[Efficacy of two bariatric surgeries in type 2 diabetic patients with a body mass index of 25-27.5].

OBJECTIVE: To evaluate the therapeutic effects of laparoscopic Roux-en-Y gastric bypass (LRYGB) and laparoscopic duodenal-jejunal bypass with sleeve gastrectomy (SADJB-SG) in patients with type 2 diabetes mellitus and a low body mass index (BMI) of 25-27.5. METHODS: Thirty-one type 2 diabetic patients with a BMI of 25-27.5 underwent bariatric surgeries in the General Hospital of Guangzhou Military Command between August, 2013 and August, 2015. The patients receiving LRYGB (17 cases) and SADJB-SG (14 cases) were compared for physical indexes, glucose metabolism and of pancreatic islet function at 1 year after the surgeries. RESULTS: No mortality occurred in the patients after the operations. At 1 year after the operation, the patients in LRYGB group showed significant improvements in body weight, BMI, glycated hemoglobin (HbA1c), fasting plasma glucose (FPG), oral glucose tolerance test 2 h (OGTT2h), C-peptide, fasting insulin (FINS), and postprandial 2 hour insulin (2 hPINS) (P<0.05); in SADJB-SG group, significant improvements were observed in the body weigh, BMI, HbA1c, FPG, OGTT2h, and FINS after the operation (P<0.05). The postoperative improvements in body weigh, BMI, HbA1c, FPG, OGTT2h, C-peptide, and 2hPINS were comparable between SADJB-SG group and LRYGB group (P>0.05), but the incidence of postoperative anastomotic ulcer was lower in SADJB-SG group. CONCLUSION: SADJB-SG and LRYGB produce similar therapeutic effects in type 2 diabetic patients with a low BMI, but SADJB-SG is associated with a low incidence of postoperative complications and is therefore more suitable in such patients.

Apoptosis of Spodoptera litura cells induced by AcMNPV ie-1 gene.

Apoptosis was induced in Spodoptera litura Sl-zsu-1 cells transfected with plasmid pAcie-1 DNA containing ie-1 gene of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV). Apoptotic bodies appeared 24 h post transfection and cell mortality peaked after 48 h. Electrophoresis of total DNA extract of Sl-zsu-1 cells showed a DNA ladder, indicating that transfection of ie-1 gene alone can trigger apoptosis directly or indirectly. It was shown by cytochalasin D (CD) and ammonium chloride inhibition assays that the entry of virions into cytoplasm was required for induction of apoptosis. Reverse transcriptase PCR (RT-PCR) was used to demonstrate that ie-1 gene had transcribed in Sl-zsu-1 cells immediately after infection. Inhibition of DNA replication could not block the cell death process, suggesting that this apoptosis was independent on viral late replication events. Apoptosis did not occur when Sl-zsu-1 cells were infected with an AcMNPV mutant tsB821 at its nonpermissive temperature. Therefore, we concluded that the ie-1 gene is at least one of the factors inducing apoptosis in Sl-zsu-1 cells infected with AcMNPV.