RESUMO
The hydrodynamic behavior observed for a sphere released under gravity in a Newtonian liquid is not consistent with that predicted by classical continuum theory when the sphere is near a solid wall. An irreversibility arises in the velocity of the sphere as it approaches and recedes from the plane that cannot be accounted for using continuum hydrodynamic equations alone. Earlier experiments on spheres falling from a plane were conducted under conditions such that this irreversibility could be attributed to the surface roughness of the spheres. In this investigation, we extend these studies to situations where the pressure field between the receding sphere and the plane drops to the vapor pressure of the fluid and cavitation occurs. Experimental data supports the theoretical prediction for a sphere's motion based on the irreversible effect of cavitation.
RESUMO
We developed the SNPlex Genotyping System to address the need for accurate genotyping data, high sample throughput, study design flexibility, and cost efficiency. The system uses oligonucleotide ligation/polymerase chain reaction and capillary electrophoresis to analyze bi-allelic single nucleotide polymorphism genotypes. It is well suited for single nucleotide polymorphism genotyping efforts in which throughput and cost efficiency are essential. The SNPlex Genotyping System offers a high degree of flexibility and scalability, allowing the selection of custom-defined sets of SNPs for medium- to high-throughput genotyping projects. It is therefore suitable for a broad range of study designs. In this article we describe the principle and applications of the SNPlex Genotyping System, as well as a set of single nucleotide polymorphism selection tools and validated assay resources that accelerate the assay design process. We developed the control pool, an oligonucleotide ligation probe set for training and quality-control purposes, which interrogates 48 SNPs simultaneously. We present performance data from this control pool obtained by testing genomic DNA samples from 44 individuals. in addition, we present data from a study that analyzed 521 SNPs in 92 individuals. Combined, both studies show the SNPlex Genotyping system to have a 99.32% overall call rate, 99.95% precision, and 99.84% concordance with genotypes analyzed by TaqMan probe-based assays. The SNPlex Genotyping System is an efficient and reliable tool for a broad range of genotyping applications, supported by applications for study design, data analysis, and data management.
