RESUMO
OBJECTIVES: The purpose of the study was to evaluate the effect of different types of fluoride on tensile properties and surface roughness of titanium based archwires clinically. MATERIALS AND METHODS: Three groups of archwires, namely nickel-titanium (NiTi), heat activated NiTi, and TMA was evaluated clinically. Each group comprised four subgroups, namely as received group, nonfluoride control group, APF gel group (received one application of 1.23% APF gel), and sodium fluoride mouthwash group (patients rinsed twice daily with 0.2% sodium fluoride mouthwash). All the archwires were removed after 3 weeks of clinical use and evaluated for surface roughness with three dimensional optical profiler. Tensile strength and elongation rate was determined with Instron universal testing machine. RESULTS: NiTi, heat activated NiTi, and TMA archwires with APF gel application showed the highest reduction in tensile strength and elongation rate and highest value for surface roughness, followed by sodium fluoride mouthwash group and finally wires without fluoride application. NiTi wires had the highest reduction in tensile strength with APF gel followed by heat activated NiTi and TMA. CONCLUSION: APF gel with highest concentration of fluoride caused most degradation in the tensile properties and surface roughness of titanium based arch wires.
RESUMO
Foot-and-mouth disease (FMD) is a contagious viral disease affecting cloven hoofed livestock. Insect cell expressed virus like particles (VLPs) are potential alternative to overcome the limitations of inactivated vaccine. However, at pH < 6.5, virus particles disassociate into pentameric structure resulting in loss of antigenicity. Accordingly, we generated seven mutant VLPs containing mutations in the structural genes of FMDV vaccine strains (N17D and/or H145Y for serotypes O/IND/R2/75 and Asia1/IND/63/72; and H142D for serotype A/IND/40/00) by PCR based site directed mutagenesis. Acid resistant VLPs produced by baculovirus expression system were tested for acid stability at pH 7.5, 6.5, 6.0 and 5.5 followed by reactivity in sandwich-ELISA (s-ELISA), which revealed mutant-1 (N17D) of serotype O and Asia1 retained the antigenicity in s-ELISA even at pH 5.5 as compared to other VLPs and wild-types. Further, the 75S empty capsids obtained in sucrose density gradient, when tested in liquid phase blocking ELISA (LPBE) in comparison to cell culture antigen indicated that the VLPs were stable at acidic pH. Transmission electron microscopy of OM-1 confirmed the intact morphology of the empty VLPs. It is concluded that acid resistant VLPs could be useful for developing new generation vaccine or diagnostic for FMDV.