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1.
Rev Fac Cien Med Univ Nac Cordoba ; 79(3): 289-293, 2022 09 16.
Artigo em Espanhol | MEDLINE | ID: mdl-36149066

RESUMO

Introduction: Transosseous biopsy allows sampling of lesions that are difficult to access with conventional techniques. Its use avoids surgeries. Objective: To present a clinical case in which retroperitoneal percutaneous biopsy with trans vertebral approach was used. A brief bibliographic revision of this technique will be made. Case: 60 year old woman with endometrial adenocarcinoma, with 7 months of clinical symptoms characterized by asthenia and non-specific lumbar pain. An intercaval aortic lymphadenopathy was found. Trans vertebral biopsy of the lesion was decided, its location precluded conventional approaches access. Conclusion: This technique must be considered when studying unreacheable lesions by other means and performed by trained professionals.


Introducción: La biopsia trans ósea permite el estudio de lesiones que presentan accesos convencionales bloqueados por otras estructuras, como órganos vitales. Su uso evita procedimientos de mayor complejidad. Objetivo: Reportar un caso clínico en el cual se utilizó la técnica de biopsia percutánea con abordaje trans vertebral para toma de muestra. En forma secundaria se hará una breve revisión de la bibliografía. Caso: Caso: Mujer de 60 años, con adenocarcinoma de endometrio con cuadro clínico de 7 meses caracterizado por astenia y dolor lumbar. Presentaba una linfadenopatía intercavo-aórtica. Se decidió biopsiar de forma trans vertebral debido a que su ubicación limitaba otros abordajes. Conclusión: Este abordaje debe ser considerado para acceder a lesiones inalcanzables por otras vías y ser empleado por profesionales entrenados.  Palabras claves: biopsia guiada por imágenes; metástasis linfática; neoplasias de endometrio.


Assuntos
Linfadenopatia , Tomografia Computadorizada por Raios X , Feminino , Humanos , Biópsia Guiada por Imagem/métodos , Linfadenopatia/diagnóstico por imagem , Linfadenopatia/patologia , Pessoa de Meia-Idade , Espaço Retroperitoneal , Tomografia Computadorizada por Raios X/métodos
2.
Clin Exp Metastasis ; 30(8): 993-1007, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23832740

RESUMO

Lung cancer is the most frequent and one of the most deadly cancer types and is classified into small cell lung cancer and non-small cell lung cancer (NSCLC). Transforming growth factor beta (TGFß) regulates a wide array of cell functions and plays a major role in lung diseases, including NSCLC. TGFß signals through the complex of TGFß type I and type II receptors, triggering Smad and non-Smad signaling pathways such as PI3K/Akt and MEK1/ERK. We investigated the role of TGFß1 on the progression of the murine lung adenocarcinoma cell line LP07. Furthermore, we undertook a retrospective study with tissue samples from stage I and II NSCLC patients to assess the clinical pathologic role and prognostic significance of TßRI expression. We demonstrated that although lung cancer cell monolayers responded to TGFß1 anti-mitogenic effects and TGFß1 pulse (24 h treatment) delayed tumor growth at primary site; a switch towards malignant progression upon TGFß1 treatment was observed at the metastatic site. In our model, TGFß1 modulated in vitro clonogenicity, protected against stress-induced apoptosis and increased adhesion, spreading, lung retention and metastatic outgrowth. PI3K and MEK1 signaling pathways were involved in TGFß1-mediated metastasis stimulation. Several of these TGFß responses were also observed in human NSCLC cell lines. In addition, we found that a higher expression of TßRI in human lung tumors is associated with poor patient's overall survival by univariate analysis, while multivariate analysis did not reach statistical significance. Although additional detailed analysis of the endogenous signaling in vivo and in vitro is needed, these studies may provide novel molecular targets for the treatment of lung cancer.


Assuntos
Adenocarcinoma/secundário , Carcinoma Pulmonar de Células não Pequenas/patologia , Carcinoma de Células Escamosas/secundário , Neoplasias Pulmonares/patologia , Proteínas Serina-Treonina Quinases/metabolismo , Receptores de Fatores de Crescimento Transformadores beta/metabolismo , Fator de Crescimento Transformador beta/metabolismo , Adenocarcinoma/metabolismo , Adenocarcinoma/mortalidade , Idoso , Idoso de 80 Anos ou mais , Animais , Apoptose , Western Blotting , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Carcinoma Pulmonar de Células não Pequenas/mortalidade , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/mortalidade , Adesão Celular , Ciclo Celular , Movimento Celular , Proliferação de Células , Transição Epitelial-Mesenquimal , Feminino , Imunofluorescência , Seguimentos , Humanos , Técnicas Imunoenzimáticas , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/mortalidade , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Fosfatidilinositol 3-Quinases/metabolismo , Prognóstico , Proteínas Proto-Oncogênicas c-akt/metabolismo , Receptor do Fator de Crescimento Transformador beta Tipo I , Estudos Retrospectivos , Taxa de Sobrevida
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