Development of a three-dimensional computer model of the equine heart using a polyurethane casting technique and in vivo contrast-enhanced computed tomography.

INTRODUCTION/OBJECTIVES: Insight into the three-dimensional (3D) anatomy of the equine heart is essential in veterinary education and to develop minimally invasive intracardiac procedures. The aim was to create a 3D computer model simulating the in vivo anatomy of the adult equine heart. ANIMALS: Ten horses and five ponies. MATERIALS AND METHODS: Ten horses, euthanized for non-cardiovascular reasons, were used for in situ cardiac casting with polyurethane foam and subsequent computed tomography (CT) of the excised heart. In five anaesthetized ponies, a contrast-enhanced electrocardiogram-gated CT protocol was optimized to image the entire heart. Dedicated image processing software was used to create 3D models of all CT scans derived from both methods. Resulting models were compared regarding relative proportions, detail and ease of segmentation. RESULTS: The casting protocol produced high detail, but compliant structures such as the pulmonary trunk were disproportionally expanded by the foam. Optimization of the contrast-enhanced CT protocol, especially adding a delayed phase for visualization of the cardiac veins, resulted in sufficiently detailed CT images to create an anatomically correct 3D model of the pony heart. Rescaling was needed to obtain a horse-sized model. CONCLUSIONS: Three-dimensional computer models based on contrast-enhanced CT images appeared superior to those based on casted hearts to represent the in vivo situation and are preferred to obtain an anatomically correct heart model useful for education, client communication and research purposes. Scaling was, however, necessary to obtain an approximation of an adult horse heart as cardiac CT imaging is restricted by thoracic size.

Effects of Xylo-Oligosaccharides on Broiler Chicken Performance and Microbiota.

In broiler chickens, feed additives, including prebiotics, are widely used to improve gut health and to stimulate performance. Xylo-oligosaccharides (XOS) are hydrolytic degradation products of arabinoxylans that can be fermented by the gut microbiota. In the current study, we aimed to analyze the prebiotic properties of XOS when added to the broiler diet. Administration of XOS to chickens, in addition to a wheat-rye-based diet, significantly improved the feed conversion ratio. XOS significantly increased villus length in the ileum. It also significantly increased numbers of lactobacilli in the colon and Clostridium cluster XIVa in the ceca. Moreover, the number of gene copies encoding the key bacterial enzyme for butyrate production, butyryl-coenzyme A (butyryl-CoA):acetate CoA transferase, was significantly increased in the ceca of chickens administered XOS. In this group of chickens, at the species level, Lactobacillus crispatus and Anaerostipes butyraticus were significantly increased in abundance in the colon and cecum, respectively. In vitro fermentation of XOS revealed cross-feeding between L. crispatus and A. butyraticus. Lactate, produced by L. crispatus during XOS fermentation, was utilized by the butyrate-producing Anaerostipes species. These data show the beneficial effects of XOS on broiler performance when added to the feed, which potentially can be explained by stimulation of butyrate-producing bacteria through cross-feeding of lactate and subsequent effects of butyrate on gastrointestinal function.

[Detection of hyperketonemia with the aid of monitoring milk production and two milk tests]. / Detectie van hyperketonemie met behulp van beoordeling van de melkproductie en twee melktesten.

In addition, it is relevant to know to what extent milk production data can provide information about hyperketonaemia. In 12 selected herds, milk samples were collected from 114 cows that had been lactating for maximally 70 days. During regular milk testing, two additional milk samples were taken to assess the 'Nitroprusside test' and the 'Ketolac BHB' test. After collection of milk samples, blood samples were taken to measure serum beta-hydroxybutyric acid. The prevalence of hyperketonaemia appeared to vary between herds from 1 to 9 per 10 lactating cows. The sensitivity of the Nitroprusside test was higher than the sensitivity of the Ketolac BHB test at threshold values of beta-HBA > 1.5 mmol/l. The Ketolac BHB test in comparison to the Nitroprusside test appeared to provide a smaller chance of false-positive test results at a given level of sensitivity. The serum beta-hydroxybutyric acid concentration was correlated with milk yield/day and the milk fat concentration of an individual cow.

Attending to color with proper fixation.

In contemporary theorizing, there is a controversy about the role of spatial location in the selection of visual information; some theories postulate that position plays a unique role, whereas other theories hold that position is just one selection dimension that is not different from other dimensions, such as color and shape. In this context, a paradigm introduced by Tsal and Lavie (1988) promised to be of fundamental importance. With that paradigm, Tsal and Lavie found that, after reporting a first letter of a prespecified color, subjects preferred to switch their reporting to letters from array positions adjacent to that letter over continuing to report letters of the same color as that of the first letter. This switch from color to position provided firm evidence in favor of the "position-special" views as opposed to the "all-attributes-are-equal" views. In the present study, six experiments, employing Tsal and Lavie's paradigm and variations of that paradigm, are reported. Experiments 1,2,4, and 5 show that evidence for a switch from selection on the basis of color to selection on the basis of position is not obtained when subjects are forced to fixate the fixation point and possibly also not under normal contrast conditions without fixation controls. Experiment 3 shows that switching from color to position is difficult. Experiments 2, 5, and 6 show that evidence for a switch is obtained only under low-contrast conditions when subjects are not forced to fixate the fixation point. It is concluded that the Tsal and Lavie paradigm is an asymmetric paradigm. The results reported by Tsal and Lavie constituted a major threat for the "all-attributes-are-equal" theories and provided firm support for the "position-special" theories. The results reported in the present study are compatible with the all-attributes-are-equal theories, but, at the same time, do not constitute a major threat for the contemporary position-special theories.

Reducing color-color interference by optimizing selection for action.

Color-color interference refers to the finding that the naming of a target color is hampered by the simultaneous presentation of an incongruent distractor color somewhere else in the visual field. This interference effect has been attributed to an imperfect input selection (selection-for-processing). We test an alternative account in which it is assumed that (a) target and distractor are identified in parallel without mutual interference, (b) the identified target color has to be selected to control the naming response (selection-for-action), and (c) this selection process takes more time and is less accurate in the incongruent condition than in the control conditions. Experiment 1 shows that color-color interference obtained when a target color, presented at the point of fixation, is flanked by incongruent colors. In Experiments 2 and 3, the central target position is indicated by an additional exogenous selection cue. The results show that an abrupt-onset cue, presented at the central target position 160 ms after the onset of the target and distractors, reduces the interference effect. This finding is interpreted as supporting evidence for a selection-for-action account of color-color interference.

Cervical intra-epithelial neoplasia and invasive cervical cancer in black and white patients.

The relative incidences of cervical intra-epithelial neoplasia (CIN) and invasive cervical cancer were studied in black and white patients at the academic hospitals of the University of the Orange Free State. A statistically highly significant difference was found between black and white patients, with a higher incidence of invasive cervical cancer than stage III CIN (CIN III) in black patients and a higher incidence of CIN III than invasive cervical cancer in white patients (P = 0.000092; 95% confidence interval -0.355-(-)0.128). The time interval between the peak incidence of CIN III and that of invasive cervical cancer was found to be shorter in black than in white patients. These distressing findings emphasise the urgent need for a national cervical cytological screening programme to decrease the incidence of invasive cervical cancer. This serious yet preventable disease is still very prevalent in South Africa, especially among black women.

Effect of phosphorylation on hydrogen-bonding interactions of the active site histidine of the phosphocarrier protein HPr of the phosphoenolpyruvate-dependent phosphotransferase system determined by 15N NMR spectroscopy.

The phosphocarrier protein HPr of the phosphoenolpyruvate-dependent sugar transport system of Escherichia coli can exist in a phosphorylated and a nonphosphorylated form. During phosphorylation, the phosphoryl group is carried on a histidine residue, His15. The hydrogen-bonding state of this histidine was examined with 15N NMR. For this purpose we selectively enriched the histidine imidazole nitrogens with 15N by supplying an E. coli histidine auxotroph with the amino acid labeled either at the N delta 1 and N epsilon 2 positions or at only the N delta 1 position. 15N NMR spectra of two synthesized model compounds, phosphoimidazole and phosphomethylimidazole, were also recorded. We show that, prior to phosphorylation, the protonated His15 N epsilon 2 is strongly hydrogen bonded, most probably to a carboxylate moiety. The H-bond should strengthen the nucleophilic character of the deprotonated N delta 1, resulting in a good acceptor for the phosphoryl group. The hydrogen bond to the His15 N delta 1 breaks upon phosphorylation of the residue. Implications of the H-bond structure for the mechanism of phosphorylation of HPr are discussed.

Mapping of recombinants near the Huntington disease locus by using G8 (D4S10) and newly isolated markers in the D4S10 region.

Genetic linkage between the marker G8 (D4S10) and Huntington disease (HD) was studied in six Dutch pedigrees. The informativeness of the D4S10 locus was increased by isolation of a cosmid, C5.5, with a G8 subclone used as probe. We present a restriction map of 70 kb in the D4S10 region. Two subclones of C5.5, H5.52 and F5.53, detect MspI and SinI RFLPs, respectively. These probes increase the informativeness of D4S10 in the Dutch HD population from 55% to 95%. Seven recombinations were found in 124 informative meioses in which multipoint segregation of D4S10 haplotypes and the HD locus was studied. Two of the recombinations occurred within the D4S10 region. The other five recombinations are highly valuable for the mapping of present and future markers relative to each other and to the HD gene. In addition, several recombinations between markers in meioses from unaffected parents were noted, which will also be useful in ordering new markers. On the basis of our three-point recombination data, the orientation of the D4S10 region relative to HD is HD-H5.52-G8-F5.53, which independently confirms the previously derived polarity for D4S10.

DNA probe analysis for carrier detection and prenatal diagnosis of Duchenne muscular dystrophy: a standard diagnostic procedure.

Thirteen marker loci localised on the short arm of the X chromosome are available for use in genetic studies for Duchenne muscular dystrophy (DMD). This large number of probes detecting about 20 RFLPs encouraged us to set up a standard procedure using a sequence of selected probes and restriction enzymes for the diagnosis of DMD families. The application of DNA probe analysis for carrier detection and prenatal diagnosis, involving 61 pedigrees of both familial and isolated cases, has yielded the following results. Carrier detection using flanking markers was possible in more than 75% of the cases (104 out of 136 females) with a reliability of better than 98%. Prenatal diagnosis was possible in 95% of the cases (65 out of 68 proven carriers or women at risk). Twenty-three prenatal diagnoses were performed on male fetuses; 13 appeared to have a low risk for DMD (less than 1%) and thus the pregnancies continued. Seven have since come to term and the male infants have normal CK levels. The genetic distances of the loci relative to the DMD locus and their order on the short arm of the X chromosome were deduced from our total DMD family material and are not significantly different from those reported earlier. For 754 (DXS84) we found a genetic distance of 5 cM with a lod score of +12.4 and 95% confidence limits between 2 and 12 cM. Similar data were obtained for pERT87 (DXS164), suggesting that in our family material both loci are tightly linked. Multiply informative recombination showed that both 754 and pERT87 map proximal to the DMD mutations in the cases studied. The high frequency of DMD mutations and its relation to the observed instability in this part of the genome will be discussed. Unequal crossing over is proposed as one of the mechanisms contributing to the high mutation frequency.