RESUMO
A significant decrease in the baseline of sister-chromatid exchanges (SCEs) was observed in cultured human lymphocytes, if 5-bromodeoxyuridine (BrdU) was added after 60 h of culture, and the cells were harvested at least 24-30 h after BrdU exposure. This decrease is supposed to occur if at least one cell division takes place before the addition of BrdU. For cytogenetic monitoring of mutagenic environmental factors, using human lymphocyte cultures, it is assumed that two time periods are sufficient for comparison.
Assuntos
Sobrevivência Celular , Troca Genética , Linfócitos/citologia , Troca de Cromátide Irmã , Bromodesoxiuridina , Células Cultivadas , Feminino , Humanos , MasculinoRESUMO
The spontaneous level of sister chromatide exchanges (SCE) registered in human lymphocytes is shown to depend on the moment of BUdR introduction and the time of fixation. In early periods of BUdR introduction and fixation the general spontaneous level of SCE may be observed and in later periods only that part of SCE may be registered which is caused by internal conditions. The difference between the first and second results makes the part of SCE conditioned by the environmental effects.
Assuntos
Troca Genética , Troca de Cromátide Irmã , Adulto , Bromodesoxiuridina/farmacologia , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Troca Genética/efeitos dos fármacos , Humanos , Recém-Nascido , Ativação Linfocitária/efeitos dos fármacos , Linfócitos/efeitos dos fármacos , Linfócitos/ultraestrutura , Fito-Hemaglutininas/farmacologia , Troca de Cromátide Irmã/efeitos dos fármacos , Fatores de TempoRESUMO
The sister chromatid exchange (SCE) level in patients with cancer of lungs before treatment did not differ from the mean number of SCE in healthy donors. In the process of a cyclophosphane treatment the SCE frequency in the patient lymphocyte culture increased with the drug doses of 4 and 5 g. Analysis of the ratio of cells in mitoses I, II and III revealed no differences in the cell cycle rate for groups of healthy donors, patients before the treatment, and treated patients.
