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J Food Prot ; 73(6): 1129-37, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20537273

RESUMO

The present study was aimed at characterizing the ability of lactic acid bacteria isolated from maize to repress the growth of fumonisin-producing fungi. A total of 67 isolates were screened for their antifungal activity against Fusarium proliferatum and Fusarium verticillioides by using the overlay method. The most efficient antifungal isolate was identified as Pediococcus pentosaceus (L006), on the basis of physiological and biochemical characterization and 16S rRNA gene sequencing. Production of the antifungal metabolite by this isolate commenced at the end of the growth exponential phase (8 h) and reached a maximum level after a long period of incubation (120 h). The antifungal metabolites produced were shown to be heat stable, resistant to proteolytic enzyme treatments, and pH dependent. The exact chemical nature of these substances remains to be clarified.


Assuntos
Fumonisinas/metabolismo , Fusarium/crescimento & desenvolvimento , Fusarium/metabolismo , Pediococcus/fisiologia , Zea mays/microbiologia , Antibiose , Qualidade de Produtos para o Consumidor , Contaminação de Alimentos/análise , Contaminação de Alimentos/prevenção & controle , Microbiologia de Alimentos , Conservação de Alimentos/métodos , Concentração de Íons de Hidrogênio , Cinética , Folhas de Planta , RNA Fúngico/análise , RNA Ribossômico 16S/química , RNA Ribossômico 16S/genética , Zea mays/química
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