Dopamine-Functionalized, Red Carbon Quantum Dots for In Vivo Bioimaging, Cancer Therapeutics, and Neuronal Differentiation.

One of the crucial requirements of quantum dots for biological applications is their surface modification for very specific and enhanced biological recognition and uptake. Toward this end, we present the green synthesis of bright, red-emitting carbon quantum dots derived from mango leaf extract (mQDs). These mQDs are conjugated electrostatically with dopamine to form mQDs-dopamine (mQDs:DOPA) bioconjugates. Bright-red fluorescence of mQDs was used for bioimaging and uptake in cancerous and noncancerous cell lines, tissues, and in vivo models like zebrafish. mQDs exhibited the highest uptake in brain tissue compared to the heart, kidney, and liver. mQD:DOPA conjugates killed breast cancer cells and increased uptake in epithelial RPE-1 cells and zebrafish. Additionally, mQDs:DOPA promoted neuronal differentiation of SH-SY5Y cells to differentiated neurons. Both mQDs and mQDs:DOPA exhibited the potential for higher collective cell migrations, implicating their future potential as next-generation tools for advanced biological and biomedical applications.

Designing Cost-Effective Open-Source Multihead 3D Bioprinters.

For the past decade, additive manufacturing has resulted in significant advances toward fabricating anatomic-size patient-specific scaffolds for tissue models and regenerative medicine. This can be attributed to the development of advanced bioinks capable of precise deposition of cells and biomaterials. The combination of additive manufacturing with advanced bioinks is enabling researchers to fabricate intricate tissue scaffolds that recreate the complex spatial distributions of cells and bioactive cues found in the human body. However, the expansion of this promising technique has been hampered by the high cost of commercially available bioprinters and proprietary software. In contrast, conventional three-dimensional (3D) printing has become increasingly popular with home hobbyists and caused an explosion of both low-cost thermoplastic 3D printers and open-source software to control the printer. In this study, we bring these benefits into the field of bioprinting by converting widely available and cost-effective 3D printers into fully functional, open-source, and customizable multihead bioprinters. These bioprinters utilize computer controlled volumetric extrusion, allowing bioinks with a wide range of flow properties to be bioprinted, including non-Newtonian bioinks. We demonstrate the practicality of this approach by designing bioprinters customized with multiple extruders, automatic bed leveling, and temperature controls for ∼$400 USD. These bioprinters were then used for in vitro and ex vivo bioprinting to demonstrate their utility for tissue engineering.