RESUMO
Innate immune response to a lipopolysaccharide (LPS) challenge varies among sheep breeds. How different breeds respond to bacterial infections impacts management practices of sheep producers. Hence, clinical response, acute-phase response, and gene expression of pro- and anti-inflammatory markers in peripheral white blood cells (WBCs) were examined after an LPS challenge in Dorset and Suffolk ewes. Ewes received either PBS or 2.5 µg/kg LPS (i.v.) 4 to 5 d after onset of synchronized estrus. Blood was collected via jugular venipuncture intermittently for 24 h to determine WBC counts. Rectal temperatures and observations of behavioral/physical appearances were recorded hourly. After LPS, WBCs decreased the first hour (P = 0.0001) and rectal temperatures (P < 0.0001) increased through 4 h; both returned toward normal 6 h after challenge. Suffolk ewes exhibited greater changes in temperature (P = 0.03) and behavioral/physical responses (P < 0.0001) than Dorset ewes and had an enhanced acute-phase response demonstrated by increased concentrations of plasma haptoglobin (P = 0.04), as well as cortisol concentrations (P = 0.03). Real-time PCR was completed on buffy coat homogenates for expression of pro-inflammatory [CXCL8, IL-6, interferon gamma (IFNG), complement component 3 (C3), toll-like receptor 4 (TLR4), prostaglandin synthase 2 (PTGS2)] and anti-inflammatory [IL-10, superoxide dismutase 2 (SOD2), forkhead box P3 (FOXP3), peroxisome proliferator-activated receptor gamma (PPARG), mannose receptor C type 1 (MRC1), transforming growth factor ß (TGFß)] genes. After LPS treatment, gene expressions increased for CXCL8 (P = 0.0003), TLR4 (P = 0.004), SOD2 (P < 0.0001), and C3 (P = 0.003), while PPARG (P = 0.006) and MRC1 (P = 0.003) decreased. Overall, Dorset ewes had greater expression of TLR4 (P = 0.003), IL-10 (P = 0.045), PPARG (P = 0.002), FOXP3 (P = 0.001), and SOD2 (P = 0.0002), whereas Suffolk ewes had greater expression of IL-6 (P = 0.0007), IFNG (P = 0.02), PTGS2 (P = 0.0002), and C3 (P = 0.008). Suffolk ewes also displayed greater expression of IL-6 (P = 0.002) and C3 (P = 0.0004) in response to LPS. In conclusion, differences in gene expression may explain the enhanced inflammatory response in Suffolk ewes and may predispose Suffolk ewes to be more responsive to bacterial infection than Dorset ewes.
Assuntos
Reação de Fase Aguda/veterinária , Comportamento Animal , Regulação da Expressão Gênica/efeitos dos fármacos , Imunidade Inata/efeitos dos fármacos , Lipopolissacarídeos/efeitos adversos , Ovinos/imunologia , Reação de Fase Aguda/induzido quimicamente , Animais , Cruzamento , Citocinas/sangue , Feminino , Hidrocortisona/sangue , Leucócitos/imunologia , Lipopolissacarídeos/imunologia , Ovinos/classificação , Ovinos/fisiologia , Especificidade da EspécieRESUMO
The risk for cardiovascular disease (CVD) increases with advancing age; however, the age at which CVD risk increases significantly is delayed by more than a decade in women compared with men. This cardioprotection, which women experience until menopause, is presumably due to the presence of ovarian hormones, in particular, estrogen. The purpose of this study was to determine how age and ovarian hormones affect flow-induced vasodilation in the coronary resistance vasculature. Coronary arterioles were isolated from young (6 mo), middle-aged (14 mo), and old (24 mo) intact, ovariectomized (OVX), and ovariectomized + estrogen replaced (OVE) female Fischer-344 rats to assess flow-induced vasodilation. Advancing age impaired flow-induced dilation of coronary arterioles (young: 50 +/- 4 vs. old: 34 +/- 6; % relaxation). Ovariectomy reduced flow-induced dilation in arterioles from young females, and estrogen replacement restored vasodilation to flow. In aged females, flow-induced vasodilation of arterioles was unaltered by OVX; however, estrogen replacement improved flow-induced dilation by approximately 160%. The contribution of nitric oxide (NO) to flow-induced dilation, assessed by nitric oxide synthase (NOS) inhibition with N(G)-nitro-l-arginine methyl ester (l-NAME), declined with age. l-NAME did not alter flow-induced vasodilation in arterioles from OVX rats, regardless of age. In contrast, l-NAME reduced flow-induced vasodilation of arterioles from estrogen-replaced rats at all ages. These findings indicate that the age-induced decline of flow-induced, NO-mediated dilation in coronary arterioles of female rats is related, in part, to a loss of ovarian estrogen, and estrogen supplementation can improve flow-induced dilation, even at an advanced age.
Assuntos
Envelhecimento , Circulação Coronária/efeitos dos fármacos , Vasos Coronários/efeitos dos fármacos , Endotélio Vascular/efeitos dos fármacos , Estradiol/administração & dosagem , Terapia de Reposição de Estrogênios , Ovariectomia , Vasodilatação/efeitos dos fármacos , Fatores Etários , Animais , Arteríolas/efeitos dos fármacos , Vasos Coronários/metabolismo , Inibidores de Ciclo-Oxigenase/farmacologia , Implantes de Medicamento , Endotélio Vascular/metabolismo , Inibidores Enzimáticos/farmacologia , Feminino , Hidrazinas/farmacologia , Indometacina/farmacologia , NG-Nitroarginina Metil Éster/farmacologia , Óxido Nítrico/metabolismo , Doadores de Óxido Nítrico/farmacologia , Óxido Nítrico Sintase Tipo III/antagonistas & inibidores , Óxido Nítrico Sintase Tipo III/genética , Óxido Nítrico Sintase Tipo III/metabolismo , Fosforilação , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , RNA Mensageiro/metabolismo , Ratos , Ratos Endogâmicos F344RESUMO
Granulosal cells form highly specialized membrane connections with the oocyte and each other, allowing the passage of regulatory molecules and metabolites between cells. Gene expression changes in granulosal cells may adversely affect oocyte competence resulting in early embryonic loss. The present study was conducted to analyze global gene expression profiles in granulosal cells from persistent ovarian follicles in cows. Cows were assigned randomly to two groups: growing follicles on day 8 and persistent follicles on day 15 of the estrous cycle (estrus=day 0). Cows in the persistent follicle group received progesterone from CIDR-B devices on days 4 through 13. Granulosal cells were collected from both growing and persistent follicles and used in a direct comparison microarray experiment using a bovine long oligo array representing approximately 8400 known genes. Analysis of the microarray data revealed up-regulation of 272 genes (M-value>or=0.9) and down-regulation of 203 genes (M-valueAssuntos
Bovinos/metabolismo
, Células da Granulosa/fisiologia
, Folículo Ovariano/fisiologia
, Animais
, Bovinos/genética
, Feminino
, Perfilação da Expressão Gênica
, Células da Granulosa/metabolismo
, Análise de Sequência com Séries de Oligonucleotídeos/veterinária
, Folículo Ovariano/citologia
, Folículo Ovariano/metabolismo
, RNA Mensageiro/biossíntese
, RNA Mensageiro/genética
, Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária
RESUMO
A high incidence of early embryonic loss is associated with prolonged dominance of follicles. The objective of the present experiment was to determine if persistence of a follicle resulted in alterations in mRNA expression of important genes in the oocyte. Cows were assigned to four groups: growing follicles on day 6 (G0h) or day 8 (G48h) and persistent follicles on day 13 (P0h) or day 15 (P48h) of the estrous cycle (estrus = day 0). All cows were super-stimulated on day 1-4. Cows in G48h, P0h, and P48h groups received 25 mg prostaglandin (PG) F2alpha on day 6. Cows in P0h and P48h groups received progesterone from CIDR-B devices on day 5 through 13. Ovaries of cows in G0h, G48h, P0h, and P48h groups were removed on day 6, 8, 13, and 15, respectively. Oocytes were aspirated immediately after colpotomy and denuded of cumulus cells. Quantitative real-time PCR was used to measure the mRNA abundances of 10 selected genes important for early embryogenesis in oocytes obtained from growing and persistent follicles. Relative abundances of MSY2, PARN, and YY1 mRNA (P < 0.05) were significantly lower in oocytes from persistent than from growing follicles. Oocytes from persistent follicles, however, had greater abundances of PAP and eIF-4E transcripts (P < 0.05). The data indicate that persistence of a follicle leads to altered abundances of mRNA for genes important for regulation of transcription and protein translation in the oocyte, which could compromise development of early embryos in cows that ovulate a persistent follicle.
Assuntos
Estro/metabolismo , Oócitos/metabolismo , Folículo Ovariano/metabolismo , RNA Mensageiro/metabolismo , Transcrição Gênica , Animais , Bovinos , FemininoRESUMO
Initiation of long-term treatment with rbST (Posilac, Monsanto, St. Louis, MO) coincident with first insemination increased pregnancy rates in dairy cattle, but neither the efficacy of using only the initial injection, nor its effects on retention of pregnancy are known. Lactating dairy cows, dairy heifers, and lactating beef cows were assigned at random to treatment (rbST) or control. Dairy cows, dairy heifers, and beef cows received 500 mg rbST (n = 48, 35, 137 inseminations, respectively) at artificial insemination or were left untreated (n = 62, 33, 130 inseminations, respectively). Pregnancy was diagnosed by ultrasonography at 28-36 days. Treatment with rbST at insemination improved conception rates in dairy cows (60.4% versus 40.3%; P < 0.05), but not in dairy heifers or beef cows. Conception rates did not differ in dairy cows at < or =100 days in milk (DIM), but were improved in cows treated with rbST after 100 DIM (64.3% versus 25.8%; P < 0.05). Retention of pregnancy to approximately 60 days and sizes of CL, diameter of follicles > or =5 mm, and crown-rump lengths of embryos were not affected by treatment. The second objective was to examine the effects of rbST at insemination on birth weight and post-natal calf growth in beef cows. However, birth and weaning weights of beef calves were not affected by treatment. In conclusion, a single treatment with rbST at insemination increased conception rates in dairy cows, specifically in those >100 DIM.
Assuntos
Cruzamento/métodos , Bovinos/fisiologia , Fertilização/efeitos dos fármacos , Hormônio do Crescimento/administração & dosagem , Envelhecimento , Animais , Composição Corporal , Corpo Lúteo/anatomia & histologia , Corpo Lúteo/diagnóstico por imagem , Estatura Cabeça-Cóccix , Estradiol/sangue , Feminino , Idade Gestacional , Inseminação Artificial/veterinária , Lactação , Folículo Ovariano/anatomia & histologia , Folículo Ovariano/diagnóstico por imagem , Gravidez , Progesterona/sangue , Proteínas Recombinantes , UltrassonografiaAssuntos
Perda do Embrião/veterinária , Reprodução , Animais , Bovinos , Indústria de Laticínios , Feminino , Carne , GravidezRESUMO
PROBLEM: Spontaneous mastitis or induced infections mimicking mastitis reduce pregnancy rates in ruminants. The effect of immunization with either a mastitis-related pathogen component, peptidoglycan-polysaccharide (PG-PS), or killed Streptococcus pyogenes on pregnancy outcome was investigated. METHOD OF STUDY: Ewe lambs were immunized with PG-PS (n = 50) or killed bacteria (n = 50) or were not immunized (control, n = 100). Titers of PG-PS immunoglobulin G (IgG) were detected by enzyme-linked immunosorbent assay (ELISA). Ewes were bred by rams at synchronized estrus. All immunized ewes and half of the ewes not immunized were challenged with PG-PS on day 5 after breeding. Pregnancy maintenance was evaluated. RESULTS: Although the proportion of ewes pregnant at day 42 after breeding did not differ among treatments, the probability of pregnancy decreased with total dose of PG-PS (P < 0.05). CONCLUSIONS: Immunization of ewe lambs with PG-PS or killed S. pyogenes did not improve pregnancy maintenance. Furthermore, the toxic streptococcal component decreased pregnancy rate in immunized sheep in a dose-dependent manner.
Assuntos
Peptidoglicano/imunologia , Polissacarídeos/imunologia , Prenhez/imunologia , Reprodução/imunologia , Reprodução/fisiologia , Ovinos/fisiologia , Animais , Feminino , Imunização , Gravidez , Resultado da Gravidez , Prenhez/fisiologiaRESUMO
Potential factors affecting retention of pregnancy during weeks 5-9 of gestation were studied in dairy cows and heifers (N = 211) on two farms. Cows were examined by ultrasonography for presence of a viable embryo, and sizes of the corpus luteum (CL) and of follicles > or = 5mm were recorded. Blood samples were taken at each examination and assayed for progesterone and estradiol. Overall pregnancy loss was 11.4%. Cows with two CL did not have greater concentrations of progesterone than cows with one CL and they retained fewer pregnancies (P < 0.01; 73% versus 91%). Pregnancy retention was associated positively with concentrations of progesterone and estradiol during week 5 (P < 0.05). Embryos that were lost apparently died before CL regression. Retention of pregnancy declined in cows with high body condition and as age of the cow increased. Pregnancy retention was lower in cows bred to one of four frequently-used service sires (P < 0.05). Days postpartum, milk production, parity, service number, inseminator, synchronization of estrus, diameter of follicles and size of CL did not affect pregnancy retention. In conclusion, retention of pregnancy during placentation varied with concentrations of progesterone and estradiol, age of cow, body condition and service sire.