RESUMO
AIM: Study adhesive activity of enterobacteria in the model of hemagglutination reaction with animal and avian erythrocytes and clarify structures responsible for adhesion in enterobacteria. MATERIALS AND METHODS: 58 cultures of enterobacteria were used, of which: 21 Escherichia coli strains, 13 Citrobacter spp., 11 Morganella morganii, 9 Proteus spp., 4 Hafnia alvei. Erythrocytes of various animals and birds were used in hemagglutination reaction. Electron-microscopical studies were carried out in JEM-100B (Japan) electron microscope. RESULTS: Use of avian erythrocytes as a target of adhesion determination, compared with animal erythrocytes, has shown that bacteria can cause D-mannose-sensitive hemagglutination reaction, linked with the presence of 110 - 420 nm long and 5.0 - 5.4 nm wide cilia in the microorganisms. CONCLUSION: Adhesion of enterobacteria was shown to be a complex process, depending on the presence of certain fimbrial structures, use of those results in specific interaction of the microbe with certain host cell receptors. Avian erythrocytes are a model target cells.
Assuntos
Aderência Bacteriana/imunologia , Enterobacteriaceae/patogenicidade , Eritrócitos/microbiologia , Hemaglutinação/imunologia , Animais , Aves/microbiologia , Enterobacteriaceae/imunologia , Eritrócitos/patologia , Eritrócitos/ultraestrutura , Fímbrias Bacterianas/imunologia , Fímbrias Bacterianas/ultraestrutura , Testes de Hemaglutinação , HumanosRESUMO
The objective of the present work was to develop indications for the combined treatment of patients presenting with osteoarthrosis including the use of peloid applications and intramuscular injections of alflutop. The study was based at Yakty-Kul' health resort and included 94 patients at the age from 40 to 59 years. The proposed method was shown to have beneficial effect on the patients' clinical conditions. In addition, the treatment caused a decrease in the level of tumour necrosis factor-alpha (TNF-alpha) that turned out to be related to the articular index.
Assuntos
Anti-Inflamatórios não Esteroides/uso terapêutico , Estâncias para Tratamento de Saúde , Peloterapia/métodos , Osteoartrite/terapia , Fator de Necrose Tumoral alfa/sangue , Adulto , Anti-Inflamatórios não Esteroides/administração & dosagem , Bashkiria , Terapia Combinada , Humanos , Injeções Intramusculares , Articulações/fisiopatologia , Pessoa de Meia-Idade , Osteoartrite/sangue , Osteoartrite/diagnóstico por imagem , Osteoartrite/tratamento farmacológico , Radiografia , Recuperação de Função Fisiológica , Resultado do TratamentoRESUMO
Influence of thermolabile enterotoxin bacteria of Enterobacter genus on the immune system of mice was studied. Assessment of phagocytic functions of the immune system as well as antigen-presenting functions of macrophages during infection with enterotoxin-producing strains of bacteria from Enterobacter genus revealed pleiotropic effect of the toxin which is characterized by inhibition of antigen-presenting and processing functions of macrophages.
