Ultrasonographic kidney length-to-abdominal aortic diameter for the diagnosis of feline chronic kidney disease: A preliminary study.

Background and Aim: Chronic kidney disease (CKD) is one of the most important diseases in cats. This study aimed to compare the ultrasonographic kidney length-to-abdominal aortic diameter (K/AO) ratio between healthy and CKD cats and investigate the correlation between K/AO and blood results. Materials and Methods: Fifteen healthy cats and 15 CKD cats were included in this clinically prospective study. All cats were evaluated for radiographic and ultrasonographic K, radiographic K-to-second lumbar length ratio (K/L2), and K/AO, indirect systolic blood pressure and plasma creatinine (Cr), blood urea nitrogen (BUN), and symmetric dimethyl arginine (SDMA). Results: The radiographic and ultrasonographic kidney lengths of CKD were significantly shorter than those of healthy cats (p < 0.05 and p < 0.05, respectively). The average K/L2 and K/AO were significantly lower in CKD than in healthy cats (p < 0.01 and p < 0.001, respectively). The K/AO had a strong negative correlation with plasma Cr (r = -0.7682, p < 0.0001), BUN (r = -0.6175, p < 0.001), and SDMA (r = -0.589, p < 0.001). However, K/L2 had a moderate negative correlation with plasma Cr (r = -0.5866, p < 0.001), BUN (r = -0.4884, p < 0.01), and SDMA (r = -0.5404, p < 0.01). The optimal cutoff value of K/AO (<10.71) had higher sensitivity and specificity than K/L2 for identifying feline CKD. Conclusion: Kidney length-to-abdominal aortic diameter could be a better and more promising parameter than the K/L2 ratio for evaluating kidney size in cats with CKD.

The Feasibility of Ultrasonographic Diaphragmatic Excursion in Healthy Dogs: Effect of Positioning, Diaphragmatic Location, and Body Weight of Dogs.

Diaphragmatic excursion (DE) has been utilized for detecting respiratory related problems in humans. However, several factors should be considered such as the ultrasound technique and factors intrinsic to patients. Nevertheless, knowledge of the effect of these factors on DE in dogs is still lacking. The aim of this study was to evaluate the proper ultrasound technique by varying postures and diaphragmatic locations for DE measurement and to explore intrinsic factors such as diaphragmatic sides, sex, and body weight of dogs on DE. The prospective, analytic, cross-sectional study included 44 healthy dogs; 12 beagles and 32 dogs of other breeds. The experiment was divided into (i) an exploration of the proper ultrasound technique by varying postures (supine, standing, and recumbent in each of the right and left lateral positions), diaphragmatic locations (middle crus and proximal to the last rib), and diaphragmatic sublocations (xiphoid, mid, and proximal rib) for detection of DE and (ii) the evaluation of canine intrinsic factors affecting DE. The results show that the mid-diaphragmatic sublocation in the middle crus area in almost all positions revealed the highest percentage DE detection. However, DEs were revealed to be more accessible in the supine position. There was no significant difference in DE between the right and the left diaphragms or between the sexes of beagle dogs. However, body weight was significantly correlated with the DE among dogs of various sizes. In conclusion, the posture of the dogs and the diaphragmatic location can affect DE evaluation. Neither sex nor diaphragmatic side had an influence, but body weight was revealed as a major factor in DE in dogs.

Comparison of radiographic and computed tomographic acetabular index in small-breed dogs: a preliminary study using Maltese and Shih Tzu.

BACKGROUND: The morphometry of the acetabulum is one source of information that assists in the clinical diagnosis of the hip and influences the proper selection of a prosthesis, reducing post-operative complications such as those seen in total hip replacement (THR). However, acetabular parameters in small-breed dogs are rarely reported. OBJECTIVES: To investigate acetabular parameters using radiography and computed tomography (CT) in small-breed dogs with Maltese and Shih Tzu dogs used as model breeds. METHODS: Standard calibrated, extended hip radiographs and CT images were obtained. Subsequently, acetabular width (AW) in various directions was measured using radiography and CT, whereas acetabular depth (AD) was obtained by CT. Acetabular index (AI) is a ratio calculated from AD and AW. RESULTS: The values of AW and AD were much higher in Shih Tzu than in Maltese dogs. Male Shih Tzus showed higher values of these parameters than females, while sex-based differences in most of the parameters could not be detected in Maltese. Body weight, but not age, influenced AWs and ADs. While AWs and ADs were influenced by several factors, AI was comparable among the assessed factors and between Maltese and Shih Tzu dogs (p = 0.172; 31.42 ± 1.35 and 32.60 ± 1.80, respectively). Also, AI did not vary with breed, sex, or body size. CONCLUSIONS: The obtained radiographic and CT acetabular parameters could be useful as guidelines for evaluating the acetabulum of small-breed dogs in clinical practice.

Comparison of computed tomographic ocular biometry in brachycephalic and non-brachycephalic cats.

BACKGROUND AND AIM: Ocular biometry has been used to evaluate ocular parameters; however, several factors need to be considered. In humans, age and sex have been shown to affect ocular biometry. The main factor that affects feline ocular biometry is the head circumference. At present, several reports have revealed that canine ocular biometry differs among dog breeds. However, there are no reports on normal ocular biometry in cats using computed tomography (CT). Therefore, this study aimed to explore feline ocular parameters between brachycephalic (B) and non-brachycephalic (NB) cats using CT and to evaluate the influence of age or sex of cats on ocular biometry. MATERIALS AND METHODS: Twenty-four normal cats were divided into two groups: B (n=12) and NB (n=12). Each group had an equal number of designated males and females. CT was performed under mechanical restraint without general anesthesia and intravenous contrast enhancement. Ocular biometry, dimensions of the internal structure, including attenuation numbers and extra-ocular structures, were evaluated and compared. RESULTS: B-cats had a significantly wider globe width (GW) than NB-cats (p<0.05). In addition, globe length (GL) and GW were significantly correlated with the age of the cats. Significant correlation between GL and age was observed in all cats (r=0.4867; p<0.05), NB-cats (r=0.8692; p<0.05), and B-cats (r=0.4367; p<0.05), whereas the correlation between GW and age was observed in B-cats only (r=0.7251; p<0.05). For extra-ocular structures, NB-cats had significantly greater orbital depth than B-cats (p<0.05), and orbital diameter was significantly correlated with age in all cats and B-cats (p<0.05). CONCLUSION: CT can be used for ocular biometric evaluation in cats with different skull types. GW was wider in B-cats, whereas the orbital depth was greater in NB-cats. Moreover, GW, GL, and orbital diameter were affected by the age of the cats. This information will be useful for further ocular diagnosis and treatment, especially in prosthetic surgical procedures.

Multi-factorial considerations for intra-thoracic lymph node evaluations of healthy cats on computed tomographic images.

BACKGROUND: It is difficult to examine mild to moderate feline intra-thoracic lymphadenopathy via and thoracic radiography. Despite previous information from computed tomographic (CT) images of intra-thoracic lymph nodes, some factors from animals and CT setting were less elucidated. Therefore, this study aimed to investigate the effect of internal factors from animals and external factors from the CT procedure on the feasibility to detect the intra-thoracic lymph nodes. Twenty-four, client-owned, clinically healthy cats were categorized into three groups according to age. They underwent pre- and post-contrast enhanced CT for whole thorax followed by inter-group evaluation and comparison of sternal, cranial mediastinal, and tracheobronchial lymph nodes. RESULTS: Post contrast-enhanced CT appearances revealed that intra-thoracic lymph nodes of kittens were invisible, whereas the sternal, cranial mediastinal, and tracheobronchial nodes of cats aged over 7 months old were detected (6/24, 9/24 and 7/24, respectively). Maximum width of these lymph nodes were 3.93 ± 0.74 mm, 4.02 ± 0.65 mm, and 3.51 ± 0.62 mm, respectively. By age, lymph node sizes of these cats were not significantly different. Transverse lymph node width of males was larger than that of females (P = 0.0425). Besides, the detection score of lymph nodes was affected by slice thickness (P < 0.01) and lymph node width (P = 0.0049). Furthermore, an irregular, soft tissue structure, possibly the thymus, was detected in all juvenile cats and three mature cats. CONCLUSIONS: Despite additional information on intra-thoracic lymph nodes in CT images, which can be used to investigate lymphatic-related abnormalities, age, sex, and slice thickness of CT images must be also considered.

Computed tomographic appearance of circumcaval and circumuterine ureter in a cat.

BACKGROUND: Circumcaval ureter is a rare congenital anomaly resulting from the persistence of embryonic venous system. This anomaly is characterized by running of affected ureter around caudal vena cava (CVC). CASE PRESENTATION: In this report, circumcaval ureter was found in a 2-year-old female cat attending as normal sample group in another research. This cat passed all health checkup protocols before computed tomography (CT) was performed. CONCLUSION: The contrast-enhanced computed tomographic (cCT) images clearly revealed the dislocation of the right ureter that course around CVC and uterine body. This is the first report of feline circumcaval concurrent with circumuterine ureter detected by cCT.

Morphological assessment of cat kidneys using computed tomography.

There are numerous publications about feline renal imaging information; however, none have established reference values for kidney size using computed tomography (CT). This study aimed to determine renal size and shape as well as the morphology of renal-related structures in clinically normal cats (Felis catus) that underwent CT. Twenty-seven healthy cats underwent pre- and post-iodinated contrast-enhanced CT. Most cat (59%) kidneys were located at the same level. The average pre-contrast dimensions of the left kidney included a width of 2.46 ± 0.28 cm, a length of 3.52 ± 0.44 cm and a height 2.19 ± 0.31 cm, whereas those of the right kidneys were 2.45 ± 0.27 cm, 3.54 ± 0.46 cm and 2.05 ± 0.23 cm, respectively. After contrast enhancement, kidneys were slightly enlarged though not significantly. Additionally, renal length (LK or RK) was compared with second lumbar vertebra (L2) length and abdominal aorta diameter (AO). AO was significantly larger in male cats whereas L2 length appeared longer in male cats, but was not statistically different from the female cats. The LK/L2 and RK/L2 ratios were 2.29 ± 0.23 and 2.36 ± 0.20, respectively, and the LK/AO and RK/AO were 11.72 ± 1.37 and 12.05 ± 1.47, respectively. Renal vessels were examined. The renal vein was obviously larger than the renal artery, and paired renal veins were observed periodically. This study provides CT information about the feline kidney, which may help to establish reference values and information regarding renal structure prior to surgery in practice.

Identification of two distinct types of beta-2 microglobulin in marine fish, Pagrus major and Seriola quinqueradiata.

Two types of beta-2 microglobulin (beta2m) were identified in red seabream and yellowtail from expressed sequence tag (EST) analyses of kidney and spleen cells. A cluster of 21 ESTs from red seabream showed high homology to beta2m from other teleosts, while another cluster containing 7 ESTs was also homologous to beta2m. Similarly, two clusters containing 21 and 2 ESTs were identified to be homologous to beta2m in yellowtail. The genes in the large and small clusters of each fish were designated beta2m-1 and beta2m-2, respectively. Although the amino acid sequences of beta2m-1s were highly homologous to those identified in the other teleosts, those of beta2m-2s were less homologous to fish beta2m. beta2m-1 of red seabream lacks the first intron of two introns observed in vertebrates, and beta2m-2 lacks both introns. Phylogenetic analysis of vertebrate beta2m proteins suggested that the beta2m-2 in marine fish are distinct from beta2ms in the other teleosts.

Immune-related gene expression profiling of yellowtail (Seriola quinqueradiata) kidney cells stimulated with ConA and LPS using microarray analysis.

To better understand the immune system of a commercially important fish (yellowtail, Seriola quinqueradiata), we constructed a cDNA microarray containing 1001 selected genes from yellowtail EST and used this to investigate gene expression of primary cultured kidney cells stimulated with ConA and LPS. The total number of up-regulated genes stimulated by LPS was apparently greater than that of ConA stimulation, whereas down-regulated genes were markedly found in ConA-stimulated group. Of the genes that were up-regulated at 3, 6, and 12h after LPS treatment, 12%, 13% and 12%, respectively, were immune-related. Immune-related genes were sorted into 4 groups based on their differential expression patterns against LPS induction. LPS induced the expression of genes related to inflammation, cytokine activity, antigen presentation and antigen binding such as, IL-1beta, CC chemokine with stalk CK2, MHC class II beta chain and immunoglobulin heavy chain. Amplified fragments of RT-PCR products of IgM, IL-1beta, nephrosin, and beta-actin had signal intensities that were comparable to those obtained with the microarray. Overall, these results show that microarrays are a promising tool for uncovering immune mechanism in teleost fish. cDNA sequences of genes were deposited in the GenBank database at DDBJ with accession numbers BB 996897-BB 997897.