[The effect of antihypertensive preparations on carbohydrate metabolism in intact rats and in alloxan diabetes]. / Vliianie nekotorykh antigipertenzivnykh preparatov na uglevodnyi obmen intaktnykh krys i pri alloksanovom diabete.

The metabolic affects of anapriline, captopril, and clophelin were studied in the blood and liver homogenates ex vivo and in vitro in experiments on intact rats and rats with alloxan diabetes. Anapriline lowered the blood lactate content in rats with diabetes and intact rats, reduced the level of glycemia in animals with marked hyperglycemia. Captopril increased the blood insulin and lactate content in rats with diabetes and the content of pyruvate in vivo. The results of the study show that the use of clophelin and anaprilin in the treatment of patients with diabetes mellitus is most rational.

[Morphofunctional characteristics of the testis of albino rats exposed to dioxin-containing herbicide 2,4-D]. / Morfofunktsional'naia kharakteristika semennikov belykh krys pri vozdeistvii dioksinsoderzhashchego gerbitsida 2,4-D.

Endocrine system of the gonads of white rats after subacute poisoning with 2,4-D was studied. Signs of increased steroidogenesis in Leydig cells, a decrease of plasma testosterone concentration and decrease of the gonad sensitivity to chorionic gonadotropin were observed.

[The effect of the dioxin-containing herbicide 2,4-D on the hormonal status of experimental animals]. / Vliianie dioksinsoderzhashchego gerbitsida 2,4-D na gormonal'nyi status éksperimental'nykh zhivotnykh.

The article presents studies of hormonal state in rats under daily or 20-day peroral administration of variable doses of 2,4-D herbicide containing dioxine. Changed levels of thyroid hormones, insulin, cortisol, testosterone and estradiol appeared to be divergent and dose-dependent, proving direct toxic effects of the herbicide in endocrine organs, altered hormonal effects in target organs and disorders of peripheral hormonal metabolism.

[Antioxidant protection and endocrine gland function in children in a petroleum industrial center]. / Antioksidantnaia zashchita i funktsiia zhelez vnutrennei sekretsii u detei v nefteindustrial'nom tsentre.

The article presents data on influence of technogenic environmental pollution and parents' occupational hazards on sex-matched physical development of preschoolers, their endocrine balance and antioxidant system. The identified features of physical development, endocrine state and antioxidant system prove higher sensitivity of girls to chemical hazards. Significance of the studied parameters necessitate their use in biologic and ecologic monitoring.

[Isoprotein composition of alpha 1-antitrypsin, haptoglobin, transferrin, and lipid peroxidation in the blood in inflammatory lung diseases in children]. / Izobelkovyi sosotav alpha 1-antitripsina, gaptoglobina, transferrina iperekisnoe okislenie lipidov krovi pri vospalitel'nykh zabolevaniiakh legkikh u detei.

Relationship was studied between the polymorphism of alpha 1-antitrypsin (Pi), haptoglobin (Hp) and transferrin and the development of chronic unspecific impairment of children lungs. Combination of protein isoforms PiM3M3 and Hp 1-1 was found to increase the risk of the lungs unspecific impairments in persons with lingering pneumonia and caused more severe development of the disease. Relatively low activity of the isoform PiM3M3, as compared with other forms of the PiMM system, and more intensive and nonadequate rate of lipid peroxidation in patients containing HP 1-1 appear to be responsible for aggravation of these impairments.

[Production of recombinant proteins and their use in immunoblotting]. / Poluchenie rekombinantnykh belkov i ikh ispol'zovanie v metode immunoblotta.

Chemico-enzymatic synthesis and cloning of genes coding viral proteins--precursors of p50 and gp120 in the eukaryotic vector system were carried out. The recombinant proteins obtained were shown to be used in immunochemical evaluation of AIDS virus antibodies in blood serum.

[Changes in the testosterone levels in the serum and androgen receptor levels in the liver during experimental burns in immature rats]. / Izmeneniia soderzhaniia testosterona v syvorotke krovi i retseptorov androgenov v pecheni pri éksperimental'noi ozhogovoi bolezni u nepolovozrelykh krys.

Distinct and stable decrease of testosterone content was found in blood serum of one month old rats with burns of the IIIA-IIIB degree impairing 23-25% of body surface. Concentration of androgene receptors was markedly decreased in liver cytosol of the experimental animals within 1 day and to the end of the first week after the thermic trauma. Possible mechanisms involved in impairment of androgene-dependent regulation of metabolism under conditions of thermic burns are discussed.

[Radioreceptor method of determination of opioid peptides in the rat brain]. / Radioretseptornyi metod opredeleniia opioidnykh peptidov v golovnom mozge krys.

A procedure is described for quantitative radioreceptor estimation of opioid peptides in rat brain using lyophilized membrane preparations. As opiate receptors exhibit high rate of heterogeneity estimation of delta- and mu-opioid activities was carried out by means of displacing of 3H-DADLE and 3H-DAGO, respectively. Content of opioid peptides in brain of preadolescent rats was studied during experimental burn trauma using the procedure developed. An increase in the delta- and mu-opioid activities was found in rat brain tissue within one day after thermal burns.

[Changes in the cyclic nucleotide system during experimental burns in immature rats]. / Izmeneniia v sisteme tsiklicheskikh nukleotidov pri éksperimental'noi ozhogovoi bolezni u nepolovozrelykh krys.

Thermal trauma caused a distinct increase in content of cAMP and cGMP in blood as well as in liver and kidney tissues of one-month-old rats within the first week after burns. Alterations, which depend on the step of burn development, were found in basal and adrenaline, fluoride-stimulated activities of adenylate cyclase in liver and kidney tissues of experimental animals. Activity of phosphodiesterase was altered only slightly under similar experimental conditions while pronounced decrease in the enzymatic activity was observed in rat liver tissue within 6 hrs after burns.

[Effect of retabolyl and oxymetacyl on protein metabolism in experimental burns in immature rats]. / Vliianie retabolila i oksimetatsila na belkovyi obmen pri éksperimental'noi ozhogovoi bolezni u nepolovozrelykh krys.

Impairments of protein metabolism, expressed as predominance of catabolism over anabolism and hypoproteinemia, were observed in one month old rats with experimental burns of the IIIA-IIIB degree, when 20-25% of a body surface was impaired. After administration of steroid drugs (retabolyl at a dose of 1 mg/100 g of body mass or oxymetacyl--4-methyl-5-oxyuracil---at a dose of 5 mg/100 g of body mass) content of protein and urea normalized in blood serum, activity of cathepsin D decreased in tissues, the rate of 35S-methionine incorporation into tissue proteins increased. The pyrimidine derivative oxymetacyl exhibited higher effect on protein metabolism in burns of preadolescent rats as compared with retabolyl.