Effect of enriched environment and predictable chronic stress on spatial memory in adolescent rats: Predominant expression of BDNF, nNOS, and interestingly malondialdehyde in the right hippocampus.

Little is known about the mechanisms that promote divergence of function between left and right in the hippocampus, which is most affected by external factors and critical for spatial memory. We investigated the levels of memory-related mediators in the left and right hippocampus and spatial memory in rats exposed to predictable chronic stress (PCS) and an enriched environment (EE) during adolescence. Twenty-eight-day-old Sprague-Dawley rats were divided into control (standard cages), PCS (15 min/day immobilization stress for four weeks), and EE (one hour/day environmentally enriched cages for four weeks) groups. After the applications, spatial memory was tested with the Morris water maze, and the serum levels of corticosterone were evaluated. The levels of brain-derived neurotrophic factor (BDNF) and neuronal nitric oxide synthase (nNOS), which are critical for synaptic plasticity; malondialdehyde (MDA; lipid-peroxidation indicator); protein carbonyl (protein-oxidation indicator); and superoxide dismutase (antioxidant enzyme) were evaluated in the left and right hippocampus. Corticosterone levels in both the PCS and EE groups did not change compared with control. In both the PCS and EE groups, spatial memory improved and BDNF was increased in both halves of the hippocampus, still there was an asymmetry. nNOS levels were increased in the dentate gyrus and CA1 regions of the right hippocampus in both PCS and EE groups. MDA levels were increased but PCO levels were decreased in the right hippocampus in both the PCS and EE groups, but SOD did not change in either half of the hippocampus. Our results suggest that both PCS and EE improved spatial memory by increasing BDNF and nNOS in the right hippocampus and that, interestingly; MDA could be the physiological signal molecule in the right hippocampus for spatial memory process.

Localization of the aromatase enzyme expression in the human pituitary gland and its effect on growth hormone, prolactin, and thyroid stimulating hormone axis.

The aim of this study is to evaluate aromatase expression in prolactin (PRL), thyroid stimulating hormone (TSH), and growth hormone (GH) secreting cells. Nontumoral human pituitary specimens were obtained from autopsy samples. Aromatase co-expression was determined by double immunohistochemical staining and assessed using H scores. H scores for GH-aromatase co-expression (GH-aromatase), TSH-aromatase co-expression (TSH-aromatase), and PRL-aromatase co-expression (PRL-aromatase) were 83.1 ± 13.1, 95.6 ± 16.1, and 83.7 ± 14.5, respectively. TSH producing cells exhibited the highest H score for co-expression of aromatase (p < 0.001). There was no gender difference in terms of H scores for aromatase expression and double immunohistochemical staining results (p > 0.05 for all). There was a negative correlation between the H scores for aromatase and PRL-aromatase, GH-aromatase and TSH-aromatase, respectively (r = -0.592, p < 0.001; r = -0.593, p < 0.001; r = -0.650, p < 0.001, respectively). Also, H scores for aromatase co-expression of each hormone were negatively correlated with the H scores for the corresponding hormone (r = -0.503, p < 0.001 for PRL-aromatase and PRL; r = -0.470, p < 0.001 for GH-aromatase, and GH; r = -0.641, p < 0.001 for TSH-aromatase and TSH). H scores for mean aromatase, GH-aromatase, TSH-aromatase were invariant of age (p > 0.05 for all). Age was negatively correlated with PRL-aromatase H score (r = -0.373, p = 0.008). Our study demonstrated significant aromatase co-expression in PRL, GH, and TSH secreting cells of the human anterior pituitary gland. The mutual paracrinal regulation between aromatase and three adenohypophyseal hormones indicates that aromatase may have a regulatory role on the synthesis and secretion of these hormones.

Aromatase cytochrome P450 enzyme expression in prolactinomas and its relationship to tumor behavior.

The aim of the study was to evaluate the presence of aromatase cytochrome P450 enzyme (P450AROM) expression in normal pituitary tissues and tumor tissues of patients with prolactinoma and to examine the impact of the P450AROM expression on clinical outcome. Twenty-six consecutive human pituitary tissue samples were obtained from autopsies performed at the Institute of Forensic Medicine. Sixty-four patients who had an adenomectomy between 2000 and 2009 after prolactinoma diagnosis with histologically confirmed pituitary tumor tissues were retrospectively included in this study. The slices from the pituitary tissues were subjected to immunohistochemical staining for evaluation of P450AROM and estrogen receptor beta (ER beta) subunit. Immunohistochemistry results were compared according to age, gender, remission rate, resistance and invasion status of the patients. Higher than normal P450AROM expression was found in the pituitary tissues of the patients with prolactinoma (p < 0.001). P450AROM intensity had no relation to resistance or remission in patients with prolactinoma (p = 0.44, p = 0.45, respectively). The subgroup analysis showed that compared to males without invasive adenoma, males with invasive adenoma had higher P450AROM expression (p = 0.048). ER beta was found to have an impact on resistance (p = 0.049). This study shows that P450AROM expression is present in the pituitary tissues of patients with prolactinoma and that this presence could be important in development and tumor behavior of prolactinomas.

Cell growth on in situ photo-cross-linked electrospun acrylated cellulose acetate butyrate.

In this study, electrospinning was combined with UV curing technology for producing in situ photo cross-linked fibers from methacrylated cellulose acetate butyrate (CABIEM). ECV304 and 3T3 cells were seeded on electrospun fibrous scaffolds. Collagen modified CABIEM fibers were also prepared for improving cell adhesion and proliferation. Cross-linking and the morphology of the fibers were characterized by ATR-FTIR spectrometry and environmental scanning electron microscopy (ESEM). The cytotoxicity of the fibers was examined using the MTT cytotoxicity assay. According to the results, electrospun fibrous scaffolds are non-toxic and cell viability depends on the amount of collagen. It was found that cell adhesion and cell growth were enhanced as the collagen percentage was increased.

Effects of Luteolin on Liver, Kidney and Brain in Pentylentetrazol-Induced Seizures: Involvement of Metalloproteinases and NOS Activities.

OBJECTIVE: Flavonoids are an important group of recognized antioxidants in plants. Luteolin (LUT) is a natural flavonoid in the plant kingdom. This study was aimed to investigate the effects of the LUT in the liver, kidney and brain of pentylentetrazol (PTZ)-induced seizure and the relationship between nitric oxide synthases (iNOS, eNOS) and matrix metalloproteinases (MMP2, MMP9). MATERIALS AND METHODS: LUT (10 mg/kg) was given intraperitoneally during two weeks prior to seizure induction. A single dose PTZ 80 mg/kg i.p. was administered and seizures were observed and evaluated with regard to latency, frequency and stage for one hour. RESULTS: Seizure frequen cy after PTZ administration was significantly decreased in LUT pretreated rats (p<0.05). An increase of immunhistochemical reactions of iNOS and MMP2, but a decrease of eNOS activity, were observed in rat hippocampus and peripheral tissues during the PTZ induced seizures. LUT pretreatment reversed the iNOS and MMP2 activity to the control levels and significantly increased the eNOS activity (p<0.001). CONCLUSION: LUT seems to have an effective role in reducing the seizure frequency and a protective role on peripheral organ injury in animal models of seizure. The protective effect of LUT in seizures and the seizure induced peripheral tissue damage warrant further investigations.