[The cytogenetic analysis of unfertilized human oocytes]. / Tsitogeneticheskii analiz neoplodotvorennykh ootsitov cheloveka.

Cytogenetic analysis of 186 unfertilized human oocytes obtained from 145 women were carried out according to programs of in vitro fertilization. The ratio of different cytogenetic types of oocytes in their heterogenic population was determined. At least 30% of oocytes were shown to have nuclear and cytoplasmic immaturity. The level of chromosome anomalies in oocytes of second meiotic metaphase comprised 30.8%.

Evaluation of immunofluorescence on pig zone pellucida for detection of anti-zone antibodies in human sera.

Using immunofluorescence tests, antibodies reacting with pig zone pellucida could be detected in 31% of 103 sera from patients in different clinically defined categories, mainly fertile males and females and patients from couples with unexplained infertility. The antibodies could in all cases be absorbed by means of pig erythrocytes - in most sera with all erythrocyte suspensions tested, but in some cases only with erythrocytes from certain animals - indicating that antibodies both to generally occurring pig antigens and alloantigens may be present in human sera. Staining of pig zonae was recorded equally frequently with unabsorbed sera from infertile and fertile males and females, respectively, but titres of 1:16 or more occurred more frequently among infertile than among fertile female (P = 0.053).