RESUMO
Gastropod-associated nematodes have been previously studied and documented worldwide, with some species forming host-specific association as obligate parasites of molluscs while others form intermediate and temporary association. Philippinella moellendorffi from Imelda, Zamboanga Sibugay, Philippines, were collected, washed and maintained in the laboratory until death. Cadavers were placed on nutrient agar to allow nematode proliferation. Nematode pure culture was obtained using one gravid female for propagation. Morphology and molecular analyses (18S ribosomal DNA (rDNA) and D2-D3 expansion segments of 28S rDNA) were employed as diagnostic tools in identifying the nematode species isolated from P. moellendorffi. The newly isolated nematode was identified as Caenorhabditis brenneri, thus designated as C. brenneri strain IZSP from the Philippines. This is the first record of C. brenneri isolated from the terrestrial slug P. moellendorffi.
Assuntos
Caenorhabditis , Gastrópodes , Nematoides , Rabditídios , Animais , Cadáver , Caenorhabditis/genética , DNA de Helmintos/genética , DNA Ribossômico/genética , Feminino , Gastrópodes/parasitologia , Filipinas , Filogenia , Rabditídios/anatomia & histologiaRESUMO
Vinte pacientes com IAo foram tratados conservadoramente através de plástica dos folhetos. Os defeitos concomitantes foram corrigidos. Todos apresentavam insuficiência cardíaca que apresentou melhora após a cirurgia. Conclui-se que: 1) a correçäo conservadora da IAo é eficaz, sendo opçäo válida. 2) no período de estudo - de 3 a 5 anos - näo houve desapeptaçäo da funçäo do VE apesar de permanecerem sinais auscultatórios de IAo