyribac sodium by a novel bacterial consortium BDAM: Optimization of degradation conditions using response surface methodology.

Bispyribac sodium (BS), is a selective, systemic and post emergent herbicide used to eradicate grasses and broad leaf weeds. Extensive use of this herbicide has engendered serious environmental concerns. Hence it is important to develop strategies for bioremediation of BS in a cost effective and environment friendly way. In this study a bacterial consortium named BDAM, comprising three novel isolates Achromobacter xylosoxidans (BD1), Achromobacter pulmonis (BA2), and Ochrobactrum intermedium (BM2), was developed by virtue of its potential for degradation of BS. Different culture conditions (temperature, pH and inoculum size) were optimized for degradation of BS by the consortium BDAM and the mutual interactions of these parameters were analysed using a 23 full factorial central composite design (CCD) based on Response Surface Methodology (RSM). The optimal values for temperature, pH and inoculum size were found to be 40 °C, 8 and 0.4 g/L respectively to achieve maximum degradation of BS (85.6%). Moreover, the interactive effects of these parameters were investigated using three dimensional surface plots in terms of maximum fitness function. Importantly, it was concluded that the newly developed consortium is a potential candidate for biodegradation of BS in a safe, cost-effective and environmentally friendly manner.