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1.
Braz J Med Biol Res ; 37(7): 1095-101, 2004 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15264018

RESUMO

The GLUT4 transporter plays a key role in insulin-induced glucose uptake, which is impaired in insulin resistance. The objective of the present study was to investigate the tissue content and the subcellular distribution of GLUT4 protein in 4- to 12-year-old control, obese and insulin-treated diabetic mongrel female dogs (4 animals per group). The parametrial white adipose tissue was sampled and processed to obtain both plasma membrane and microsome subcellular fractions for GLUT4 analysis by Western blotting. There was no significant difference in glycemia and insulinemia between control and obese animals. Diabetic dogs showed hyperglycemia (369.9 +/- 89.9 mg/dl). Compared to control, the plasma membrane GLUT4, reported per g tissue, was reduced by 55% (P < 0.01) in obese dogs, and increased by 30% (P < 0.05) in diabetic dogs, and the microsomal GLUT4 was increased by approximately 45% (P < 0.001) in both obese and diabetic animals. Considering the sum of GLUT4 measured in plasma membrane and microsome as total cellular GLUT4, percent GLUT4 present in plasma membrane was reduced by approximately 65% (P < 0.001) in obese compared to control and diabetic animals. Since insulin stimulates GLUT4 translocation to the plasma membrane, percent GLUT4 in plasma membrane was divided by the insulinemia at the time of tissue removal and was found to be reduced by 75% (P < 0.01) in obese compared to control dogs. We conclude that the insulin-stimulated translocation of GLUT4 to the cell surface is reduced in obese female dogs. This probably contributes to insulin resistance, which plays an important role in glucose homeostasis in dogs.


Assuntos
Adipócitos/metabolismo , Diabetes Mellitus/veterinária , Doenças do Cão/metabolismo , Proteínas de Transporte de Monossacarídeos/metabolismo , Proteínas Musculares/metabolismo , Obesidade/veterinária , Animais , Transporte Biológico , Western Blotting , Membrana Celular/metabolismo , Diabetes Mellitus/tratamento farmacológico , Diabetes Mellitus/metabolismo , Modelos Animais de Doenças , Doenças do Cão/tratamento farmacológico , Cães , Feminino , Transportador de Glucose Tipo 4 , Hipoglicemiantes/administração & dosagem , Hipoglicemiantes/metabolismo , Insulina/administração & dosagem , Insulina/metabolismo , Microssomos/metabolismo , Proteínas de Transporte de Monossacarídeos/análise , Proteínas Musculares/análise , Obesidade/metabolismo , Ovariectomia/veterinária
2.
Braz. j. med. biol. res ; 37(7): 1095-1101, July 2004. ilus, tab, graf
Artigo em Inglês | LILACS | ID: lil-360936

RESUMO

The GLUT4 transporter plays a key role in insulin-induced glucose uptake, which is impaired in insulin resistance. The objective of the present study was to investigate the tissue content and the subcellular distribution of GLUT4 protein in 4-to 12-year-old control, obese and insulin-treated diabetic mongrel female dogs (4 animals per group). The parametrial white adipose tissue was sampled and processed to obtain both plasma membrane and microsome subcellular fractions for GLUT4 analysis by Western blotting. There was no significant difference in glycemia and insulinemia between control and obese animals. Diabetic dogs showed hyperglycemia (369.9 ± 89.9 mg/dl). Compared to control, the plasma membrane GLUT4, reported per g tissue, was reduced by 55 percent (P < 0.01) in obese dogs, and increased by 30 percent (P < 0.05) in diabetic dogs, and the microsomal GLUT4 was increased by approximately 45 percent (P < 0.001) in both obese and diabetic animals. Considering the sum of GLUT4 measured in plasma membrane and microsome as total cellular GLUT4, percent GLUT4 present in plasma membrane was reduced by approximately 65 percent (P < 0.001) in obese compared to control and diabetic animals. Since insulin stimulates GLUT4 translocation to the plasma membrane, percent GLUT4 in plasma membrane was divided by the insulinemia at the time of tissue removal and was found to be reduced by 75 percent (P < 0.01) in obese compared to control dogs. We conclude that the insulin-stimulated translocation of GLUT4 to the cell surface is reduced in obese female dogs. This probably contributes to insulin resistance, which plays an important role in glucose homeostasis in dogs.


Assuntos
Animais , Feminino , Cães , Adipócitos , Diabetes Mellitus Experimental , Insulina , Obesidade , Transporte Biológico , Western Blotting , Membrana Celular , Modelos Animais de Doenças , Microssomos
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