A unique case of large cell calcifying type sertoli tumor.

Sertoli cells tumors are a rare testicular malignancy, with prevalence estimated to be less than 1% of testicular cancers. Furthermore, testicular cancer only accounts for approximately 0.5% of all diagnosed cancers. This is a unique case of a 19-year-old male where physical exam and ultrasonography findings revealed an intratesticular mass that, after pathologic analysis, demonstrated T1 large cell calcifying type Sertoli cell tumor which is infrequently documented in the literature.

Metastatic melanoma to the proximal ureter.

We present an exceptionally rare scenario of obstructive uropathy secondary to a ureteral mass. This case details a 68-year-old female with metastatic stage IIID melanoma of the right heel found to have a two cm right proximal ureteral mass with associated hydronephrosis. Pathology from ureteroscopic biopsy revealed metastatic melanoma to the ureter, creating a unique treatment quandary.

A rare case of follicular lymphoma of the bladder.

This case details a 69-year-old female with a 3cm bladder mass on imaging who underwent transurethral resection of the bladder with pathology revealing non-Hodgkin follicular lymphoma to the bladder. The rarity and complexity of this presentation generated a challenging treatment dilemma regarding surveillance and active treatment.

An unusual case of spontaneous rupture of the renal pelvis.

Spontaneous rupture of the renal pelvis is a relatively rare urologic finding. It is associated with obstructing ureteral calculi and can occur with or without urinary instrumentation. Spontaneous rupture is typically diagnosed through imaging modalities such as computed tomography (CT) scans or retrograde pyelography. In this case report, we detail a case regarding spontaneous rupture of the renal pelvis (SRRP) of a 33-year-old male with idiopathic extravasation of contrast from the renal pelvis discovered on delayed phase CT scan of the abdomen and pelvis. This is a unique case of SRRP as the etiology remains idiopathic.

The role of miR-150 regulates bone cell differentiation and function.

BACKGROUND: mir-RNAs play a role in regulating bone homeostasis. In this study we assessed the functional role of mir-RNA 150 in bone homeostasis. We also assess the effects of miR-150 deficiency on osteoblast and osteoclast differentiation and function using in vivo and in vitro approaches. METHODS: Wild type (WT) (C57BL/6J) and miR-150 KO mice were compared for a variety of parameters. Micro-CT imaging was conducted to quantify trabecular bone mass inferior to the distal growth plate of the femur. Von Kossa staining was performed for osteoblast culture mineralization. RT-qPCR, biochemical analysis and bone histomorphometry were utilized for quantification of relevant genes and serum protein measurements. Differentiation and function of osteoblasts and osteoclasts was performed using primarily cultures and assessed the cell autonomous response of mir-RNA-150 on cell differentiation and function. RESULTS: Mir-150 exhibited expression in a variety of tissues and increases progressively with age. Through micro-CT imaging, we found that KO mice presented reduced bone mass at 4, 8, and 16 weeks of age compared to WT mice. Furthermore, histomorphometric analysis revealed increased trabecular separation, decreased bone thickness, and decreased osteoblast number in KO compared to WT mice. Mir-150 deficiency also correlated with higher bone resorption, accompanied with significant increases in CTX-1 serum levels, and a decrease in cell apoptotic rate ex vivo. Additionally, miR-150 KO mice showed increased osteoblast differentiation and decreased osteoclastogenesis ex vivo. Luciferase assay showed increased Osteoactivin/GPNMB expression in miR-150 KO osteoblasts compared to WT cells. CONCLUSION: Our data suggests that miR-150 influences osteoblast and osteoclast functionality and differentiation; specifically, miR-150 serves as a negative regulator for osteoblasts and a positive regulator for osteoclasts by regulating, at least in part, Osteoactivin/GPNMB expression.